Uptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated N-trimethyl chitosan nanoparticles across intestinal Caco-2 cell monolayers

Min Guan1, Qiao-Ling Zhu1, Yang Liu1, Yong-Yan Bei1, Zong-Lin Gu1, Xue-Nong Zhang1, Qiang Zhang21Department of Pharmaceutics, College of Pharmaceutical Science, Soochow University, Suzhou, People&#39;s Republic of China; 2Department of Pharmaceutics, School of Pharmaceutical Science, Peking...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang Q, Zhang XN, Gu ZL, Bei YY, Liu Y, Zhu QL, Guan M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9471b7134f5b48b6859ddd052d77028c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9471b7134f5b48b6859ddd052d77028c
record_format	dspace
spelling	oai:doaj.org-article:9471b7134f5b48b6859ddd052d77028c2021-12-02T04:59:18ZUptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated N-trimethyl chitosan nanoparticles across intestinal Caco-2 cell monolayers1176-91141178-2013https://doaj.org/article/9471b7134f5b48b6859ddd052d77028c2012-04-01T00:00:00Zhttp://www.dovepress.com/uptake-and-transport-of-a-novel-anticancer-drug-delivery-system-lactos-a9670https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Min Guan1, Qiao-Ling Zhu1, Yang Liu1, Yong-Yan Bei1, Zong-Lin Gu1, Xue-Nong Zhang1, Qiang Zhang21Department of Pharmaceutics, College of Pharmaceutical Science, Soochow University, Suzhou, People&#39;s Republic of China; 2Department of Pharmaceutics, School of Pharmaceutical Science, Peking University, Beijing, People&#39;s Republic of ChinaAbstract: In this paper, novel liver-targeting nanoparticles (NPs), lactosyl-norcantharidin (Lac-NCTD)-associated N-trimethyl chitosan (TMC) NPs (Lac-NCTD-TMC-NPs), were prepared using ionic cross-linkage. The physical properties, particle size, and encapsulation efficiency of the nanoparticles were then investigated. The continuous line of heterogeneous human epithelial colorectal adenocarcinoma cells (Caco-2) cell monolayer model was used to study the transport mechanism of Lac-NCTD, and the effects of factors such as time, temperature, pH level, drug concentration, enhancers, and inhibitors. This model was also used to indicate the differences among Lac-NCTD, Lac-NCTD-associated chitosan NPs (Lac-NCTD-CS-NPs), and Lac-NCTD-TMC-NPs in the absorption and transportation of membranes. Drug concentration levels were measured using high-performance liquid chromatography. Active transport and paracellular transport were suggested to be both the primary and secondary mechanisms for Lac-NCTD absorption, respectively. Lac-NCTD uptake and absorption were not controlled by pH levels, but were positively correlated to uptake time, and negatively correlated to temperature. The basolateral to apical apparent permeability coefficients (Papps) were higher than those of the apical to basolateral values. The inhibitor of P-glycoprotein and the multidrug resistance-associated protein 2 significantly enhanced the uptake amount of Lac-NCTD. Compared with Lac-NCTD, Lac-NCTD-CS-NPs and Lac-NCTD-TMC-NPs significantly enhanced drug absorption. Additionally, the latter exhibited stronger action. Lac-NCTD-NPs could penetrate the plasma membrane of Caco-2 cells and translocate into the cytoplasm and even into the nucleus. Nanoparticles were uptaken into Caco-2 cells through the endocytosis pathway.Keywords: Lac-NCTD, Lac-NCTD-TMC-NPs, Caco-2 cell, transportZhang QZhang XNGu ZLBei YYLiu YZhu QLGuan MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1921-1930 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang Q Zhang XN Gu ZL Bei YY Liu Y Zhu QL Guan M Uptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated N-trimethyl chitosan nanoparticles across intestinal Caco-2 cell monolayers
