Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast

ABSTRACT The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and pharmaceuticals. The use of this cell factory for cost-efficient production of novel fuels and chemicals requires high yields and low by-product production. Many industrially interes...

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Autor principal:	Jens Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9446ed709f524e6f99e3305807d41c06
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spelling	oai:doaj.org-article:9446ed709f524e6f99e3305807d41c062021-11-15T15:47:04ZSynthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast10.1128/mBio.02153-142150-7511https://doaj.org/article/9446ed709f524e6f99e3305807d41c062014-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02153-14https://doaj.org/toc/2150-7511ABSTRACT The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and pharmaceuticals. The use of this cell factory for cost-efficient production of novel fuels and chemicals requires high yields and low by-product production. Many industrially interesting chemicals are biosynthesized from acetyl coenzyme A (acetyl-CoA), which serves as a central precursor metabolite in yeast. To ensure high yields in production of these chemicals, it is necessary to engineer the central carbon metabolism so that ethanol production is minimized (or eliminated) and acetyl-CoA can be formed from glucose in high yield. Here the perspective of generating yeast platform strains that have such properties is discussed in the context of a major breakthrough with expression of a functional pyruvate dehydrogenase complex in the cytosol.Jens NielsenAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 6 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Jens Nielsen Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast
description	ABSTRACT The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and pharmaceuticals. The use of this cell factory for cost-efficient production of novel fuels and chemicals requires high yields and low by-product production. Many industrially interesting chemicals are biosynthesized from acetyl coenzyme A (acetyl-CoA), which serves as a central precursor metabolite in yeast. To ensure high yields in production of these chemicals, it is necessary to engineer the central carbon metabolism so that ethanol production is minimized (or eliminated) and acetyl-CoA can be formed from glucose in high yield. Here the perspective of generating yeast platform strains that have such properties is discussed in the context of a major breakthrough with expression of a functional pyruvate dehydrogenase complex in the cytosol.
format	article
author	Jens Nielsen
author_facet	Jens Nielsen
author_sort	Jens Nielsen
title	Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast
title_short	Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast
title_full	Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast
title_fullStr	Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast
title_full_unstemmed	Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast
title_sort	synthetic biology for engineering acetyl coenzyme a metabolism in yeast
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/9446ed709f524e6f99e3305807d41c06
work_keys_str_mv	AT jensnielsen syntheticbiologyforengineeringacetylcoenzymeametabolisminyeast
_version_	1718427532931366912

Synthetic Biology for Engineering Acetyl Coenzyme A Metabolism in Yeast

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