FUNCTIONAL ACTIVITY OF IFNα- AND IL-4-INDUCED HUMAN DENDRITIC CELLS: A COMPARATIVE STUDY

We have performed a comparative analysis of allostimulatory, Th1/Th2-stimulatory andtolerogenic activities of dendritic cells (DCs) derived from healthy donors (n = 52). The DCs were generatedin vitro with IFN-alpha (IFN-DCs) or IL-4 (IL-4-DCs). It was shown that IL-4-DCs and IFN-DCs didnot differ i...

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Autores principales:	O. Yu. Leplina, M. A. Tikhonova, T. V. Tyrinova, E. A. Alyamkina, S. S. Bogachev, A. A, Ostanin, E. R. Chernykh
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	dendritic cells allostimulatory activity th1-/th2-сytokines regulatory t-cells Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/9447fe6cf0de493d8b83181ff947ffc5
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spelling	oai:doaj.org-article:9447fe6cf0de493d8b83181ff947ffc52021-11-18T08:03:37ZFUNCTIONAL ACTIVITY OF IFNα- AND IL-4-INDUCED HUMAN DENDRITIC CELLS: A COMPARATIVE STUDY1563-06252313-741X10.15789/1563-0625-2014-1-43-52https://doaj.org/article/9447fe6cf0de493d8b83181ff947ffc52014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/22https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XWe have performed a comparative analysis of allostimulatory, Th1/Th2-stimulatory andtolerogenic activities of dendritic cells (DCs) derived from healthy donors (n = 52). The DCs were generatedin vitro with IFN-alpha (IFN-DCs) or IL-4 (IL-4-DCs). It was shown that IL-4-DCs and IFN-DCs didnot differ in their key functional characteristics, i.e., in their ability to stimulate proliferation of T-cells inresponse to alloantigens, and to induce generation of T-regulatory cells in mixed leukocyte culture. IFN- andIL-4-induced DCs possess similar ability of boosting T cells to produce Th1/proinflammatory (IFNγ, IL-2,IL-1β, TNFα, IL-12, IL-17) and Th2/antiinflammatory cytokines (IL-4, IL-6, IL-10, IL-13), growth factors(G-CSF, GM-CSF, IL-7) and chemokines (IL-8, MIP-1β). Nevertheless, IFN-DCs have more pronouncedstimulatory effect upon Th1 and Th2 cells, thus manifesting as a significantly higher IFNγ, IL-5 and MIP-1βproduction. IFN-DCs were characterized by more prominent ability to activate Th1-cells, and by moderateTh2-stimulatory activity, which is absent in IL-4-DCs. These data strongly suggest that IFN-DCs may presenta quite promising cellular tool for development of therapeutic vaccines aiming to induce/enhance the immuneresponse.O. Yu. LeplinaM. A. TikhonovaT. V. TyrinovaE. A. AlyamkinaS. S. BogachevA. A, OstaninE. R. ChernykhSPb RAACIarticledendritic cellsallostimulatory activityth1-/th2-сytokinesregulatory t-cellsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 16, Iss 1, Pp 43-52 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	dendritic cells allostimulatory activity th1-/th2-сytokines regulatory t-cells Immunologic diseases. Allergy RC581-607
spellingShingle	dendritic cells allostimulatory activity th1-/th2-сytokines regulatory t-cells Immunologic diseases. Allergy RC581-607 O. Yu. Leplina M. A. Tikhonova T. V. Tyrinova E. A. Alyamkina S. S. Bogachev A. A, Ostanin E. R. Chernykh FUNCTIONAL ACTIVITY OF IFNα- AND IL-4-INDUCED HUMAN DENDRITIC CELLS: A COMPARATIVE STUDY
description	We have performed a comparative analysis of allostimulatory, Th1/Th2-stimulatory andtolerogenic activities of dendritic cells (DCs) derived from healthy donors (n = 52). The DCs were generatedin vitro with IFN-alpha (IFN-DCs) or IL-4 (IL-4-DCs). It was shown that IL-4-DCs and IFN-DCs didnot differ in their key functional characteristics, i.e., in their ability to stimulate proliferation of T-cells inresponse to alloantigens, and to induce generation of T-regulatory cells in mixed leukocyte culture. IFN- andIL-4-induced DCs possess similar ability of boosting T cells to produce Th1/proinflammatory (IFNγ, IL-2,IL-1β, TNFα, IL-12, IL-17) and Th2/antiinflammatory cytokines (IL-4, IL-6, IL-10, IL-13), growth factors(G-CSF, GM-CSF, IL-7) and chemokines (IL-8, MIP-1β). Nevertheless, IFN-DCs have more pronouncedstimulatory effect upon Th1 and Th2 cells, thus manifesting as a significantly higher IFNγ, IL-5 and MIP-1βproduction. IFN-DCs were characterized by more prominent ability to activate Th1-cells, and by moderateTh2-stimulatory activity, which is absent in IL-4-DCs. These data strongly suggest that IFN-DCs may presenta quite promising cellular tool for development of therapeutic vaccines aiming to induce/enhance the immuneresponse.
format	article
author	O. Yu. Leplina M. A. Tikhonova T. V. Tyrinova E. A. Alyamkina S. S. Bogachev A. A, Ostanin E. R. Chernykh
author_facet	O. Yu. Leplina M. A. Tikhonova T. V. Tyrinova E. A. Alyamkina S. S. Bogachev A. A, Ostanin E. R. Chernykh
author_sort	O. Yu. Leplina
title	FUNCTIONAL ACTIVITY OF IFNα- AND IL-4-INDUCED HUMAN DENDRITIC CELLS: A COMPARATIVE STUDY
title_short	FUNCTIONAL ACTIVITY OF IFNα- AND IL-4-INDUCED HUMAN DENDRITIC CELLS: A COMPARATIVE STUDY
title_full	FUNCTIONAL ACTIVITY OF IFNα- AND IL-4-INDUCED HUMAN DENDRITIC CELLS: A COMPARATIVE STUDY
title_fullStr	FUNCTIONAL ACTIVITY OF IFNα- AND IL-4-INDUCED HUMAN DENDRITIC CELLS: A COMPARATIVE STUDY
title_full_unstemmed	FUNCTIONAL ACTIVITY OF IFNα- AND IL-4-INDUCED HUMAN DENDRITIC CELLS: A COMPARATIVE STUDY
title_sort	functional activity of ifnα- and il-4-induced human dendritic cells: a comparative study
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/9447fe6cf0de493d8b83181ff947ffc5
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_version_	1718422558919884800

FUNCTIONAL ACTIVITY OF IFNα- AND IL-4-INDUCED HUMAN DENDRITIC CELLS: A COMPARATIVE STUDY

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