Investigasi resistensi Anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (VGSC)

<p>Extensive and continuous use of pyrethroid insecticides to prevent the transmission of malaria can lead to mutations in the voltage gate sodium channel gene (VGSC) in mosquito vectors. This gene mutation associated with insensitivity pyrethroid is known as knockdown resistance (kdr).<em&...

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Autores principales:	Didid Haryanto, Dalilah Dalilah, Chairil Anwar, Gita Dwi Prasasti, Dwi Handayani, Ahmad Ghiffari
Formato:	article
Lenguaje:	EN ID
Publicado:	The Entomological Society of Indonesia 2019
Materias:	anopheles vagus insektisida piretroid knock down resistance mutasi gen voltage gated sodium channel Zoology QL1-991
Acceso en línea:	https://doaj.org/article/9466de5227c347bdbf3146d04802afac
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spelling	oai:doaj.org-article:9466de5227c347bdbf3146d04802afac2021-12-02T01:04:58ZInvestigasi resistensi Anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (VGSC)1829-77222089-025710.5994/jei.15.3.134https://doaj.org/article/9466de5227c347bdbf3146d04802afac2019-04-01T00:00:00Zhttp://jurnal.pei-pusat.org/index.php/jei/article/view/495https://doaj.org/toc/1829-7722https://doaj.org/toc/2089-0257<p>Extensive and continuous use of pyrethroid insecticides to prevent the transmission of malaria can lead to mutations in the voltage gate sodium channel gene (VGSC) in mosquito vectors. This gene mutation associated with insensitivity pyrethroid is known as knockdown resistance (kdr).<em> </em>The emergence of resistance to pyrethroid insecticides in vector mosquitoes can be a barrier to the successful termination of malaria transmission. Therefore, detection of mutation is necessary to prevent the resistency from build up. The aim of the study was to determine the resistance status of pyrethroid insecticides and identify mutations in the VGSC gene codon 1014 marker for resistance to <em>Anopheles </em>sp. which is a malaria vector in South Sumatra Province. Samples were taken from three districts in South Sumatra: Muara Enim, OKU, and Lahat. The susceptibility enzymatic test of pyrethroid insecticide (permethrin 0.75%) was preliminarily carried out according to WHO 2016 standard on <em>Anopheles vagus </em>Dönitz species. Identification of VGSC gene mutation was performed on all <em>An. vagus </em>that were previously tested for susceptibility and on <em>An. barbirostris </em>van der Wulp species using seminested-PCR and followed by sequencing. The result showed that samples from Muara Enim regency had resistance, whereas in Lahat and OKU regencies were still susceptible to permethrin. From the sequence analysis it is shown that there are no change in DNA kdr bases in VGSC gene insecticide target sites from all regencies. In conclusion, based on molecular studies, there were no pyrethroid insecticide resistance in South Sumatra Province.</p>Didid HaryantoDalilah DalilahChairil AnwarGita Dwi PrasastiDwi HandayaniAhmad GhiffariThe Entomological Society of Indonesiaarticleanopheles vagusinsektisida piretroidknock down resistancemutasi genvoltage gated sodium channelZoologyQL1-991ENIDJurnal Entomologi Indonesia, Vol 15, Iss 3 (2019)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	anopheles vagus insektisida piretroid knock down resistance mutasi gen voltage gated sodium channel Zoology QL1-991
spellingShingle	anopheles vagus insektisida piretroid knock down resistance mutasi gen voltage gated sodium channel Zoology QL1-991 Didid Haryanto Dalilah Dalilah Chairil Anwar Gita Dwi Prasasti Dwi Handayani Ahmad Ghiffari Investigasi resistensi Anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (VGSC)
description	<p>Extensive and continuous use of pyrethroid insecticides to prevent the transmission of malaria can lead to mutations in the voltage gate sodium channel gene (VGSC) in mosquito vectors. This gene mutation associated with insensitivity pyrethroid is known as knockdown resistance (kdr).<em> </em>The emergence of resistance to pyrethroid insecticides in vector mosquitoes can be a barrier to the successful termination of malaria transmission. Therefore, detection of mutation is necessary to prevent the resistency from build up. The aim of the study was to determine the resistance status of pyrethroid insecticides and identify mutations in the VGSC gene codon 1014 marker for resistance to <em>Anopheles </em>sp. which is a malaria vector in South Sumatra Province. Samples were taken from three districts in South Sumatra: Muara Enim, OKU, and Lahat. The susceptibility enzymatic test of pyrethroid insecticide (permethrin 0.75%) was preliminarily carried out according to WHO 2016 standard on <em>Anopheles vagus </em>Dönitz species. Identification of VGSC gene mutation was performed on all <em>An. vagus </em>that were previously tested for susceptibility and on <em>An. barbirostris </em>van der Wulp species using seminested-PCR and followed by sequencing. The result showed that samples from Muara Enim regency had resistance, whereas in Lahat and OKU regencies were still susceptible to permethrin. From the sequence analysis it is shown that there are no change in DNA kdr bases in VGSC gene insecticide target sites from all regencies. In conclusion, based on molecular studies, there were no pyrethroid insecticide resistance in South Sumatra Province.</p>
format	article
author	Didid Haryanto Dalilah Dalilah Chairil Anwar Gita Dwi Prasasti Dwi Handayani Ahmad Ghiffari
author_facet	Didid Haryanto Dalilah Dalilah Chairil Anwar Gita Dwi Prasasti Dwi Handayani Ahmad Ghiffari
author_sort	Didid Haryanto
title	Investigasi resistensi Anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (VGSC)
title_short	Investigasi resistensi Anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (VGSC)
title_full	Investigasi resistensi Anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (VGSC)
title_fullStr	Investigasi resistensi Anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (VGSC)
title_full_unstemmed	Investigasi resistensi Anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (VGSC)
title_sort	investigasi resistensi anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (vgsc)
publisher	The Entomological Society of Indonesia
publishDate	2019
url	https://doaj.org/article/9466de5227c347bdbf3146d04802afac
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Investigasi resistensi Anopheles sp. terhadap insektisida piretroid dan kemungkinan terjadinya mutasi gen voltage gated sodium channel (VGSC)

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