Development of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology

Diabetic kidney disease (DKD) is a critical complication associated with diabetes; however, there are only a few animal models that can be used to explore its pathogenesis. In the present study, we established a mouse model of DKD using a technique based on the Developmental Origins of Health and Di...

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Autores principales:	Shiori Ishiyama, Mayu Kimura, Takao Nakagawa, Yuka Fujimoto, Kohei Uchimura, Satoshi Kishigami, Kazuki Mochizuki
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	diabetic kidney disease (DKD) MEM mice DOHaD (developmental origins of health and disease) barley glomerulosclerosis transforming growth factor beta (TGF- β) Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/9460dd808d4f465080629a3c1920e000
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spelling	oai:doaj.org-article:9460dd808d4f465080629a3c1920e0002021-11-15T10:57:33ZDevelopment of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology1664-239210.3389/fendo.2021.746838https://doaj.org/article/9460dd808d4f465080629a3c1920e0002021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.746838/fullhttps://doaj.org/toc/1664-2392Diabetic kidney disease (DKD) is a critical complication associated with diabetes; however, there are only a few animal models that can be used to explore its pathogenesis. In the present study, we established a mouse model of DKD using a technique based on the Developmental Origins of Health and Disease theory, i.e., by manipulating the embryonic environment, and investigated whether a dietary intervention could ameliorate the model’s pathology. Two-cell embryos were cultured in vitro in α-minimum essential medium (MEM; MEM mice) or in standard potassium simplex-optimized medium (KSOM) as controls (KSOM mice) for 48 h, and the embryos were reintroduced into the mothers. The MEM and KSOM mice born were fed a high-fat, high-sugar diet for 58 days after they were 8 weeks old. Subsequently, half of the MEM mice and all KSOM mice were fed a diet containing rice powder (control diet), and the remaining MEM mice were fed a diet containing barley powder (barley diet) for 10 weeks. Glomerulosclerosis and pancreatic exhaustion were observed in MEM mice, but not in control KSOM mice. Renal arteriolar changes, including intimal thickening and increase in the rate of hyalinosis, were more pronounced in MEM mice fed a control diet than in KSOM mice. Immunostaining showed the higher expression of transforming growth factor beta (TGFB) in the proximal/distal renal tubules of MEM mice fed a control diet than in those of KSOM mice. Pathologies, such as glomerulosclerosis, renal arteriolar changes, and higher TGFB expression, were ameliorated by barley diet intake in MEM mice. These findings suggested that the MEM mouse is an effective DKD animal model that shows glomerulosclerosis and renal arteriolar changes, and barley intake can improve these pathologies in MEM mice.Shiori IshiyamaMayu KimuraTakao NakagawaYuka FujimotoKohei UchimuraSatoshi KishigamiSatoshi KishigamiKazuki MochizukiKazuki MochizukiFrontiers Media S.A.articlediabetic kidney disease (DKD)MEM miceDOHaD (developmental origins of health and disease)barleyglomerulosclerosistransforming growth factor beta (TGF- β)Diseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetic kidney disease (DKD) MEM mice DOHaD (developmental origins of health and disease) barley glomerulosclerosis transforming growth factor beta (TGF- β) Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	diabetic kidney disease (DKD) MEM mice DOHaD (developmental origins of health and disease) barley glomerulosclerosis transforming growth factor beta (TGF- β) Diseases of the endocrine glands. Clinical endocrinology RC648-665 Shiori Ishiyama Mayu Kimura Takao Nakagawa Yuka Fujimoto Kohei Uchimura Satoshi Kishigami Satoshi Kishigami Kazuki Mochizuki Kazuki Mochizuki Development of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology
description	Diabetic kidney disease (DKD) is a critical complication associated with diabetes; however, there are only a few animal models that can be used to explore its pathogenesis. In the present study, we established a mouse model of DKD using a technique based on the Developmental Origins of Health and Disease theory, i.e., by manipulating the embryonic environment, and investigated whether a dietary intervention could ameliorate the model’s pathology. Two-cell embryos were cultured in vitro in α-minimum essential medium (MEM; MEM mice) or in standard potassium simplex-optimized medium (KSOM) as controls (KSOM mice) for 48 h, and the embryos were reintroduced into the mothers. The MEM and KSOM mice born were fed a high-fat, high-sugar diet for 58 days after they were 8 weeks old. Subsequently, half of the MEM mice and all KSOM mice were fed a diet containing rice powder (control diet), and the remaining MEM mice were fed a diet containing barley powder (barley diet) for 10 weeks. Glomerulosclerosis and pancreatic exhaustion were observed in MEM mice, but not in control KSOM mice. Renal arteriolar changes, including intimal thickening and increase in the rate of hyalinosis, were more pronounced in MEM mice fed a control diet than in KSOM mice. Immunostaining showed the higher expression of transforming growth factor beta (TGFB) in the proximal/distal renal tubules of MEM mice fed a control diet than in those of KSOM mice. Pathologies, such as glomerulosclerosis, renal arteriolar changes, and higher TGFB expression, were ameliorated by barley diet intake in MEM mice. These findings suggested that the MEM mouse is an effective DKD animal model that shows glomerulosclerosis and renal arteriolar changes, and barley intake can improve these pathologies in MEM mice.
format	article
author	Shiori Ishiyama Mayu Kimura Takao Nakagawa Yuka Fujimoto Kohei Uchimura Satoshi Kishigami Satoshi Kishigami Kazuki Mochizuki Kazuki Mochizuki
author_facet	Shiori Ishiyama Mayu Kimura Takao Nakagawa Yuka Fujimoto Kohei Uchimura Satoshi Kishigami Satoshi Kishigami Kazuki Mochizuki Kazuki Mochizuki
author_sort	Shiori Ishiyama
title	Development of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology
title_short	Development of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology
title_full	Development of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology
title_fullStr	Development of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology
title_full_unstemmed	Development of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology
title_sort	development of the diabetic kidney disease mouse model culturing embryos in α-minimum essential medium in vitro, and feeding barley diet attenuated the pathology
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9460dd808d4f465080629a3c1920e000
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Development of the Diabetic Kidney Disease Mouse Model Culturing Embryos in α-Minimum Essential Medium In Vitro, and Feeding Barley Diet Attenuated the Pathology

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