Equilibrium phase diagram and thermal responses of charged DNA-virus rod-suspensions at low ionic strengths

Abstract The collective behavior of DNA is important for exploring new types of bacteria in the means of detection, which is greatly interested in the understanding of interactions between DNAs in living systems. How they self-organize themselves is a physical common phenomenon for broad ranges of t...

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Autor principal:	Kyongok Kang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/946b190a1fa5483eb3fdce27cc1d4989
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spelling	oai:doaj.org-article:946b190a1fa5483eb3fdce27cc1d49892021-12-02T12:09:51ZEquilibrium phase diagram and thermal responses of charged DNA-virus rod-suspensions at low ionic strengths10.1038/s41598-021-82653-y2045-2322https://doaj.org/article/946b190a1fa5483eb3fdce27cc1d49892021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82653-yhttps://doaj.org/toc/2045-2322Abstract The collective behavior of DNA is important for exploring new types of bacteria in the means of detection, which is greatly interested in the understanding of interactions between DNAs in living systems. How they self-organize themselves is a physical common phenomenon for broad ranges of thermodynamic systems. In this work, the equilibrium phase diagrams of charged chiral rods (fd viruses) at low ionic strengths (below a few mM) are provided to demonstrate both replicas of (or self-organized) twist orders and replica symmetry breaking near high concentration glass-states. By varying the ionic strengths, it appears that a critical ionic strength is obtained below 1–2 mM salt, where the twist and freezing of nematic domains diverge. Also, the microscopic relaxation is revealed by the ionic strength-dependent effective Debye screening length. At a fixed low ionic strength, the local orientations of twist are shown by two different length scales of optical pitch, in the chiral-nematic N* phase and the helical domains $$H_{D}$$ H D , for low and high concentration, respectively. RSB occurs in several cases of crossing phase boundary lines in the equilibrium phase diagram of DNA-rod concentration and ionic strength, including long-time kinetic arrests in the presence of twist orders. The different pathways of PATH I, II and III are due to many-body effects of randomized orientations for charged fd rods undergoing long-range electrostatic interactions in bulk elastic medium. In addition, the thermal stability are shown for chiral pitches of the N* phase and the abnormal cooling process of a specific heat in a structural glass. Here, the concentration-driven twist-effects of charged DNA rods are explored using various experimental methods involving image-time correlation, microscopic dynamics in small angle dynamic light scattering, optical activity in second harmonic generation, and differential scanning calorimetry for the glass state.Kyongok KangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kyongok Kang Equilibrium phase diagram and thermal responses of charged DNA-virus rod-suspensions at low ionic strengths
description	Abstract The collective behavior of DNA is important for exploring new types of bacteria in the means of detection, which is greatly interested in the understanding of interactions between DNAs in living systems. How they self-organize themselves is a physical common phenomenon for broad ranges of thermodynamic systems. In this work, the equilibrium phase diagrams of charged chiral rods (fd viruses) at low ionic strengths (below a few mM) are provided to demonstrate both replicas of (or self-organized) twist orders and replica symmetry breaking near high concentration glass-states. By varying the ionic strengths, it appears that a critical ionic strength is obtained below 1–2 mM salt, where the twist and freezing of nematic domains diverge. Also, the microscopic relaxation is revealed by the ionic strength-dependent effective Debye screening length. At a fixed low ionic strength, the local orientations of twist are shown by two different length scales of optical pitch, in the chiral-nematic N* phase and the helical domains $$H_{D}$$ H D , for low and high concentration, respectively. RSB occurs in several cases of crossing phase boundary lines in the equilibrium phase diagram of DNA-rod concentration and ionic strength, including long-time kinetic arrests in the presence of twist orders. The different pathways of PATH I, II and III are due to many-body effects of randomized orientations for charged fd rods undergoing long-range electrostatic interactions in bulk elastic medium. In addition, the thermal stability are shown for chiral pitches of the N* phase and the abnormal cooling process of a specific heat in a structural glass. Here, the concentration-driven twist-effects of charged DNA rods are explored using various experimental methods involving image-time correlation, microscopic dynamics in small angle dynamic light scattering, optical activity in second harmonic generation, and differential scanning calorimetry for the glass state.
format	article
author	Kyongok Kang
author_facet	Kyongok Kang
author_sort	Kyongok Kang
title	Equilibrium phase diagram and thermal responses of charged DNA-virus rod-suspensions at low ionic strengths
title_short	Equilibrium phase diagram and thermal responses of charged DNA-virus rod-suspensions at low ionic strengths
title_full	Equilibrium phase diagram and thermal responses of charged DNA-virus rod-suspensions at low ionic strengths
title_fullStr	Equilibrium phase diagram and thermal responses of charged DNA-virus rod-suspensions at low ionic strengths
title_full_unstemmed	Equilibrium phase diagram and thermal responses of charged DNA-virus rod-suspensions at low ionic strengths
title_sort	equilibrium phase diagram and thermal responses of charged dna-virus rod-suspensions at low ionic strengths
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/946b190a1fa5483eb3fdce27cc1d4989
work_keys_str_mv	AT kyongokkang equilibriumphasediagramandthermalresponsesofchargeddnavirusrodsuspensionsatlowionicstrengths
_version_	1718394642651676672

Equilibrium phase diagram and thermal responses of charged DNA-virus rod-suspensions at low ionic strengths

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