High-fat diet induces periodontitis in mice through lipopolysaccharides (LPS) receptor signaling: protective action of estrogens.

<h4>Background</h4>A fat-enriched diet favors the development of gram negative bacteria in the intestine which is linked to the occurrence of type 2 diabetes (T2D). Interestingly, some pathogenic gram negative bacteria are commonly associated with the development of periodontitis which,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vincent Blasco-Baque, Matteo Serino, Jean-Noël Vergnes, Elodie Riant, Pascale Loubieres, Jean-François Arnal, Pierre Gourdy, Michel Sixou, Rémy Burcelin, Philippe Kemoun
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/943504b438c6422d98310751a9a4c803
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:943504b438c6422d98310751a9a4c803
record_format	dspace
spelling	oai:doaj.org-article:943504b438c6422d98310751a9a4c8032021-11-18T08:10:10ZHigh-fat diet induces periodontitis in mice through lipopolysaccharides (LPS) receptor signaling: protective action of estrogens.1932-620310.1371/journal.pone.0048220https://doaj.org/article/943504b438c6422d98310751a9a4c8032012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23133617/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>A fat-enriched diet favors the development of gram negative bacteria in the intestine which is linked to the occurrence of type 2 diabetes (T2D). Interestingly, some pathogenic gram negative bacteria are commonly associated with the development of periodontitis which, like T2D, is characterized by a chronic low-grade inflammation. Moreover, estrogens have been shown to regulate glucose homeostasis via an LPS receptor dependent immune-modulation. In this study, we evaluated whether diet-induced metabolic disease would favor the development of periodontitis in mice. In addition, the regulatory role of estrogens in this process was assessed.<h4>Methods</h4>Four-week-old C57BL6/J WT and CD14 (part of the TLR-4 machinery for LPS-recognition) knock-out female mice were ovariectomised and subcutaneously implanted with pellets releasing either placebo or 17β-estradiol (E2). Mice were then fed with either a normal chow or a high-fat diet for four weeks. The development of diabetes was monitored by an intraperitoneal glucose-tolerance test and plasma insulin concentration while periodontitis was assessed by identification of pathogens, quantification of periodontal soft tissue inflammation and alveolar bone loss.<h4>Results</h4>The fat-enriched diet increased the prevalence of periodontal pathogenic microbiota like Fusobacterium nucleatum and Prevotella intermedia, gingival inflammation and alveolar bone loss. E2 treatment prevented this effect and CD14 knock-out mice resisted high-fat diet-induced periodontal defects.<h4>Conclusions/significance</h4>Our data show that mice fed with a diabetogenic diet developed defects and microflora of tooth supporting-tissues typically associated with periodontitis. Moreover, our results suggest a causal link between the activation of the LPS pathway on innate immunity by periodontal microbiota and HFD-induced periodontitis, a pathophysiological mechanism that could be targeted by estrogens.Vincent Blasco-BaqueMatteo SerinoJean-Noël VergnesElodie RiantPascale LoubieresJean-François ArnalPierre GourdyMichel SixouRémy BurcelinPhilippe KemounPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48220 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vincent Blasco-Baque Matteo Serino Jean-Noël Vergnes Elodie Riant Pascale Loubieres Jean-François Arnal Pierre Gourdy Michel Sixou Rémy Burcelin Philippe Kemoun High-fat diet induces periodontitis in mice through lipopolysaccharides (LPS) receptor signaling: protective action of estrogens.
