Lamin B1 levels modulate differentiation into neurons during embryonic corticogenesis

Abstract Lamin B1, a key component of the nuclear lamina, plays an important role in brain development. Ablation of endogenous Lamin B1 (Lmnb1) in the mouse strongly impairs embryonic brain development and corticogenesis. However, the mechanisms underlying these neurodevelopmental effects are unknow...

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Autores principales:	Sameehan Mahajani, Caterina Giacomini, Federica Marinaro, Davide De Pietri Tonelli, Andrea Contestabile, Laura Gasparini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/946911ef6d464a83bd2e68edbd01b72d
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spelling	oai:doaj.org-article:946911ef6d464a83bd2e68edbd01b72d2021-12-02T15:05:48ZLamin B1 levels modulate differentiation into neurons during embryonic corticogenesis10.1038/s41598-017-05078-62045-2322https://doaj.org/article/946911ef6d464a83bd2e68edbd01b72d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05078-6https://doaj.org/toc/2045-2322Abstract Lamin B1, a key component of the nuclear lamina, plays an important role in brain development. Ablation of endogenous Lamin B1 (Lmnb1) in the mouse strongly impairs embryonic brain development and corticogenesis. However, the mechanisms underlying these neurodevelopmental effects are unknown. Here, we report that Lamin B1 levels modulate the differentiation of murine neural stem cells (NSCs) into neurons and astroglial-like cells. In vitro, endogenous Lmnb1 depletion favors NSC differentiation into glial fibrillar acidic protein (GFAP)-immunoreactive cells over neurons, while overexpression of human Lamin B1 (LMNB1) increases the proportion of neurons. In Lmnb1-null embryos, neurogenesis is reduced, while in vivo Lmnb1 silencing in mouse embryonic brain by in utero electroporation of a specific Lmnb1 sh-RNA results in aberrant cortical positioning of neurons and increased expression of the astrocytic marker GFAP in the cortex of 7-day old pups. Together, these results indicate that finely tuned levels of Lamin B1 are required for NSC differentiation into neurons, proper expression of the astrocytic marker GFAP and corticogenesis.Sameehan MahajaniCaterina GiacominiFederica MarinaroDavide De Pietri TonelliAndrea ContestabileLaura GaspariniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sameehan Mahajani Caterina Giacomini Federica Marinaro Davide De Pietri Tonelli Andrea Contestabile Laura Gasparini Lamin B1 levels modulate differentiation into neurons during embryonic corticogenesis
description	Abstract Lamin B1, a key component of the nuclear lamina, plays an important role in brain development. Ablation of endogenous Lamin B1 (Lmnb1) in the mouse strongly impairs embryonic brain development and corticogenesis. However, the mechanisms underlying these neurodevelopmental effects are unknown. Here, we report that Lamin B1 levels modulate the differentiation of murine neural stem cells (NSCs) into neurons and astroglial-like cells. In vitro, endogenous Lmnb1 depletion favors NSC differentiation into glial fibrillar acidic protein (GFAP)-immunoreactive cells over neurons, while overexpression of human Lamin B1 (LMNB1) increases the proportion of neurons. In Lmnb1-null embryos, neurogenesis is reduced, while in vivo Lmnb1 silencing in mouse embryonic brain by in utero electroporation of a specific Lmnb1 sh-RNA results in aberrant cortical positioning of neurons and increased expression of the astrocytic marker GFAP in the cortex of 7-day old pups. Together, these results indicate that finely tuned levels of Lamin B1 are required for NSC differentiation into neurons, proper expression of the astrocytic marker GFAP and corticogenesis.
format	article
author	Sameehan Mahajani Caterina Giacomini Federica Marinaro Davide De Pietri Tonelli Andrea Contestabile Laura Gasparini
author_facet	Sameehan Mahajani Caterina Giacomini Federica Marinaro Davide De Pietri Tonelli Andrea Contestabile Laura Gasparini
author_sort	Sameehan Mahajani
title	Lamin B1 levels modulate differentiation into neurons during embryonic corticogenesis
title_short	Lamin B1 levels modulate differentiation into neurons during embryonic corticogenesis
title_full	Lamin B1 levels modulate differentiation into neurons during embryonic corticogenesis
title_fullStr	Lamin B1 levels modulate differentiation into neurons during embryonic corticogenesis
title_full_unstemmed	Lamin B1 levels modulate differentiation into neurons during embryonic corticogenesis
title_sort	lamin b1 levels modulate differentiation into neurons during embryonic corticogenesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/946911ef6d464a83bd2e68edbd01b72d
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Lamin B1 levels modulate differentiation into neurons during embryonic corticogenesis

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