MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis

Abstract Bacterial proteins with MCE domains were first described as being important for Mammalian Cell Entry. More recent evidence suggests they are components of lipid ABC transporters. In Escherichia coli, the single-domain protein MlaD is known to be part of an inner membrane transporter that is...

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Autores principales:	Georgia L. Isom, Nathaniel J. Davies, Zhi-Soon Chong, Jack A. Bryant, Mohammed Jamshad, Maria Sharif, Adam F. Cunningham, Timothy J. Knowles, Shu-Sin Chng, Jeffrey A. Cole, Ian R. Henderson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/946c60bcdf82485e9f3e638e0b3e3962
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spelling	oai:doaj.org-article:946c60bcdf82485e9f3e638e0b3e39622021-12-02T12:30:34ZMCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis10.1038/s41598-017-09111-62045-2322https://doaj.org/article/946c60bcdf82485e9f3e638e0b3e39622017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09111-6https://doaj.org/toc/2045-2322Abstract Bacterial proteins with MCE domains were first described as being important for Mammalian Cell Entry. More recent evidence suggests they are components of lipid ABC transporters. In Escherichia coli, the single-domain protein MlaD is known to be part of an inner membrane transporter that is important for maintenance of outer membrane lipid asymmetry. Here we describe two multi MCE domain-containing proteins in Escherichia coli, PqiB and YebT, the latter of which is an orthologue of MAM-7 that was previously reported to be an outer membrane protein. We show that all three MCE domain-containing proteins localise to the inner membrane. Bioinformatic analyses revealed that MCE domains are widely distributed across bacterial phyla but multi MCE domain-containing proteins evolved in Proteobacteria from single-domain proteins. Mutants defective in mlaD, pqiAB and yebST were shown to have distinct but partially overlapping phenotypes, but the primary functions of PqiB and YebT differ from MlaD. Complementing our previous findings that all three proteins bind phospholipids, results presented here indicate that multi-domain proteins evolved in Proteobacteria for specific functions in maintaining cell envelope homeostasis.Georgia L. IsomNathaniel J. DaviesZhi-Soon ChongJack A. BryantMohammed JamshadMaria SharifAdam F. CunninghamTimothy J. KnowlesShu-Sin ChngJeffrey A. ColeIan R. HendersonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Georgia L. Isom Nathaniel J. Davies Zhi-Soon Chong Jack A. Bryant Mohammed Jamshad Maria Sharif Adam F. Cunningham Timothy J. Knowles Shu-Sin Chng Jeffrey A. Cole Ian R. Henderson MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
description	Abstract Bacterial proteins with MCE domains were first described as being important for Mammalian Cell Entry. More recent evidence suggests they are components of lipid ABC transporters. In Escherichia coli, the single-domain protein MlaD is known to be part of an inner membrane transporter that is important for maintenance of outer membrane lipid asymmetry. Here we describe two multi MCE domain-containing proteins in Escherichia coli, PqiB and YebT, the latter of which is an orthologue of MAM-7 that was previously reported to be an outer membrane protein. We show that all three MCE domain-containing proteins localise to the inner membrane. Bioinformatic analyses revealed that MCE domains are widely distributed across bacterial phyla but multi MCE domain-containing proteins evolved in Proteobacteria from single-domain proteins. Mutants defective in mlaD, pqiAB and yebST were shown to have distinct but partially overlapping phenotypes, but the primary functions of PqiB and YebT differ from MlaD. Complementing our previous findings that all three proteins bind phospholipids, results presented here indicate that multi-domain proteins evolved in Proteobacteria for specific functions in maintaining cell envelope homeostasis.
format	article
author	Georgia L. Isom Nathaniel J. Davies Zhi-Soon Chong Jack A. Bryant Mohammed Jamshad Maria Sharif Adam F. Cunningham Timothy J. Knowles Shu-Sin Chng Jeffrey A. Cole Ian R. Henderson
author_facet	Georgia L. Isom Nathaniel J. Davies Zhi-Soon Chong Jack A. Bryant Mohammed Jamshad Maria Sharif Adam F. Cunningham Timothy J. Knowles Shu-Sin Chng Jeffrey A. Cole Ian R. Henderson
author_sort	Georgia L. Isom
title	MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
title_short	MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
title_full	MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
title_fullStr	MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
title_full_unstemmed	MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
title_sort	mce domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/946c60bcdf82485e9f3e638e0b3e3962
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MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis

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