Endoplasmic reticulum stress may be involved in insulin resistance and lipid metabolism disorders of the white adipose tissues induced by high-fat diet containing industrial trans-fatty acids

Wanqiu Zhu,1,* Xin Niu,1,* Mingxia Wang,1 Zhao Li,2 Hong-Kun Jiang,3 Chuntao Li,4 Samantha J Caton,5 Yinglong Bai11Department of Maternal and Child Health, School of Public Health, China Medical University, Shenyang, Liaoning, People’s Republic of China; 2Department of Cardiology, The Firs...

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Autores principales:	Zhu W, Niu X, Wang M, Li Z, Jiang HK, Li C, Caton SJ, Bai Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	ER stress elaidic acid obesity insulin resistance dyslipidemia adipocytes Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/943c6dc8751047c0b6d1ff30f5fe3ccb
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spelling	oai:doaj.org-article:943c6dc8751047c0b6d1ff30f5fe3ccb2021-12-02T10:02:33ZEndoplasmic reticulum stress may be involved in insulin resistance and lipid metabolism disorders of the white adipose tissues induced by high-fat diet containing industrial trans-fatty acids1178-7007https://doaj.org/article/943c6dc8751047c0b6d1ff30f5fe3ccb2019-08-01T00:00:00Zhttps://www.dovepress.com/endoplasmic-reticulum-stress-may-be-involved-in-insulin-resistance-and-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Wanqiu Zhu,1,* Xin Niu,1,* Mingxia Wang,1 Zhao Li,2 Hong-Kun Jiang,3 Chuntao Li,4 Samantha J Caton,5 Yinglong Bai11Department of Maternal and Child Health, School of Public Health, China Medical University, Shenyang, Liaoning, People’s Republic of China; 2Department of Cardiology, The First Hospital of China Medical University, Shenyang, Liaoning, People’s Republic of China; 3Department of Pediatrics, The First Hospital of China Medical University, Shenyang, Liaoning, People’s Republic of China; 4Information Center, the First Hospital of China Medical University, Shenyang, Liaoning, People’s Republic of China; 5School of Health and Related Research (ScHARR), Public Health, University of Sheffield, Sheffield, S1 4DA, UKCorrespondence: Yinglong BaiDepartment of Maternal and Child Health, School of Public Health, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang 110122, Liaoning, People’s Republic of ChinaTel+86 243 193 9406Fax +86 243 193 9406Email ylbai@cmu.edu.cn*These authors contributed equally to this workBackground: Consumption of industrially produced trans-fatty acids (iTFAs) can result in alteration to lipid profile and glucose metabolism. Moreover, a diet high in iTFAs could increase the risk of obesity, cardiovascular diseases (CVDs) and type 2 diabetes mellitus. Glucose and lipid metabolism are closely linked in white adipose tissue (WAT), yet the underlying mechanisms of the effect of iTFAs in WAT are poorly understood.Materials and methods: Parameters of glucose homeostasis, lipid profiles and markers of endoplasmic reticulum (ER) stress of WAT were measured in rats maintained on a high-fat diet containing margarine (HFD-M) (n=10) compared to controls maintained on standard chow (n=10) over 16 weeks.Results: Fat mass and body weight was significantly increased in rats maintained on the HFD-M compared to controls (P<0.01). HFD-M rats had increased levels of insulin (INS), homeostasis model assessment of insulin resistance and serum lipid profile was significantly altered. The expression of glucose-regulated protein 78 (GRP78) and the phosphorylation of inositol-requiring enzyme 1-alpha and c-Jun N-terminal kinase (JNK) were significantly increased in subcutaneous and retroperitoneal adipose depots of HFD-M-fed rats. In vitro, wider ER lumens were observed in 100μmol/L elaidic acid (EA)-treated human mature adipocytes. We observed activation of ER stress markers, impaired INS receptor signaling and increased lipogenesis in adipocytes after EA exposure. These effects could be alleviated by inhibiting ER stress in adipocytes in vitro.Conclusion: Collectively these data suggest that ER stress may be involved in INS resistance and lipid metabolism disorders induced by high-fat diet containing iTFAs. These findings suggest that WAT could be regarded as a key target organ for inhibiting ER stress to reverse the impaired INS receptor signaling, alleviate lipid metabolism disorders, and provide a novel approach to prevent and treat INS resistance and dyslipidemia-related chronic diseases such as T2MD and CVDs.Keywords: ER stress, elaidic acid, obesity, insulin resistance, dyslipidemia, adipocytes  Zhu WNiu XWang MLi ZJiang HKLi CCaton SJBai YDove Medical PressarticleER stresselaidic acidobesityinsulin resistancedyslipidemiaadipocytesSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 1625-1638 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	ER stress elaidic acid obesity insulin resistance dyslipidemia adipocytes Specialties of internal medicine RC581-951
spellingShingle	ER stress elaidic acid obesity insulin resistance dyslipidemia adipocytes Specialties of internal medicine RC581-951 Zhu W Niu X Wang M Li Z Jiang HK Li C Caton SJ Bai Y Endoplasmic reticulum stress may be involved in insulin resistance and lipid metabolism disorders of the white adipose tissues induced by high-fat diet containing industrial trans-fatty acids