description	Min Guan1, Qiao-Ling Zhu1, Yang Liu1, Yong-Yan Bei1, Zong-Lin Gu1, Xue-Nong Zhang1, Qiang Zhang21Department of Pharmaceutics, College of Pharmaceutical Science, Soochow University, Suzhou, People&#39;s Republic of China; 2Department of Pharmaceutics, School of Pharmaceutical Science, Peking University, Beijing, People&#39;s Republic of ChinaAbstract: In this paper, novel liver-targeting nanoparticles (NPs), lactosyl-norcantharidin (Lac-NCTD)-associated N-trimethyl chitosan (TMC) NPs (Lac-NCTD-TMC-NPs), were prepared using ionic cross-linkage. The physical properties, particle size, and encapsulation efficiency of the nanoparticles were then investigated. The continuous line of heterogeneous human epithelial colorectal adenocarcinoma cells (Caco-2) cell monolayer model was used to study the transport mechanism of Lac-NCTD, and the effects of factors such as time, temperature, pH level, drug concentration, enhancers, and inhibitors. This model was also used to indicate the differences among Lac-NCTD, Lac-NCTD-associated chitosan NPs (Lac-NCTD-CS-NPs), and Lac-NCTD-TMC-NPs in the absorption and transportation of membranes. Drug concentration levels were measured using high-performance liquid chromatography. Active transport and paracellular transport were suggested to be both the primary and secondary mechanisms for Lac-NCTD absorption, respectively. Lac-NCTD uptake and absorption were not controlled by pH levels, but were positively correlated to uptake time, and negatively correlated to temperature. The basolateral to apical apparent permeability coefficients (Papps) were higher than those of the apical to basolateral values. The inhibitor of P-glycoprotein and the multidrug resistance-associated protein 2 significantly enhanced the uptake amount of Lac-NCTD. Compared with Lac-NCTD, Lac-NCTD-CS-NPs and Lac-NCTD-TMC-NPs significantly enhanced drug absorption. Additionally, the latter exhibited stronger action. Lac-NCTD-NPs could penetrate the plasma membrane of Caco-2 cells and translocate into the cytoplasm and even into the nucleus. Nanoparticles were uptaken into Caco-2 cells through the endocytosis pathway.Keywords: Lac-NCTD, Lac-NCTD-TMC-NPs, Caco-2 cell, transport
format	article
author	Zhang Q Zhang XN Gu ZL Bei YY Liu Y Zhu QL Guan M
author_facet	Zhang Q Zhang XN Gu ZL Bei YY Liu Y Zhu QL Guan M
author_sort	Zhang Q
title	Uptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated N-trimethyl chitosan nanoparticles across intestinal Caco-2 cell monolayers
title_short	Uptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated N-trimethyl chitosan nanoparticles across intestinal Caco-2 cell monolayers
title_full	Uptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated N-trimethyl chitosan nanoparticles across intestinal Caco-2 cell monolayers
title_fullStr	Uptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated N-trimethyl chitosan nanoparticles across intestinal Caco-2 cell monolayers
title_full_unstemmed	Uptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated N-trimethyl chitosan nanoparticles across intestinal Caco-2 cell monolayers
title_sort	uptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated n-trimethyl chitosan nanoparticles across intestinal caco-2 cell monolayers
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/9471b7134f5b48b6859ddd052d77028c
work_keys_str_mv	AT zhangq uptakeandtransportofanovelanticancerdrugdeliverysystemlactosylnorcantharidinassociatedntrimethylchitosannanoparticlesacrossintestinalcaco2cellmonolayers AT zhangxn uptakeandtransportofanovelanticancerdrugdeliverysystemlactosylnorcantharidinassociatedntrimethylchitosannanoparticlesacrossintestinalcaco2cellmonolayers AT guzl uptakeandtransportofanovelanticancerdrugdeliverysystemlactosylnorcantharidinassociatedntrimethylchitosannanoparticlesacrossintestinalcaco2cellmonolayers AT beiyy uptakeandtransportofanovelanticancerdrugdeliverysystemlactosylnorcantharidinassociatedntrimethylchitosannanoparticlesacrossintestinalcaco2cellmonolayers AT liuy uptakeandtransportofanovelanticancerdrugdeliverysystemlactosylnorcantharidinassociatedntrimethylchitosannanoparticlesacrossintestinalcaco2cellmonolayers AT zhuql uptakeandtransportofanovelanticancerdrugdeliverysystemlactosylnorcantharidinassociatedntrimethylchitosannanoparticlesacrossintestinalcaco2cellmonolayers AT guanm uptakeandtransportofanovelanticancerdrugdeliverysystemlactosylnorcantharidinassociatedntrimethylchitosannanoparticlesacrossintestinalcaco2cellmonolayers
_version_	1718400903697924096

Uptake and transport of a novel anticancer drug-delivery system: lactosyl-norcantharidin-associated N-trimethyl chitosan nanoparticles across intestinal Caco-2 cell monolayers

Ejemplares similares