Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.

Lumbar spinal stenosis (LSS), one of the most commonly reported spinal disorders, can cause loss of sensation and dyskinesia. In currently used animal models of LSS, the spinal cord is covered entirely with a silicone sheet, or block-shaped silicone is inserted directly into the spinal canal after l...

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Autores principales:	Hyunseong Kim, Jin Young Hong, Wan-Jin Jeon, Junseon Lee, In-Hyuk Ha
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/943df1110a0b4171940e565fbc998c7f
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spelling	oai:doaj.org-article:943df1110a0b4171940e565fbc998c7f2021-12-02T20:04:06ZEvaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.1932-620310.1371/journal.pone.0251464https://doaj.org/article/943df1110a0b4171940e565fbc998c7f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251464https://doaj.org/toc/1932-6203Lumbar spinal stenosis (LSS), one of the most commonly reported spinal disorders, can cause loss of sensation and dyskinesia. In currently used animal models of LSS, the spinal cord is covered entirely with a silicone sheet, or block-shaped silicone is inserted directly into the spinal canal after laminectomy. However, the effects of differences between these implant materials have not been studied. We assessed the degree of damage and locomotor function of an LSS model in Sprague-Dawley rats using silicone blocks of varying hardness (70, 80, and 90 kPa) implanted at the L4 level. In sham rats, the spinal cord remained intact; in LSS rats, the spinal cord was increasingly compressed by the mechanical pressure of the silicone blocks as hardness increased. Inflammatory cells were not evident in sham rats, but numerous inflammatory cells were observed around the implanted silicone block in LSS rats. CD68+ cell quantification revealed increases in the inflammatory response in a hardness-dependent manner in LSS rats. Compared with those in sham rats, proinflammatory cytokine levels were significantly elevated in a hardness-dependent manner, and locomotor function was significantly decreased, in LSS rats. Overall, this study showed that hardness could be used as an index to control the severity of nerve injury induced by silicone implants.Hyunseong KimJin Young HongWan-Jin JeonJunseon LeeIn-Hyuk HaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251464 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyunseong Kim Jin Young Hong Wan-Jin Jeon Junseon Lee In-Hyuk Ha Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.
description	Lumbar spinal stenosis (LSS), one of the most commonly reported spinal disorders, can cause loss of sensation and dyskinesia. In currently used animal models of LSS, the spinal cord is covered entirely with a silicone sheet, or block-shaped silicone is inserted directly into the spinal canal after laminectomy. However, the effects of differences between these implant materials have not been studied. We assessed the degree of damage and locomotor function of an LSS model in Sprague-Dawley rats using silicone blocks of varying hardness (70, 80, and 90 kPa) implanted at the L4 level. In sham rats, the spinal cord remained intact; in LSS rats, the spinal cord was increasingly compressed by the mechanical pressure of the silicone blocks as hardness increased. Inflammatory cells were not evident in sham rats, but numerous inflammatory cells were observed around the implanted silicone block in LSS rats. CD68+ cell quantification revealed increases in the inflammatory response in a hardness-dependent manner in LSS rats. Compared with those in sham rats, proinflammatory cytokine levels were significantly elevated in a hardness-dependent manner, and locomotor function was significantly decreased, in LSS rats. Overall, this study showed that hardness could be used as an index to control the severity of nerve injury induced by silicone implants.
format	article
author	Hyunseong Kim Jin Young Hong Wan-Jin Jeon Junseon Lee In-Hyuk Ha
author_facet	Hyunseong Kim Jin Young Hong Wan-Jin Jeon Junseon Lee In-Hyuk Ha
author_sort	Hyunseong Kim
title	Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.
title_short	Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.
title_full	Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.
title_fullStr	Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.
title_full_unstemmed	Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.
title_sort	evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/943df1110a0b4171940e565fbc998c7f
work_keys_str_mv	AT hyunseongkim evaluationoftheeffectsofdifferencesinsiliconehardnessonratmodeloflumbarspinalstenosis AT jinyounghong evaluationoftheeffectsofdifferencesinsiliconehardnessonratmodeloflumbarspinalstenosis AT wanjinjeon evaluationoftheeffectsofdifferencesinsiliconehardnessonratmodeloflumbarspinalstenosis AT junseonlee evaluationoftheeffectsofdifferencesinsiliconehardnessonratmodeloflumbarspinalstenosis AT inhyukha evaluationoftheeffectsofdifferencesinsiliconehardnessonratmodeloflumbarspinalstenosis
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Evaluation of the effects of differences in silicone hardness on rat model of lumbar spinal stenosis.

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