description	Wanqiu Zhu,1,* Xin Niu,1,* Mingxia Wang,1 Zhao Li,2 Hong-Kun Jiang,3 Chuntao Li,4 Samantha J Caton,5 Yinglong Bai11Department of Maternal and Child Health, School of Public Health, China Medical University, Shenyang, Liaoning, People’s Republic of China; 2Department of Cardiology, The First Hospital of China Medical University, Shenyang, Liaoning, People’s Republic of China; 3Department of Pediatrics, The First Hospital of China Medical University, Shenyang, Liaoning, People’s Republic of China; 4Information Center, the First Hospital of China Medical University, Shenyang, Liaoning, People’s Republic of China; 5School of Health and Related Research (ScHARR), Public Health, University of Sheffield, Sheffield, S1 4DA, UKCorrespondence: Yinglong BaiDepartment of Maternal and Child Health, School of Public Health, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang 110122, Liaoning, People’s Republic of ChinaTel+86 243 193 9406Fax +86 243 193 9406Email ylbai@cmu.edu.cn*These authors contributed equally to this workBackground: Consumption of industrially produced trans-fatty acids (iTFAs) can result in alteration to lipid profile and glucose metabolism. Moreover, a diet high in iTFAs could increase the risk of obesity, cardiovascular diseases (CVDs) and type 2 diabetes mellitus. Glucose and lipid metabolism are closely linked in white adipose tissue (WAT), yet the underlying mechanisms of the effect of iTFAs in WAT are poorly understood.Materials and methods: Parameters of glucose homeostasis, lipid profiles and markers of endoplasmic reticulum (ER) stress of WAT were measured in rats maintained on a high-fat diet containing margarine (HFD-M) (n=10) compared to controls maintained on standard chow (n=10) over 16 weeks.Results: Fat mass and body weight was significantly increased in rats maintained on the HFD-M compared to controls (P<0.01). HFD-M rats had increased levels of insulin (INS), homeostasis model assessment of insulin resistance and serum lipid profile was significantly altered. The expression of glucose-regulated protein 78 (GRP78) and the phosphorylation of inositol-requiring enzyme 1-alpha and c-Jun N-terminal kinase (JNK) were significantly increased in subcutaneous and retroperitoneal adipose depots of HFD-M-fed rats. In vitro, wider ER lumens were observed in 100μmol/L elaidic acid (EA)-treated human mature adipocytes. We observed activation of ER stress markers, impaired INS receptor signaling and increased lipogenesis in adipocytes after EA exposure. These effects could be alleviated by inhibiting ER stress in adipocytes in vitro.Conclusion: Collectively these data suggest that ER stress may be involved in INS resistance and lipid metabolism disorders induced by high-fat diet containing iTFAs. These findings suggest that WAT could be regarded as a key target organ for inhibiting ER stress to reverse the impaired INS receptor signaling, alleviate lipid metabolism disorders, and provide a novel approach to prevent and treat INS resistance and dyslipidemia-related chronic diseases such as T2MD and CVDs.Keywords: ER stress, elaidic acid, obesity, insulin resistance, dyslipidemia, adipocytes
format	article
author	Zhu W Niu X Wang M Li Z Jiang HK Li C Caton SJ Bai Y
author_facet	Zhu W Niu X Wang M Li Z Jiang HK Li C Caton SJ Bai Y
author_sort	Zhu W
title	Endoplasmic reticulum stress may be involved in insulin resistance and lipid metabolism disorders of the white adipose tissues induced by high-fat diet containing industrial trans-fatty acids
title_short	Endoplasmic reticulum stress may be involved in insulin resistance and lipid metabolism disorders of the white adipose tissues induced by high-fat diet containing industrial trans-fatty acids
title_full	Endoplasmic reticulum stress may be involved in insulin resistance and lipid metabolism disorders of the white adipose tissues induced by high-fat diet containing industrial trans-fatty acids
title_fullStr	Endoplasmic reticulum stress may be involved in insulin resistance and lipid metabolism disorders of the white adipose tissues induced by high-fat diet containing industrial trans-fatty acids
title_full_unstemmed	Endoplasmic reticulum stress may be involved in insulin resistance and lipid metabolism disorders of the white adipose tissues induced by high-fat diet containing industrial trans-fatty acids
title_sort	endoplasmic reticulum stress may be involved in insulin resistance and lipid metabolism disorders of the white adipose tissues induced by high-fat diet containing industrial trans-fatty acids
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/943c6dc8751047c0b6d1ff30f5fe3ccb
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Endoplasmic reticulum stress may be involved in insulin resistance and lipid metabolism disorders of the white adipose tissues induced by high-fat diet containing industrial trans-fatty acids

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