Alkaloid leonurine exerts anti‐inflammatory effects via modulating MST1 expression in trophoblast cells

Abstract Background Pre‐eclampsia (PE) is mainly attributed to the inflammation of trophoblast cells in pregnant women, which results in damage to the maternal organs and growth retardation of the fetus. Alkaloid leonurine (LNR) is a plant compound and has anti‐inflammatory effects. Here we aimed to...

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Autores principales:	Fang Zong, Yingzi Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	leonurine MST1 NF‐κB signal pathway trophoblast cells Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/946471b547eb405cbaa8ae08794c0185
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spelling	oai:doaj.org-article:946471b547eb405cbaa8ae08794c01852021-11-12T19:57:15ZAlkaloid leonurine exerts anti‐inflammatory effects via modulating MST1 expression in trophoblast cells2050-452710.1002/iid3.493https://doaj.org/article/946471b547eb405cbaa8ae08794c01852021-12-01T00:00:00Zhttps://doi.org/10.1002/iid3.493https://doaj.org/toc/2050-4527Abstract Background Pre‐eclampsia (PE) is mainly attributed to the inflammation of trophoblast cells in pregnant women, which results in damage to the maternal organs and growth retardation of the fetus. Alkaloid leonurine (LNR) is a plant compound and has anti‐inflammatory effects. Here we aimed to investigate the effects of LNR on human and mouse trophoblast cells and the underlying mechanisms. Methods The levels of the inflammatory factors in trophoblast cells under lipopolysaccharides (LPS) stimulation were analyzed with ELISA. Western blot was employed to examine the protein expression. Trophoblast cells in Mammalian ste20‐like kinase 1 (MST1−/−) or wild type (WT) mice were isolated to examine the expression of signal molecules in the nuclear factor‐κB (NF‐κB) pathway. Concentration‐dependent activity of NF‐κB was examined. The regulation of LNR and MST1 in MST1−/− trophoblast cells was studied as well. Results Our data showed that LNR exhibited anti‐inflammatory effects and suppressed the NF‐κB signaling by inhibiting LPS‐induced inflammation in trophoblast cells. LNR upregulated the expression of MST1, and the anti‐inflammatory role of LNR was greatly relieved in MST1‐knockout trophoblast cells, although it displayed weak roles in NF‐κB signaling. Conclusion LNR exhibits anti‐inflammatory effects on human and mouse trophoblast cells by upregulating MST1 in the NF‐κB signal pathway.Fang ZongYingzi ZhaoWileyarticleleonurineMST1NF‐κB signal pathwaytrophoblast cellsImmunologic diseases. AllergyRC581-607ENImmunity, Inflammation and Disease, Vol 9, Iss 4, Pp 1439-1446 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	leonurine MST1 NF‐κB signal pathway trophoblast cells Immunologic diseases. Allergy RC581-607
spellingShingle	leonurine MST1 NF‐κB signal pathway trophoblast cells Immunologic diseases. Allergy RC581-607 Fang Zong Yingzi Zhao Alkaloid leonurine exerts anti‐inflammatory effects via modulating MST1 expression in trophoblast cells
description	Abstract Background Pre‐eclampsia (PE) is mainly attributed to the inflammation of trophoblast cells in pregnant women, which results in damage to the maternal organs and growth retardation of the fetus. Alkaloid leonurine (LNR) is a plant compound and has anti‐inflammatory effects. Here we aimed to investigate the effects of LNR on human and mouse trophoblast cells and the underlying mechanisms. Methods The levels of the inflammatory factors in trophoblast cells under lipopolysaccharides (LPS) stimulation were analyzed with ELISA. Western blot was employed to examine the protein expression. Trophoblast cells in Mammalian ste20‐like kinase 1 (MST1−/−) or wild type (WT) mice were isolated to examine the expression of signal molecules in the nuclear factor‐κB (NF‐κB) pathway. Concentration‐dependent activity of NF‐κB was examined. The regulation of LNR and MST1 in MST1−/− trophoblast cells was studied as well. Results Our data showed that LNR exhibited anti‐inflammatory effects and suppressed the NF‐κB signaling by inhibiting LPS‐induced inflammation in trophoblast cells. LNR upregulated the expression of MST1, and the anti‐inflammatory role of LNR was greatly relieved in MST1‐knockout trophoblast cells, although it displayed weak roles in NF‐κB signaling. Conclusion LNR exhibits anti‐inflammatory effects on human and mouse trophoblast cells by upregulating MST1 in the NF‐κB signal pathway.
format	article
author	Fang Zong Yingzi Zhao
author_facet	Fang Zong Yingzi Zhao
author_sort	Fang Zong
title	Alkaloid leonurine exerts anti‐inflammatory effects via modulating MST1 expression in trophoblast cells
title_short	Alkaloid leonurine exerts anti‐inflammatory effects via modulating MST1 expression in trophoblast cells
title_full	Alkaloid leonurine exerts anti‐inflammatory effects via modulating MST1 expression in trophoblast cells
title_fullStr	Alkaloid leonurine exerts anti‐inflammatory effects via modulating MST1 expression in trophoblast cells
title_full_unstemmed	Alkaloid leonurine exerts anti‐inflammatory effects via modulating MST1 expression in trophoblast cells
title_sort	alkaloid leonurine exerts anti‐inflammatory effects via modulating mst1 expression in trophoblast cells
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/946471b547eb405cbaa8ae08794c0185
work_keys_str_mv	AT fangzong alkaloidleonurineexertsantiinflammatoryeffectsviamodulatingmst1expressionintrophoblastcells AT yingzizhao alkaloidleonurineexertsantiinflammatoryeffectsviamodulatingmst1expressionintrophoblastcells
_version_	1718430330575126528

Alkaloid leonurine exerts anti‐inflammatory effects via modulating MST1 expression in trophoblast cells

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