Molecular and Epidemiological Characteristics of Carbapenemase-Producing <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clinical Isolates in Japan

ABSTRACT Carbapenemase-producing Enterobacteriaceae represent a serious public health threat worldwide. Carbapenemase genes, harbored on a transferable plasmid, have been isolated globally with distinct geographical features. Klebsiella pneumoniae, included in Enterobacteriaceae, also produces carba...

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Autores principales:	Shinsuke Yonekawa, Tomoki Mizuno, Ryuichi Nakano, Akiyo Nakano, Yuki Suzuki, Tomoko Asada, Ayako Ishii, Naoki Kakuta, Kosuke Tsubaki, Sayaka Mizuno, Miho Ogawa, Hisakazu Yano, Kei Kasahara, Keiichi Mikasa
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Klebsiella pneumoniae carbapenemase hypervirulent clones Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9438b39855794246805291b48184edd9
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spelling	oai:doaj.org-article:9438b39855794246805291b48184edd92021-11-15T15:30:58ZMolecular and Epidemiological Characteristics of Carbapenemase-Producing <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clinical Isolates in Japan10.1128/mSphere.00490-202379-5042https://doaj.org/article/9438b39855794246805291b48184edd92020-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00490-20https://doaj.org/toc/2379-5042ABSTRACT Carbapenemase-producing Enterobacteriaceae represent a serious public health threat worldwide. Carbapenemase genes, harbored on a transferable plasmid, have been isolated globally with distinct geographical features. Klebsiella pneumoniae, included in Enterobacteriaceae, also produces carbapenemase and often shows hypervirulence. Overlapping carbapenem resistance and hypervirulence in K. pneumoniae have been reported, but such strains have not yet been found in Japan. Here, we screened 104 carbapenemase-producing K. pneumoniae isolates collected from 37 hospitals and outpatient clinics in Japan between September 2014 and July 2015. PCR and DNA sequencing demonstrated IMP-1 in 21 isolates and IMP-6 in 83 isolates, 77 of which coharbored CTX-M-2. Most of the isolates showed low MICs toward imipenem and meropenem but high MICs toward penicillin and cephalosporins. Conjugation experiments with an Escherichia coli J53 recipient showed that most of the plasmids in IMP-6 producers were transferable, whereas only one-half of the plasmids in IMP-1 producers were transferable. PCR-based replicon typing and multiplex PCR identified five isolates belonging to the CG258 non-tonB79 cluster and no isolate belonging to the CG258-tonB79 cluster or sequence type 307 (ST307). Four K1-ST23 isolates, 10 K2-ST65 isolates, and 7 K2-ST86 isolates were detected that harbored virulence genes. The resistance genes in 85 isolates were transferable, but the virulence genes were not transferred. These results demonstrate the acquisition of IMP-type carbapenemase genes and CTX-M-type genes among hypervirulence isolates in Japan, warranting further attention and countermeasures. In this study, we have determined the molecular characteristics and epidemiology of IMP-6 producers that coharbored various CTX-M genes in Japan. IMPORTANCE Carbapenems serve as a last resort for the clinical treatment of multidrug-resistant infections. Therefore, the rapid spread of carbapenemase-producing strains represents a serious public health threat, further limiting antibiotic choices. The current findings of hypervirulent carbapenemase-producing Klebsiella pneumoniae clinical isolates in Japan demonstrate the potential broad spread and transfer of these genes, necessitating close surveillance.Shinsuke YonekawaTomoki MizunoRyuichi NakanoAkiyo NakanoYuki SuzukiTomoko AsadaAyako IshiiNaoki KakutaKosuke TsubakiSayaka MizunoMiho OgawaHisakazu YanoKei KasaharaKeiichi MikasaAmerican Society for MicrobiologyarticleKlebsiella pneumoniaecarbapenemasehypervirulent clonesMicrobiologyQR1-502ENmSphere, Vol 5, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Klebsiella pneumoniae carbapenemase hypervirulent clones Microbiology QR1-502
