Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection

Riemerella anatipestifer is one of the most devastating pathogens affecting the global duck farms. Infection is involved in secretion of proinflammatory cytokines, including interleukin- (IL-) 17A. During the immune response to infection, IL-22 and IL-17A are often produced concurrently and at high...

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Autores principales:	Rochelle A. Flores, Paula Leona T. Cammayo, Binh T. Nguyen, Cherry P. Fernandez-Colorado, Suk Kim, Woo H. Kim, Wongi Min
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/945cee0d3bd34fcb9771fe0269856262
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spelling	oai:doaj.org-article:945cee0d3bd34fcb9771fe02698562622021-11-22T01:11:20ZDuck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection2314-715610.1155/2021/3862492https://doaj.org/article/945cee0d3bd34fcb9771fe02698562622021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/3862492https://doaj.org/toc/2314-7156Riemerella anatipestifer is one of the most devastating pathogens affecting the global duck farms. Infection is involved in secretion of proinflammatory cytokines, including interleukin- (IL-) 17A. During the immune response to infection, IL-22 and IL-17A are often produced concurrently and at high levels in inflamed tissues. Little is known about duck IL-22 (duIL-22) during R. anatipestifer infection. We describe the characterization of duIL-22 and its mRNA expression analysis in splenic lymphocytes and macrophages treated with heat-killed R. anatipestifer and in the spleens and livers of R. anatipestifer-infected ducks. Full-length cDNA of duIL-22 encoded 197 amino acids. The deduced amino acid sequence of duIL-22 shared a 30.4–40.5% similarity with piscine counterparts, 57.4–60.1% with mammalian homologs, and 93.4% similarity to the chicken. Duck IL-22 mRNA expression level was relatively high in the skin of normal ducks. It was increased in mitogen-stimulated splenic lymphocytes and in killed R. anatipestifer-activated splenic lymphocytes and macrophages. Compared with healthy ducks, IL-22 transcript expression was significantly upregulated in the livers and spleens on days 1 and 4 postinfection, but not on day 7. IL-17A was significantly increased in the spleens only on day 4 postinfection and in the livers at all time points. When splenic lymphocytes were stimulated with heat-killed R. anatipestifer, CD4+ cells predominantly produced IL-22 while IL-17A was expressed both by CD4+ and CD4- cells. These results suggested that IL-22 and IL-17A are likely expressed in different cell types during R. anatipestifer infection.Rochelle A. FloresPaula Leona T. CammayoBinh T. NguyenCherry P. Fernandez-ColoradoSuk KimWoo H. KimWongi MinHindawi LimitedarticleImmunologic diseases. AllergyRC581-607ENJournal of Immunology Research, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607
spellingShingle	Immunologic diseases. Allergy RC581-607 Rochelle A. Flores Paula Leona T. Cammayo Binh T. Nguyen Cherry P. Fernandez-Colorado Suk Kim Woo H. Kim Wongi Min Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection
description	Riemerella anatipestifer is one of the most devastating pathogens affecting the global duck farms. Infection is involved in secretion of proinflammatory cytokines, including interleukin- (IL-) 17A. During the immune response to infection, IL-22 and IL-17A are often produced concurrently and at high levels in inflamed tissues. Little is known about duck IL-22 (duIL-22) during R. anatipestifer infection. We describe the characterization of duIL-22 and its mRNA expression analysis in splenic lymphocytes and macrophages treated with heat-killed R. anatipestifer and in the spleens and livers of R. anatipestifer-infected ducks. Full-length cDNA of duIL-22 encoded 197 amino acids. The deduced amino acid sequence of duIL-22 shared a 30.4–40.5% similarity with piscine counterparts, 57.4–60.1% with mammalian homologs, and 93.4% similarity to the chicken. Duck IL-22 mRNA expression level was relatively high in the skin of normal ducks. It was increased in mitogen-stimulated splenic lymphocytes and in killed R. anatipestifer-activated splenic lymphocytes and macrophages. Compared with healthy ducks, IL-22 transcript expression was significantly upregulated in the livers and spleens on days 1 and 4 postinfection, but not on day 7. IL-17A was significantly increased in the spleens only on day 4 postinfection and in the livers at all time points. When splenic lymphocytes were stimulated with heat-killed R. anatipestifer, CD4+ cells predominantly produced IL-22 while IL-17A was expressed both by CD4+ and CD4- cells. These results suggested that IL-22 and IL-17A are likely expressed in different cell types during R. anatipestifer infection.
format	article
author	Rochelle A. Flores Paula Leona T. Cammayo Binh T. Nguyen Cherry P. Fernandez-Colorado Suk Kim Woo H. Kim Wongi Min
author_facet	Rochelle A. Flores Paula Leona T. Cammayo Binh T. Nguyen Cherry P. Fernandez-Colorado Suk Kim Woo H. Kim Wongi Min
author_sort	Rochelle A. Flores
title	Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection
title_short	Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection
title_full	Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection
title_fullStr	Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection
title_full_unstemmed	Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection
title_sort	duck interleukin-22: identification and expression analysis in riemerella anatipestifer infection
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/945cee0d3bd34fcb9771fe0269856262
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Duck Interleukin-22: Identification and Expression Analysis in Riemerella anatipestifer Infection

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