Effects of Growth Factors and the MicroRNA-183 Family on Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards Auditory Neuron-Like Cells

Gholamreza Farnoosh,1 Mohammad-Reza Mahmoudian-Sani2 1Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; 2Thalassemia and Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCorrespon...

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Autores principales:	Farnoosh G, Mahmoudian-Sani MR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	microrna-183 family auditory neuron human bone marrow-derived mesenchymal stem cell growth factor Cytology QH573-671
Acceso en línea:	https://doaj.org/article/946d88538fba4689aec3e665e3af876c
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spelling	oai:doaj.org-article:946d88538fba4689aec3e665e3af876c2021-12-02T03:35:31ZEffects of Growth Factors and the MicroRNA-183 Family on Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards Auditory Neuron-Like Cells1178-6957https://doaj.org/article/946d88538fba4689aec3e665e3af876c2020-09-01T00:00:00Zhttps://www.dovepress.com/effects-of-growth-factors-and-the-microrna-183-family-on-differentiati-peer-reviewed-article-SCCAAhttps://doaj.org/toc/1178-6957Gholamreza Farnoosh,1 Mohammad-Reza Mahmoudian-Sani2 1Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; 2Thalassemia and Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCorrespondence: Mohammad-Reza Mahmoudian-Sani Tel +98 61-33750410Fax +98 61-33750427Email mohamadsani495@gmail.comIntroduction: Hearing Loss (HL) is known as the most common sensory processing disorder across the world. An effective treatment which has been currently used for patients suffering from this condition is cochlear implant (CI). The major limitation of this treatment is the need for a healthy auditory neuron (AN). Accordingly, mesenchymal cells (MCs) are regarded as good candidates for cell-based therapeutic approaches. The present study aimed to investigate the potentials of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) for differentiation towards ANs along with using treatments with growth factors and microRNA (miRNA) transfection in vitro.Methods: To this end, neurospheres derived from hBM-MSCs were treated via basic fibroblast growth factor (bFGF), neurotrophin-3 (NT-3), and brain-derived neurotrophic factor (BDNF) as growth factors N2 and B27 supplements, as well as miRNA-96, -182, -183 transfected into hBM-MSCs in order to evaluate the differentiation of such cells into ANs.Results: Treatments with growth factors demonstrated a significant increase in neurogenin 1 (Ngn1) and sex determining region Y-box 2 (SOX2) markers; but tubulin, microtubule-associated protein 2 (MAP2), and GATA binding protein 3 (GATA3) markers were not statistically significant. The findings also revealed that miRNA-182 expression in miRNA-183 family could boost the expressions of some AN marker (ie, Ngn1, SOX2, peripherin, and nestin) in vitro.Discussion: It can be concluded that miRNA is probably a good substitute for growth factors used in differentiating into ANs. Transdifferentiation of hBM-MSCs into ANs, which does not occur under normal conditions, may be thus facilitated by miRNAs, especially miRNA-182, or via a combination of miRNA and growth factors.Keywords: microRNA-183 family, auditory neuron, human bone marrow-derived mesenchymal stem cell, growth factorFarnoosh GMahmoudian-Sani MRDove Medical Pressarticlemicrorna-183 familyauditory neuronhuman bone marrow-derived mesenchymal stem cellgrowth factorCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol Volume 13, Pp 79-89 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	microrna-183 family auditory neuron human bone marrow-derived mesenchymal stem cell growth factor Cytology QH573-671
spellingShingle	microrna-183 family auditory neuron human bone marrow-derived mesenchymal stem cell growth factor Cytology QH573-671 Farnoosh G Mahmoudian-Sani MR Effects of Growth Factors and the MicroRNA-183 Family on Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards Auditory Neuron-Like Cells
description	Gholamreza Farnoosh,1 Mohammad-Reza Mahmoudian-Sani2 1Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; 2Thalassemia and Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCorrespondence: Mohammad-Reza Mahmoudian-Sani Tel +98 61-33750410Fax +98 61-33750427Email mohamadsani495@gmail.comIntroduction: Hearing Loss (HL) is known as the most common sensory processing disorder across the world. An effective treatment which has been currently used for patients suffering from this condition is cochlear implant (CI). The major limitation of this treatment is the need for a healthy auditory neuron (AN). Accordingly, mesenchymal cells (MCs) are regarded as good candidates for cell-based therapeutic approaches. The present study aimed to investigate the potentials of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) for differentiation towards ANs along with using treatments with growth factors and microRNA (miRNA) transfection in vitro.Methods: To this end, neurospheres derived from hBM-MSCs were treated via basic fibroblast growth factor (bFGF), neurotrophin-3 (NT-3), and brain-derived neurotrophic factor (BDNF) as growth factors N2 and B27 supplements, as well as miRNA-96, -182, -183 transfected into hBM-MSCs in order to evaluate the differentiation of such cells into ANs.Results: Treatments with growth factors demonstrated a significant increase in neurogenin 1 (Ngn1) and sex determining region Y-box 2 (SOX2) markers; but tubulin, microtubule-associated protein 2 (MAP2), and GATA binding protein 3 (GATA3) markers were not statistically significant. The findings also revealed that miRNA-182 expression in miRNA-183 family could boost the expressions of some AN marker (ie, Ngn1, SOX2, peripherin, and nestin) in vitro.Discussion: It can be concluded that miRNA is probably a good substitute for growth factors used in differentiating into ANs. Transdifferentiation of hBM-MSCs into ANs, which does not occur under normal conditions, may be thus facilitated by miRNAs, especially miRNA-182, or via a combination of miRNA and growth factors.Keywords: microRNA-183 family, auditory neuron, human bone marrow-derived mesenchymal stem cell, growth factor
format	article
author	Farnoosh G Mahmoudian-Sani MR
author_facet	Farnoosh G Mahmoudian-Sani MR
author_sort	Farnoosh G
title	Effects of Growth Factors and the MicroRNA-183 Family on Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards Auditory Neuron-Like Cells
title_short	Effects of Growth Factors and the MicroRNA-183 Family on Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards Auditory Neuron-Like Cells
title_full	Effects of Growth Factors and the MicroRNA-183 Family on Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards Auditory Neuron-Like Cells
title_fullStr	Effects of Growth Factors and the MicroRNA-183 Family on Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards Auditory Neuron-Like Cells
title_full_unstemmed	Effects of Growth Factors and the MicroRNA-183 Family on Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards Auditory Neuron-Like Cells
title_sort	effects of growth factors and the microrna-183 family on differentiation of human bone marrow-derived mesenchymal stem cells towards auditory neuron-like cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/946d88538fba4689aec3e665e3af876c
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Effects of Growth Factors and the MicroRNA-183 Family on Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards Auditory Neuron-Like Cells

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