Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds

Abstract The endemic strain of Leishmania donovani in Sri Lanka causes cutaneous leishmaniasis (CL) rather than more common visceral form. We have visualized biofilms and profiled the microbiome of lesions and unaffected skin in thirty-nine CL patients. Twenty-four lesions (61.5%) were biofilm-posit...

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Autores principales:	T. D. Jayasena Kaluarachchi, Paul M. Campbell, Rajitha Wickremasinghe, Shalindra Ranasinghe, Renu Wickremasinghe, Surangi Yasawardene, Hiromel De Silva, Chandrani Menike, M. C. K. Jayarathne, Subodha Jayathilake, Ayomi Dilhari, Andrew J. McBain, Manjula M. Weerasekera
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/943638463967409aa9b8f30715a6ea94
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spelling	oai:doaj.org-article:943638463967409aa9b8f30715a6ea942021-12-05T12:15:26ZDistinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds10.1038/s41598-021-02388-82045-2322https://doaj.org/article/943638463967409aa9b8f30715a6ea942021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02388-8https://doaj.org/toc/2045-2322Abstract The endemic strain of Leishmania donovani in Sri Lanka causes cutaneous leishmaniasis (CL) rather than more common visceral form. We have visualized biofilms and profiled the microbiome of lesions and unaffected skin in thirty-nine CL patients. Twenty-four lesions (61.5%) were biofilm-positive according to fluorescence in situ hybridization. Biopsies of biofilm-positive lesions were dominated by Pseudomonas, class Bacilli and Enterobacteriaceae and distinguished by significantly lower community evenness. Higher relative abundance of a class Bacilli OTU was detected in wound swabs versus contralateral skin. Wound swabs and biopsies had significantly distinct microbiome profiles and lower diversity compared to unaffected skin. Greater abundances of potentially pathogenic organisms were observed in wet ulcers, lesions with high parasite loads and large wounds. In summary, more than half of L. donovani associated CL wounds harboured biofilms and the wounds exhibited a distinct, less diverse, microbiome than unaffected skin.T. D. Jayasena KaluarachchiPaul M. CampbellRajitha WickremasingheShalindra RanasingheRenu WickremasingheSurangi YasawardeneHiromel De SilvaChandrani MenikeM. C. K. JayarathneSubodha JayathilakeAyomi DilhariAndrew J. McBainManjula M. WeerasekeraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q T. D. Jayasena Kaluarachchi Paul M. Campbell Rajitha Wickremasinghe Shalindra Ranasinghe Renu Wickremasinghe Surangi Yasawardene Hiromel De Silva Chandrani Menike M. C. K. Jayarathne Subodha Jayathilake Ayomi Dilhari Andrew J. McBain Manjula M. Weerasekera Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds
description	Abstract The endemic strain of Leishmania donovani in Sri Lanka causes cutaneous leishmaniasis (CL) rather than more common visceral form. We have visualized biofilms and profiled the microbiome of lesions and unaffected skin in thirty-nine CL patients. Twenty-four lesions (61.5%) were biofilm-positive according to fluorescence in situ hybridization. Biopsies of biofilm-positive lesions were dominated by Pseudomonas, class Bacilli and Enterobacteriaceae and distinguished by significantly lower community evenness. Higher relative abundance of a class Bacilli OTU was detected in wound swabs versus contralateral skin. Wound swabs and biopsies had significantly distinct microbiome profiles and lower diversity compared to unaffected skin. Greater abundances of potentially pathogenic organisms were observed in wet ulcers, lesions with high parasite loads and large wounds. In summary, more than half of L. donovani associated CL wounds harboured biofilms and the wounds exhibited a distinct, less diverse, microbiome than unaffected skin.
format	article
author	T. D. Jayasena Kaluarachchi Paul M. Campbell Rajitha Wickremasinghe Shalindra Ranasinghe Renu Wickremasinghe Surangi Yasawardene Hiromel De Silva Chandrani Menike M. C. K. Jayarathne Subodha Jayathilake Ayomi Dilhari Andrew J. McBain Manjula M. Weerasekera
author_facet	T. D. Jayasena Kaluarachchi Paul M. Campbell Rajitha Wickremasinghe Shalindra Ranasinghe Renu Wickremasinghe Surangi Yasawardene Hiromel De Silva Chandrani Menike M. C. K. Jayarathne Subodha Jayathilake Ayomi Dilhari Andrew J. McBain Manjula M. Weerasekera
author_sort	T. D. Jayasena Kaluarachchi
title	Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds
title_short	Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds
title_full	Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds
title_fullStr	Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds
title_full_unstemmed	Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds
title_sort	distinct microbiome profiles and biofilms in leishmania donovani-driven cutaneous leishmaniasis wounds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/943638463967409aa9b8f30715a6ea94
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Distinct microbiome profiles and biofilms in Leishmania donovani-driven cutaneous leishmaniasis wounds

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