The Immunoglobulin Superfamily Member Basigin Is Required for Complex Dendrite Formation in Drosophila

Coordination of dendrite growth with changes in the surrounding substrate occurs widely in the nervous system and is vital for establishing and maintaining neural circuits. However, the molecular basis of this important developmental process remains poorly understood. To identify potential mediators...

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Autores principales:	Brikha R. Shrestha, Anita Burgos, Wesley B. Grueber
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Basigin dendrite morphogenesis Drosophila dendritic arborization neurons sensory neuron dendrite-substrate interaction Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/944d8b51e74b4f4bb1140067640069b1
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spelling	oai:doaj.org-article:944d8b51e74b4f4bb1140067640069b12021-11-04T09:01:18ZThe Immunoglobulin Superfamily Member Basigin Is Required for Complex Dendrite Formation in Drosophila1662-510210.3389/fncel.2021.739741https://doaj.org/article/944d8b51e74b4f4bb1140067640069b12021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fncel.2021.739741/fullhttps://doaj.org/toc/1662-5102Coordination of dendrite growth with changes in the surrounding substrate occurs widely in the nervous system and is vital for establishing and maintaining neural circuits. However, the molecular basis of this important developmental process remains poorly understood. To identify potential mediators of neuron-substrate interactions important for dendrite morphogenesis, we undertook an expression pattern-based screen in Drosophila larvae, which revealed many proteins with expression in dendritic arborization (da) sensory neurons and in neurons and their epidermal substrate. We found that reporters for Basigin, a cell surface molecule of the immunoglobulin (Ig) superfamily previously implicated in cell-cell and cell-substrate interactions, are expressed in da sensory neurons and epidermis. Loss of Basigin in da neurons led to defects in morphogenesis of the complex dendrites of class IV da neurons. Classes of sensory neurons with simpler branching patterns were unaffected by loss of Basigin. Structure-function analyses showed that a juxtamembrane KRR motif is critical for this function. Furthermore, knock down of Basigin in the epidermis led to defects in dendrite elaboration of class IV neurons, suggesting a non-autonomous role. Together, our findings support a role for Basigin in complex dendrite morphogenesis and interactions between dendrites and the adjacent epidermis.Brikha R. ShresthaAnita BurgosWesley B. GrueberWesley B. GrueberWesley B. GrueberFrontiers Media S.A.articleBasigindendrite morphogenesisDrosophiladendritic arborization neuronssensory neurondendrite-substrate interactionNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Cellular Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Basigin dendrite morphogenesis Drosophila dendritic arborization neurons sensory neuron dendrite-substrate interaction Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	Basigin dendrite morphogenesis Drosophila dendritic arborization neurons sensory neuron dendrite-substrate interaction Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Brikha R. Shrestha Anita Burgos Wesley B. Grueber Wesley B. Grueber Wesley B. Grueber The Immunoglobulin Superfamily Member Basigin Is Required for Complex Dendrite Formation in Drosophila
description	Coordination of dendrite growth with changes in the surrounding substrate occurs widely in the nervous system and is vital for establishing and maintaining neural circuits. However, the molecular basis of this important developmental process remains poorly understood. To identify potential mediators of neuron-substrate interactions important for dendrite morphogenesis, we undertook an expression pattern-based screen in Drosophila larvae, which revealed many proteins with expression in dendritic arborization (da) sensory neurons and in neurons and their epidermal substrate. We found that reporters for Basigin, a cell surface molecule of the immunoglobulin (Ig) superfamily previously implicated in cell-cell and cell-substrate interactions, are expressed in da sensory neurons and epidermis. Loss of Basigin in da neurons led to defects in morphogenesis of the complex dendrites of class IV da neurons. Classes of sensory neurons with simpler branching patterns were unaffected by loss of Basigin. Structure-function analyses showed that a juxtamembrane KRR motif is critical for this function. Furthermore, knock down of Basigin in the epidermis led to defects in dendrite elaboration of class IV neurons, suggesting a non-autonomous role. Together, our findings support a role for Basigin in complex dendrite morphogenesis and interactions between dendrites and the adjacent epidermis.
format	article
author	Brikha R. Shrestha Anita Burgos Wesley B. Grueber Wesley B. Grueber Wesley B. Grueber
author_facet	Brikha R. Shrestha Anita Burgos Wesley B. Grueber Wesley B. Grueber Wesley B. Grueber
author_sort	Brikha R. Shrestha
title	The Immunoglobulin Superfamily Member Basigin Is Required for Complex Dendrite Formation in Drosophila
title_short	The Immunoglobulin Superfamily Member Basigin Is Required for Complex Dendrite Formation in Drosophila
title_full	The Immunoglobulin Superfamily Member Basigin Is Required for Complex Dendrite Formation in Drosophila
title_fullStr	The Immunoglobulin Superfamily Member Basigin Is Required for Complex Dendrite Formation in Drosophila
title_full_unstemmed	The Immunoglobulin Superfamily Member Basigin Is Required for Complex Dendrite Formation in Drosophila
title_sort	immunoglobulin superfamily member basigin is required for complex dendrite formation in drosophila
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/944d8b51e74b4f4bb1140067640069b1
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The Immunoglobulin Superfamily Member Basigin Is Required for Complex Dendrite Formation in Drosophila

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