Tailored cell sheet engineering using microstereolithography and electrochemical cell transfer

Abstract Postoperative adhesion and occlusion remain a serious issue associated with various surgeries, including endoscopic surgery, in which proliferated fibrous tissues stick to adjacent tissues and often cause severe complications. Cell sheet engineering has emerged as an effective approach not...

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Autores principales:	Yuka Kobayashi, Christopher E. J. Cordonier, Yohei Noda, Fuminori Nagase, Junko Enomoto, Tatsuto Kageyama, Hideo Honma, Shoji Maruo, Junji Fukuda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9433bcf349684c7f89047484a1885619
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spelling	oai:doaj.org-article:9433bcf349684c7f89047484a18856192021-12-02T15:09:53ZTailored cell sheet engineering using microstereolithography and electrochemical cell transfer10.1038/s41598-019-46801-92045-2322https://doaj.org/article/9433bcf349684c7f89047484a18856192019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46801-9https://doaj.org/toc/2045-2322Abstract Postoperative adhesion and occlusion remain a serious issue associated with various surgeries, including endoscopic surgery, in which proliferated fibrous tissues stick to adjacent tissues and often cause severe complications. Cell sheet engineering has emerged as an effective approach not only for cell transplantation but also for the treatment of postoperative adhesion and occlusion. However, as the tissues in the body, such as middle ear and small intestine, and typical operative sites are non-flat and spatially complicated, tailored cell sheets with three-dimensional (3D) configurations may lead to widespread use of this approach. In the present study, we used microstereolithography, biocompatible gold plating, and electrochemical cell detachment to achieve this purpose. Various objects with dimensions ranging from millimeter- to micrometer-scale were fabricated with photocurable resin using lab-made equipment for microstereolithography. To coat the fabricated objects with a thin gold layer, conventional cyanide-based gold plating was unusable because it severely damaged almost all cells. Electroless non-cyanide gold plating we prepared was cytocompatible and suitable for electrochemical cell detachment. Cell sheets on the gold-plated substrate could be directly transplanted into a mouse intraperitoneally using electrochemical cell detachment. We further demonstrated that cell sheets grown on gold-coated 3D objects were rapidly detached along with the desorption of electroactive-oligopeptide monolayer and transferred to a surrounding hydrogel. This approach may provide a promising strategy to prepare and directly transplant tailor-made cell sheets with suitable configurations.Yuka KobayashiChristopher E. J. CordonierYohei NodaFuminori NagaseJunko EnomotoTatsuto KageyamaHideo HonmaShoji MaruoJunji FukudaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuka Kobayashi Christopher E. J. Cordonier Yohei Noda Fuminori Nagase Junko Enomoto Tatsuto Kageyama Hideo Honma Shoji Maruo Junji Fukuda Tailored cell sheet engineering using microstereolithography and electrochemical cell transfer
description	Abstract Postoperative adhesion and occlusion remain a serious issue associated with various surgeries, including endoscopic surgery, in which proliferated fibrous tissues stick to adjacent tissues and often cause severe complications. Cell sheet engineering has emerged as an effective approach not only for cell transplantation but also for the treatment of postoperative adhesion and occlusion. However, as the tissues in the body, such as middle ear and small intestine, and typical operative sites are non-flat and spatially complicated, tailored cell sheets with three-dimensional (3D) configurations may lead to widespread use of this approach. In the present study, we used microstereolithography, biocompatible gold plating, and electrochemical cell detachment to achieve this purpose. Various objects with dimensions ranging from millimeter- to micrometer-scale were fabricated with photocurable resin using lab-made equipment for microstereolithography. To coat the fabricated objects with a thin gold layer, conventional cyanide-based gold plating was unusable because it severely damaged almost all cells. Electroless non-cyanide gold plating we prepared was cytocompatible and suitable for electrochemical cell detachment. Cell sheets on the gold-plated substrate could be directly transplanted into a mouse intraperitoneally using electrochemical cell detachment. We further demonstrated that cell sheets grown on gold-coated 3D objects were rapidly detached along with the desorption of electroactive-oligopeptide monolayer and transferred to a surrounding hydrogel. This approach may provide a promising strategy to prepare and directly transplant tailor-made cell sheets with suitable configurations.
format	article
author	Yuka Kobayashi Christopher E. J. Cordonier Yohei Noda Fuminori Nagase Junko Enomoto Tatsuto Kageyama Hideo Honma Shoji Maruo Junji Fukuda
author_facet	Yuka Kobayashi Christopher E. J. Cordonier Yohei Noda Fuminori Nagase Junko Enomoto Tatsuto Kageyama Hideo Honma Shoji Maruo Junji Fukuda
author_sort	Yuka Kobayashi
title	Tailored cell sheet engineering using microstereolithography and electrochemical cell transfer
title_short	Tailored cell sheet engineering using microstereolithography and electrochemical cell transfer
title_full	Tailored cell sheet engineering using microstereolithography and electrochemical cell transfer
title_fullStr	Tailored cell sheet engineering using microstereolithography and electrochemical cell transfer
title_full_unstemmed	Tailored cell sheet engineering using microstereolithography and electrochemical cell transfer
title_sort	tailored cell sheet engineering using microstereolithography and electrochemical cell transfer
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/9433bcf349684c7f89047484a1885619
work_keys_str_mv	AT yukakobayashi tailoredcellsheetengineeringusingmicrostereolithographyandelectrochemicalcelltransfer AT christopherejcordonier tailoredcellsheetengineeringusingmicrostereolithographyandelectrochemicalcelltransfer AT yoheinoda tailoredcellsheetengineeringusingmicrostereolithographyandelectrochemicalcelltransfer AT fuminorinagase tailoredcellsheetengineeringusingmicrostereolithographyandelectrochemicalcelltransfer AT junkoenomoto tailoredcellsheetengineeringusingmicrostereolithographyandelectrochemicalcelltransfer AT tatsutokageyama tailoredcellsheetengineeringusingmicrostereolithographyandelectrochemicalcelltransfer AT hideohonma tailoredcellsheetengineeringusingmicrostereolithographyandelectrochemicalcelltransfer AT shojimaruo tailoredcellsheetengineeringusingmicrostereolithographyandelectrochemicalcelltransfer AT junjifukuda tailoredcellsheetengineeringusingmicrostereolithographyandelectrochemicalcelltransfer
_version_	1718387766457270272

Tailored cell sheet engineering using microstereolithography and electrochemical cell transfer

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