Effective collection of volatile organic compounds in water using rimming flow with odorant-binding proteins

Water solution of volatile organic compounds (VOCs) in air, especially explosive materials, is required to detect VOCs by biosensors efficiently. We developed a collecting device like olfactory mechanism of animals: thin film of water including odorant binding proteins (OBPs), which play a major rol...

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Autores principales:	Hitoshi KIDA, Satoshi TSUKADA, Yoshiyuki TAGAWA, Ryoichi SATO, Masaharu KAMEDA
Formato:	article
Lenguaje:	EN
Publicado:	The Japan Society of Mechanical Engineers 2015
Materias:	dissolution rimming flow volatile organic compounds odorant binding proteins bisosensing Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/9453da8bf8e140f2b11409882d6118c9
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spelling	oai:doaj.org-article:9453da8bf8e140f2b11409882d6118c92021-11-26T06:27:48ZEffective collection of volatile organic compounds in water using rimming flow with odorant-binding proteins2187-974510.1299/mej.15-00244https://doaj.org/article/9453da8bf8e140f2b11409882d6118c92015-07-01T00:00:00Zhttps://www.jstage.jst.go.jp/article/mej/2/4/2_15-00244/_pdf/-char/enhttps://doaj.org/toc/2187-9745Water solution of volatile organic compounds (VOCs) in air, especially explosive materials, is required to detect VOCs by biosensors efficiently. We developed a collecting device like olfactory mechanism of animals: thin film of water including odorant binding proteins (OBPs), which play a major role in transport of hydrophobic odor molecules to olfactory receptors. Thin liquid film was generated using rimming flow on the inner surface of a rotating glass cylinder. Two kinds of OBPs derived from Bombyx mori (Pheromone Binding Protein 1: PBP1, General Odorant Binding Protein 2: GOBP2) were used as additive in pure water. Eugenol was used to simulate an explosive substance for collection. The sample gas including dilute eugenol was collected by the rimming flow device. The concentration of eugenol in solution was measured by gas chromatography. The experimental results showed that the efficiency for the collection of eugenol increased with the revolution rate of the cylinder. Increase in the efficiency was mainly attributed to the spread of the liquid in the cylinder. Furthermore, as the revolution rate was increased, the mole fraction of eugenol near the inside wall was higher than at the axis of rotation by centrifugal force. This might enhance the dissolution of eugenol in water. The collection efficiency further increased by the adding of both proteins and PBP1 exhibited higher affinity for eugenol.Hitoshi KIDASatoshi TSUKADAYoshiyuki TAGAWARyoichi SATOMasaharu KAMEDAThe Japan Society of Mechanical Engineersarticledissolutionrimming flowvolatile organic compoundsodorant binding proteinsbisosensingMechanical engineering and machineryTJ1-1570ENMechanical Engineering Journal, Vol 2, Iss 4, Pp 15-00244-15-00244 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	dissolution rimming flow volatile organic compounds odorant binding proteins bisosensing Mechanical engineering and machinery TJ1-1570
spellingShingle	dissolution rimming flow volatile organic compounds odorant binding proteins bisosensing Mechanical engineering and machinery TJ1-1570 Hitoshi KIDA Satoshi TSUKADA Yoshiyuki TAGAWA Ryoichi SATO Masaharu KAMEDA Effective collection of volatile organic compounds in water using rimming flow with odorant-binding proteins
description	Water solution of volatile organic compounds (VOCs) in air, especially explosive materials, is required to detect VOCs by biosensors efficiently. We developed a collecting device like olfactory mechanism of animals: thin film of water including odorant binding proteins (OBPs), which play a major role in transport of hydrophobic odor molecules to olfactory receptors. Thin liquid film was generated using rimming flow on the inner surface of a rotating glass cylinder. Two kinds of OBPs derived from Bombyx mori (Pheromone Binding Protein 1: PBP1, General Odorant Binding Protein 2: GOBP2) were used as additive in pure water. Eugenol was used to simulate an explosive substance for collection. The sample gas including dilute eugenol was collected by the rimming flow device. The concentration of eugenol in solution was measured by gas chromatography. The experimental results showed that the efficiency for the collection of eugenol increased with the revolution rate of the cylinder. Increase in the efficiency was mainly attributed to the spread of the liquid in the cylinder. Furthermore, as the revolution rate was increased, the mole fraction of eugenol near the inside wall was higher than at the axis of rotation by centrifugal force. This might enhance the dissolution of eugenol in water. The collection efficiency further increased by the adding of both proteins and PBP1 exhibited higher affinity for eugenol.
format	article
author	Hitoshi KIDA Satoshi TSUKADA Yoshiyuki TAGAWA Ryoichi SATO Masaharu KAMEDA
author_facet	Hitoshi KIDA Satoshi TSUKADA Yoshiyuki TAGAWA Ryoichi SATO Masaharu KAMEDA
author_sort	Hitoshi KIDA
title	Effective collection of volatile organic compounds in water using rimming flow with odorant-binding proteins
title_short	Effective collection of volatile organic compounds in water using rimming flow with odorant-binding proteins
title_full	Effective collection of volatile organic compounds in water using rimming flow with odorant-binding proteins
title_fullStr	Effective collection of volatile organic compounds in water using rimming flow with odorant-binding proteins
title_full_unstemmed	Effective collection of volatile organic compounds in water using rimming flow with odorant-binding proteins
title_sort	effective collection of volatile organic compounds in water using rimming flow with odorant-binding proteins
publisher	The Japan Society of Mechanical Engineers
publishDate	2015
url	https://doaj.org/article/9453da8bf8e140f2b11409882d6118c9
work_keys_str_mv	AT hitoshikida effectivecollectionofvolatileorganiccompoundsinwaterusingrimmingflowwithodorantbindingproteins AT satoshitsukada effectivecollectionofvolatileorganiccompoundsinwaterusingrimmingflowwithodorantbindingproteins AT yoshiyukitagawa effectivecollectionofvolatileorganiccompoundsinwaterusingrimmingflowwithodorantbindingproteins AT ryoichisato effectivecollectionofvolatileorganiccompoundsinwaterusingrimmingflowwithodorantbindingproteins AT masaharukameda effectivecollectionofvolatileorganiccompoundsinwaterusingrimmingflowwithodorantbindingproteins
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Effective collection of volatile organic compounds in water using rimming flow with odorant-binding proteins

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