Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model

The high costs and extensive time needed for the treatment of critical-sized bone defects are still major clinical concerns in orthopedic surgery; therefore, researchers continue to look for more cost and time-effective methods. This study aims to investigate the effects of a decellularized omentum...

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Autores principales:	Abdulsamet Emet, Erdi Ozdemir, Duygu Uckan Cetinkaya, Emine Kilic, Ramin Hashemihesar, Ali Celalettin Sinan Yuruker, Egemen Turhan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	segmental bone defect PRP omentum scaffold mesenchymal stem cell Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/944117f6ca0d44b2a121572eba80e9cd
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spelling	oai:doaj.org-article:944117f6ca0d44b2a121572eba80e9cd2021-11-25T16:40:10ZEffect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model10.3390/app1122109002076-3417https://doaj.org/article/944117f6ca0d44b2a121572eba80e9cd2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/22/10900https://doaj.org/toc/2076-3417The high costs and extensive time needed for the treatment of critical-sized bone defects are still major clinical concerns in orthopedic surgery; therefore, researchers continue to look for more cost and time-effective methods. This study aims to investigate the effects of a decellularized omentum scaffold with a combination of platelet-rich plasma (PRP) and mesenchymal stem cells on the healing of critical-sized bone defects. Wistar albino rats (<i>n</i> = 30) were investigated in five groups. Critical-sized bone defects were formed on bilateral radius shafts. No scaffold, decellularized omentum, omentum with PRP and omentum + mesenchymal stem cells was used in group 1 (control group), 2, 3 and 4, respectively. In addition, omentum with a combination of mesenchymal stem cells +PRP was used in group 5. After 6 weeks, both radiological and histological healing were evaluated comparatively among the groups. After the use of a decellularized omentum scaffold, vitality of new cells was maintained, and new bone formation occurred. When compared to the control group, radiological healing was significantly better (<i>p</i> = 0.047) in the omentum and omentum + PRP-treated groups. Furthermore, histological healing was better in the omentum and omentum + PRP-treated groups than the control group (<i>p</i> = 0.001). The use of a decellularized omentum scaffold is suitable in the healing of critical bone defects.Abdulsamet EmetErdi OzdemirDuygu Uckan CetinkayaEmine KilicRamin HashemihesarAli Celalettin Sinan YurukerEgemen TurhanMDPI AGarticlesegmental bone defectPRPomentumscaffoldmesenchymal stem cellTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10900, p 10900 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	segmental bone defect PRP omentum scaffold mesenchymal stem cell Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	segmental bone defect PRP omentum scaffold mesenchymal stem cell Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Abdulsamet Emet Erdi Ozdemir Duygu Uckan Cetinkaya Emine Kilic Ramin Hashemihesar Ali Celalettin Sinan Yuruker Egemen Turhan Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model
description	The high costs and extensive time needed for the treatment of critical-sized bone defects are still major clinical concerns in orthopedic surgery; therefore, researchers continue to look for more cost and time-effective methods. This study aims to investigate the effects of a decellularized omentum scaffold with a combination of platelet-rich plasma (PRP) and mesenchymal stem cells on the healing of critical-sized bone defects. Wistar albino rats (<i>n</i> = 30) were investigated in five groups. Critical-sized bone defects were formed on bilateral radius shafts. No scaffold, decellularized omentum, omentum with PRP and omentum + mesenchymal stem cells was used in group 1 (control group), 2, 3 and 4, respectively. In addition, omentum with a combination of mesenchymal stem cells +PRP was used in group 5. After 6 weeks, both radiological and histological healing were evaluated comparatively among the groups. After the use of a decellularized omentum scaffold, vitality of new cells was maintained, and new bone formation occurred. When compared to the control group, radiological healing was significantly better (<i>p</i> = 0.047) in the omentum and omentum + PRP-treated groups. Furthermore, histological healing was better in the omentum and omentum + PRP-treated groups than the control group (<i>p</i> = 0.001). The use of a decellularized omentum scaffold is suitable in the healing of critical bone defects.
format	article
author	Abdulsamet Emet Erdi Ozdemir Duygu Uckan Cetinkaya Emine Kilic Ramin Hashemihesar Ali Celalettin Sinan Yuruker Egemen Turhan
author_facet	Abdulsamet Emet Erdi Ozdemir Duygu Uckan Cetinkaya Emine Kilic Ramin Hashemihesar Ali Celalettin Sinan Yuruker Egemen Turhan
author_sort	Abdulsamet Emet
title	Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model
title_short	Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model
title_full	Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model
title_fullStr	Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model
title_full_unstemmed	Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model
title_sort	effect of a decellularized omentum scaffold with combination of mesenchymal stem cells and platelet-rich plasma on healing of critical-sized bone defect: a rat model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/944117f6ca0d44b2a121572eba80e9cd
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Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model

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