description	<h4>Background</h4>A fat-enriched diet favors the development of gram negative bacteria in the intestine which is linked to the occurrence of type 2 diabetes (T2D). Interestingly, some pathogenic gram negative bacteria are commonly associated with the development of periodontitis which, like T2D, is characterized by a chronic low-grade inflammation. Moreover, estrogens have been shown to regulate glucose homeostasis via an LPS receptor dependent immune-modulation. In this study, we evaluated whether diet-induced metabolic disease would favor the development of periodontitis in mice. In addition, the regulatory role of estrogens in this process was assessed.<h4>Methods</h4>Four-week-old C57BL6/J WT and CD14 (part of the TLR-4 machinery for LPS-recognition) knock-out female mice were ovariectomised and subcutaneously implanted with pellets releasing either placebo or 17β-estradiol (E2). Mice were then fed with either a normal chow or a high-fat diet for four weeks. The development of diabetes was monitored by an intraperitoneal glucose-tolerance test and plasma insulin concentration while periodontitis was assessed by identification of pathogens, quantification of periodontal soft tissue inflammation and alveolar bone loss.<h4>Results</h4>The fat-enriched diet increased the prevalence of periodontal pathogenic microbiota like Fusobacterium nucleatum and Prevotella intermedia, gingival inflammation and alveolar bone loss. E2 treatment prevented this effect and CD14 knock-out mice resisted high-fat diet-induced periodontal defects.<h4>Conclusions/significance</h4>Our data show that mice fed with a diabetogenic diet developed defects and microflora of tooth supporting-tissues typically associated with periodontitis. Moreover, our results suggest a causal link between the activation of the LPS pathway on innate immunity by periodontal microbiota and HFD-induced periodontitis, a pathophysiological mechanism that could be targeted by estrogens.
format	article
author	Vincent Blasco-Baque Matteo Serino Jean-Noël Vergnes Elodie Riant Pascale Loubieres Jean-François Arnal Pierre Gourdy Michel Sixou Rémy Burcelin Philippe Kemoun
author_facet	Vincent Blasco-Baque Matteo Serino Jean-Noël Vergnes Elodie Riant Pascale Loubieres Jean-François Arnal Pierre Gourdy Michel Sixou Rémy Burcelin Philippe Kemoun
author_sort	Vincent Blasco-Baque
title	High-fat diet induces periodontitis in mice through lipopolysaccharides (LPS) receptor signaling: protective action of estrogens.
title_short	High-fat diet induces periodontitis in mice through lipopolysaccharides (LPS) receptor signaling: protective action of estrogens.
title_full	High-fat diet induces periodontitis in mice through lipopolysaccharides (LPS) receptor signaling: protective action of estrogens.
title_fullStr	High-fat diet induces periodontitis in mice through lipopolysaccharides (LPS) receptor signaling: protective action of estrogens.
title_full_unstemmed	High-fat diet induces periodontitis in mice through lipopolysaccharides (LPS) receptor signaling: protective action of estrogens.
title_sort	high-fat diet induces periodontitis in mice through lipopolysaccharides (lps) receptor signaling: protective action of estrogens.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/943504b438c6422d98310751a9a4c803
work_keys_str_mv	AT vincentblascobaque highfatdietinducesperiodontitisinmicethroughlipopolysaccharideslpsreceptorsignalingprotectiveactionofestrogens AT matteoserino highfatdietinducesperiodontitisinmicethroughlipopolysaccharideslpsreceptorsignalingprotectiveactionofestrogens AT jeannoelvergnes highfatdietinducesperiodontitisinmicethroughlipopolysaccharideslpsreceptorsignalingprotectiveactionofestrogens AT elodieriant highfatdietinducesperiodontitisinmicethroughlipopolysaccharideslpsreceptorsignalingprotectiveactionofestrogens AT pascaleloubieres highfatdietinducesperiodontitisinmicethroughlipopolysaccharideslpsreceptorsignalingprotectiveactionofestrogens AT jeanfrancoisarnal highfatdietinducesperiodontitisinmicethroughlipopolysaccharideslpsreceptorsignalingprotectiveactionofestrogens AT pierregourdy highfatdietinducesperiodontitisinmicethroughlipopolysaccharideslpsreceptorsignalingprotectiveactionofestrogens AT michelsixou highfatdietinducesperiodontitisinmicethroughlipopolysaccharideslpsreceptorsignalingprotectiveactionofestrogens AT remyburcelin highfatdietinducesperiodontitisinmicethroughlipopolysaccharideslpsreceptorsignalingprotectiveactionofestrogens AT philippekemoun highfatdietinducesperiodontitisinmicethroughlipopolysaccharideslpsreceptorsignalingprotectiveactionofestrogens
_version_	1718422122552885248

High-fat diet induces periodontitis in mice through lipopolysaccharides (LPS) receptor signaling: protective action of estrogens.

Ejemplares similares