Serological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China.

Paratuberculosis a contagious and chronic disease in domestic and wild ruminants, is caused by Mycobacterium avium subspecies paratuberculosis (MAP). Typical clinical signs include intractable diarrhea, progressive emaciation, proliferative enteropathy, and mesenteric lymphadenitis. Paratuberculosis...

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Autores principales:	Li Zhao, Yu Wang, Jin-Ling Wang, Wei-Hong Zhao, Hui-Xin Cheng, Yi-Min Ma, Hai-Liang Chai, Zhan-Sheng Zhang, Li-Feng Wang, Zeng-Qiang Miao, Yu-Lin Ding, Jirintai Sulijid, Guang-Hui Dang, Shu-Ying Liu, Feng-Long Wang, Si-Guo Liu, Yong-Hong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/945c8a7b7d364d71922155d18bf405ae
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spelling	oai:doaj.org-article:945c8a7b7d364d71922155d18bf405ae2021-12-02T20:08:30ZSerological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China.1932-620310.1371/journal.pone.0256628https://doaj.org/article/945c8a7b7d364d71922155d18bf405ae2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256628https://doaj.org/toc/1932-6203Paratuberculosis a contagious and chronic disease in domestic and wild ruminants, is caused by Mycobacterium avium subspecies paratuberculosis (MAP). Typical clinical signs include intractable diarrhea, progressive emaciation, proliferative enteropathy, and mesenteric lymphadenitis. Paratuberculosis is endemic to many parts of the world and responsible for considerable economic losses. In this study, different types of paratuberculosis and MAP in sheep and goats were investigated in Inner Mongolia, a northern province in China contiguous with two countries and eight other provinces. A total of 4434 serum samples were collected from six cities in the western, central, and eastern regions of Inner Mongolia and analyzed using the ELISA test. In addition, tissue samples were collected from seven animals that were suspected to be infected with MAP. Finally, these tissues samples were analyzed by histopathological examination followed by polymerase chain reaction (PCR), IS1311 PCR-restriction enzyme analysis (PCR-REA), and a sequence analysis of five genes. Among all 4434 ruminant serum samples collected from the six cities in the western, central, and eastern regions of Inner Mongolia, 7.60% (337/4434) measured positive for the MAP antibody. The proportions of positive MAP antibody results for serum samples collected in the western, central, and eastern regions were 5.10% (105/2058), 6.63% (85/1282), and 13.44% (147/1094), respectively. For the seven suspected infected animals selected from the herd with the highest rate of positivity, the gross pathology and histopathology of the necropsied animals were found to be consistent with the pathological features of paratuberculosis. The PCR analysis further confirmed the diagnosis of paratuberculosis. The rest of the results demonstrated that herds of sheep and goats in Inner Mongolia were infected with both MAP type II and type III. To the best of our knowledge, this is the first study of the two subtypes of MAP strains in sheep and goats in Inner Mongolia.Li ZhaoYu WangJin-Ling WangWei-Hong ZhaoHui-Xin ChengYi-Min MaHai-Liang ChaiZhan-Sheng ZhangLi-Feng WangZeng-Qiang MiaoYu-Lin DingJirintai SulijidGuang-Hui DangShu-Ying LiuFeng-Long WangSi-Guo LiuYong-Hong LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256628 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li Zhao Yu Wang Jin-Ling Wang Wei-Hong Zhao Hui-Xin Cheng Yi-Min Ma Hai-Liang Chai Zhan-Sheng Zhang Li-Feng Wang Zeng-Qiang Miao Yu-Lin Ding Jirintai Sulijid Guang-Hui Dang Shu-Ying Liu Feng-Long Wang Si-Guo Liu Yong-Hong Liu Serological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China.
description	Paratuberculosis a contagious and chronic disease in domestic and wild ruminants, is caused by Mycobacterium avium subspecies paratuberculosis (MAP). Typical clinical signs include intractable diarrhea, progressive emaciation, proliferative enteropathy, and mesenteric lymphadenitis. Paratuberculosis is endemic to many parts of the world and responsible for considerable economic losses. In this study, different types of paratuberculosis and MAP in sheep and goats were investigated in Inner Mongolia, a northern province in China contiguous with two countries and eight other provinces. A total of 4434 serum samples were collected from six cities in the western, central, and eastern regions of Inner Mongolia and analyzed using the ELISA test. In addition, tissue samples were collected from seven animals that were suspected to be infected with MAP. Finally, these tissues samples were analyzed by histopathological examination followed by polymerase chain reaction (PCR), IS1311 PCR-restriction enzyme analysis (PCR-REA), and a sequence analysis of five genes. Among all 4434 ruminant serum samples collected from the six cities in the western, central, and eastern regions of Inner Mongolia, 7.60% (337/4434) measured positive for the MAP antibody. The proportions of positive MAP antibody results for serum samples collected in the western, central, and eastern regions were 5.10% (105/2058), 6.63% (85/1282), and 13.44% (147/1094), respectively. For the seven suspected infected animals selected from the herd with the highest rate of positivity, the gross pathology and histopathology of the necropsied animals were found to be consistent with the pathological features of paratuberculosis. The PCR analysis further confirmed the diagnosis of paratuberculosis. The rest of the results demonstrated that herds of sheep and goats in Inner Mongolia were infected with both MAP type II and type III. To the best of our knowledge, this is the first study of the two subtypes of MAP strains in sheep and goats in Inner Mongolia.
format	article
author	Li Zhao Yu Wang Jin-Ling Wang Wei-Hong Zhao Hui-Xin Cheng Yi-Min Ma Hai-Liang Chai Zhan-Sheng Zhang Li-Feng Wang Zeng-Qiang Miao Yu-Lin Ding Jirintai Sulijid Guang-Hui Dang Shu-Ying Liu Feng-Long Wang Si-Guo Liu Yong-Hong Liu
author_facet	Li Zhao Yu Wang Jin-Ling Wang Wei-Hong Zhao Hui-Xin Cheng Yi-Min Ma Hai-Liang Chai Zhan-Sheng Zhang Li-Feng Wang Zeng-Qiang Miao Yu-Lin Ding Jirintai Sulijid Guang-Hui Dang Shu-Ying Liu Feng-Long Wang Si-Guo Liu Yong-Hong Liu
author_sort	Li Zhao
title	Serological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China.
title_short	Serological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China.
title_full	Serological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China.
title_fullStr	Serological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China.
title_full_unstemmed	Serological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China.
title_sort	serological investigation and genotyping of mycobacterium avium subsp. paratuberculosis in sheep and goats in inner mongolia, china.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/945c8a7b7d364d71922155d18bf405ae
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Serological investigation and genotyping of Mycobacterium avium subsp. paratuberculosis in sheep and goats in Inner Mongolia, China.

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