Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion

Abstract Cholesterol plays a crucial role in cell membrane organization, dynamics and function. Depletion of cholesterol represents a popular approach to explore cholesterol-sensitivity of membrane proteins. An emerging body of literature shows that the consequence of membrane cholesterol depletion...

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Autores principales:	Parijat Sarkar, Hirak Chakraborty, Amitabha Chattopadhyay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/943d4377df7b48b2b8b35ea5c2f3ffa5
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spelling	oai:doaj.org-article:943d4377df7b48b2b8b35ea5c2f3ffa52021-12-02T15:06:26ZDifferential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion10.1038/s41598-017-04769-42045-2322https://doaj.org/article/943d4377df7b48b2b8b35ea5c2f3ffa52017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04769-4https://doaj.org/toc/2045-2322Abstract Cholesterol plays a crucial role in cell membrane organization, dynamics and function. Depletion of cholesterol represents a popular approach to explore cholesterol-sensitivity of membrane proteins. An emerging body of literature shows that the consequence of membrane cholesterol depletion often depends on the actual process (acute or chronic), although the molecular mechanism underlying the difference is not clear. Acute depletion, using cyclodextrin-type carriers, is faster relative to chronic depletion, in which inhibitors of cholesterol biosynthesis are used. With the overall goal of addressing molecular differences underlying these processes, we monitored membrane dipole potential under conditions of acute and chronic cholesterol depletion in CHO-K1 cells, using a voltage-sensitive fluorescent dye in dual wavelength ratiometric mode. Our results show that the observed membrane dipole potential exhibits difference under acute and chronic cholesterol depletion conditions, even when cholesterol content was identical. To the best of our knowledge, these results provide, for the first time, molecular insight highlighting differences in dipolar reorganization in these processes. A comprehensive understanding of processes in which membrane cholesterol gets modulated would provide novel insight in its interaction with membrane proteins and receptors, thereby allowing us to understand the role of cholesterol in cellular physiology associated with health and disease.Parijat SarkarHirak ChakrabortyAmitabha ChattopadhyayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Parijat Sarkar Hirak Chakraborty Amitabha Chattopadhyay Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion
description	Abstract Cholesterol plays a crucial role in cell membrane organization, dynamics and function. Depletion of cholesterol represents a popular approach to explore cholesterol-sensitivity of membrane proteins. An emerging body of literature shows that the consequence of membrane cholesterol depletion often depends on the actual process (acute or chronic), although the molecular mechanism underlying the difference is not clear. Acute depletion, using cyclodextrin-type carriers, is faster relative to chronic depletion, in which inhibitors of cholesterol biosynthesis are used. With the overall goal of addressing molecular differences underlying these processes, we monitored membrane dipole potential under conditions of acute and chronic cholesterol depletion in CHO-K1 cells, using a voltage-sensitive fluorescent dye in dual wavelength ratiometric mode. Our results show that the observed membrane dipole potential exhibits difference under acute and chronic cholesterol depletion conditions, even when cholesterol content was identical. To the best of our knowledge, these results provide, for the first time, molecular insight highlighting differences in dipolar reorganization in these processes. A comprehensive understanding of processes in which membrane cholesterol gets modulated would provide novel insight in its interaction with membrane proteins and receptors, thereby allowing us to understand the role of cholesterol in cellular physiology associated with health and disease.
format	article
author	Parijat Sarkar Hirak Chakraborty Amitabha Chattopadhyay
author_facet	Parijat Sarkar Hirak Chakraborty Amitabha Chattopadhyay
author_sort	Parijat Sarkar
title	Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion
title_short	Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion
title_full	Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion
title_fullStr	Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion
title_full_unstemmed	Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion
title_sort	differential membrane dipolar orientation induced by acute and chronic cholesterol depletion
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/943d4377df7b48b2b8b35ea5c2f3ffa5
work_keys_str_mv	AT parijatsarkar differentialmembranedipolarorientationinducedbyacuteandchroniccholesteroldepletion AT hirakchakraborty differentialmembranedipolarorientationinducedbyacuteandchroniccholesteroldepletion AT amitabhachattopadhyay differentialmembranedipolarorientationinducedbyacuteandchroniccholesteroldepletion
_version_	1718388464280403968

Differential Membrane Dipolar Orientation Induced by Acute and Chronic Cholesterol Depletion

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