spellingShingle	Klebsiella pneumoniae carbapenemase hypervirulent clones Microbiology QR1-502 Shinsuke Yonekawa Tomoki Mizuno Ryuichi Nakano Akiyo Nakano Yuki Suzuki Tomoko Asada Ayako Ishii Naoki Kakuta Kosuke Tsubaki Sayaka Mizuno Miho Ogawa Hisakazu Yano Kei Kasahara Keiichi Mikasa Molecular and Epidemiological Characteristics of Carbapenemase-Producing <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clinical Isolates in Japan
description	ABSTRACT Carbapenemase-producing Enterobacteriaceae represent a serious public health threat worldwide. Carbapenemase genes, harbored on a transferable plasmid, have been isolated globally with distinct geographical features. Klebsiella pneumoniae, included in Enterobacteriaceae, also produces carbapenemase and often shows hypervirulence. Overlapping carbapenem resistance and hypervirulence in K. pneumoniae have been reported, but such strains have not yet been found in Japan. Here, we screened 104 carbapenemase-producing K. pneumoniae isolates collected from 37 hospitals and outpatient clinics in Japan between September 2014 and July 2015. PCR and DNA sequencing demonstrated IMP-1 in 21 isolates and IMP-6 in 83 isolates, 77 of which coharbored CTX-M-2. Most of the isolates showed low MICs toward imipenem and meropenem but high MICs toward penicillin and cephalosporins. Conjugation experiments with an Escherichia coli J53 recipient showed that most of the plasmids in IMP-6 producers were transferable, whereas only one-half of the plasmids in IMP-1 producers were transferable. PCR-based replicon typing and multiplex PCR identified five isolates belonging to the CG258 non-tonB79 cluster and no isolate belonging to the CG258-tonB79 cluster or sequence type 307 (ST307). Four K1-ST23 isolates, 10 K2-ST65 isolates, and 7 K2-ST86 isolates were detected that harbored virulence genes. The resistance genes in 85 isolates were transferable, but the virulence genes were not transferred. These results demonstrate the acquisition of IMP-type carbapenemase genes and CTX-M-type genes among hypervirulence isolates in Japan, warranting further attention and countermeasures. In this study, we have determined the molecular characteristics and epidemiology of IMP-6 producers that coharbored various CTX-M genes in Japan. IMPORTANCE Carbapenems serve as a last resort for the clinical treatment of multidrug-resistant infections. Therefore, the rapid spread of carbapenemase-producing strains represents a serious public health threat, further limiting antibiotic choices. The current findings of hypervirulent carbapenemase-producing Klebsiella pneumoniae clinical isolates in Japan demonstrate the potential broad spread and transfer of these genes, necessitating close surveillance.
format	article
author	Shinsuke Yonekawa Tomoki Mizuno Ryuichi Nakano Akiyo Nakano Yuki Suzuki Tomoko Asada Ayako Ishii Naoki Kakuta Kosuke Tsubaki Sayaka Mizuno Miho Ogawa Hisakazu Yano Kei Kasahara Keiichi Mikasa
author_facet	Shinsuke Yonekawa Tomoki Mizuno Ryuichi Nakano Akiyo Nakano Yuki Suzuki Tomoko Asada Ayako Ishii Naoki Kakuta Kosuke Tsubaki Sayaka Mizuno Miho Ogawa Hisakazu Yano Kei Kasahara Keiichi Mikasa
author_sort	Shinsuke Yonekawa
title	Molecular and Epidemiological Characteristics of Carbapenemase-Producing <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clinical Isolates in Japan
title_short	Molecular and Epidemiological Characteristics of Carbapenemase-Producing <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clinical Isolates in Japan
title_full	Molecular and Epidemiological Characteristics of Carbapenemase-Producing <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clinical Isolates in Japan
title_fullStr	Molecular and Epidemiological Characteristics of Carbapenemase-Producing <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clinical Isolates in Japan
title_full_unstemmed	Molecular and Epidemiological Characteristics of Carbapenemase-Producing <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clinical Isolates in Japan
title_sort	molecular and epidemiological characteristics of carbapenemase-producing <named-content content-type="genus-species">klebsiella pneumoniae</named-content> clinical isolates in japan
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/9438b39855794246805291b48184edd9
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Molecular and Epidemiological Characteristics of Carbapenemase-Producing <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Clinical Isolates in Japan

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