Chemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity

Abstract Climate change causes permafrost thawing, and we are confronted with the unpredictable risk of newly discovered permafrost microbes that have disease-causing capabilities. Here, we first characterized the detailed chemical structure of the lipid A moiety from a Pseudomonas species that was...

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Autores principales:	Han-Gyu Park, Ganesan Sathiyanarayanan, Cheol-Hwan Hwang, Da-Hee Ann, Jung-Ho Kim, Geul Bang, Kyoung-Soon Jang, Hee Wook Ryu, Yoo Kyung Lee, Yung-Hun Yang, Yun-Gon Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/94819439ba5449b6bee32cfc6c9ae7a4
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spelling	oai:doaj.org-article:94819439ba5449b6bee32cfc6c9ae7a42021-12-02T11:52:38ZChemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity10.1038/s41598-017-02145-w2045-2322https://doaj.org/article/94819439ba5449b6bee32cfc6c9ae7a42017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02145-whttps://doaj.org/toc/2045-2322Abstract Climate change causes permafrost thawing, and we are confronted with the unpredictable risk of newly discovered permafrost microbes that have disease-causing capabilities. Here, we first characterized the detailed chemical structure of the lipid A moiety from a Pseudomonas species that was isolated from thawing arctic permafrost using MALDI-based mass spectrometric approaches (i.e., MALDI-TOF MS and MALDI-QIT-TOF MSn). The MALDI multi-stage mass spectrometry (MS) analysis of lipid A extracted from the Pseudomonas sp. strain PAMC 28618 demonstrated that the hexaacyl lipid A ([M−H]− at m/z 1616.5) contains a glucosamine (GlcN) disaccharide backbone, two phosphates, four main acyl chains and two branched acyl chains. Moreover, the lipid A molecule–based structural activity relationship with other terrestrial Gram-negative bacteria indicated that strain PAMC 28618 has an identical lipid A structure with the mesophilic Pseudomonas cichorii which can cause rot disease in endive (Cichorium endivia) and that their bacterial toxicities were equivalent. Therefore, the overall lipid A validation process provides a general strategy for characterizing bacteria that have been isolated from arctic permafrost and analyzing their respective pathogenicities.Han-Gyu ParkGanesan SathiyanarayananCheol-Hwan HwangDa-Hee AnnJung-Ho KimGeul BangKyoung-Soon JangHee Wook RyuYoo Kyung LeeYung-Hun YangYun-Gon KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Han-Gyu Park Ganesan Sathiyanarayanan Cheol-Hwan Hwang Da-Hee Ann Jung-Ho Kim Geul Bang Kyoung-Soon Jang Hee Wook Ryu Yoo Kyung Lee Yung-Hun Yang Yun-Gon Kim Chemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity
description	Abstract Climate change causes permafrost thawing, and we are confronted with the unpredictable risk of newly discovered permafrost microbes that have disease-causing capabilities. Here, we first characterized the detailed chemical structure of the lipid A moiety from a Pseudomonas species that was isolated from thawing arctic permafrost using MALDI-based mass spectrometric approaches (i.e., MALDI-TOF MS and MALDI-QIT-TOF MSn). The MALDI multi-stage mass spectrometry (MS) analysis of lipid A extracted from the Pseudomonas sp. strain PAMC 28618 demonstrated that the hexaacyl lipid A ([M−H]− at m/z 1616.5) contains a glucosamine (GlcN) disaccharide backbone, two phosphates, four main acyl chains and two branched acyl chains. Moreover, the lipid A molecule–based structural activity relationship with other terrestrial Gram-negative bacteria indicated that strain PAMC 28618 has an identical lipid A structure with the mesophilic Pseudomonas cichorii which can cause rot disease in endive (Cichorium endivia) and that their bacterial toxicities were equivalent. Therefore, the overall lipid A validation process provides a general strategy for characterizing bacteria that have been isolated from arctic permafrost and analyzing their respective pathogenicities.
format	article
author	Han-Gyu Park Ganesan Sathiyanarayanan Cheol-Hwan Hwang Da-Hee Ann Jung-Ho Kim Geul Bang Kyoung-Soon Jang Hee Wook Ryu Yoo Kyung Lee Yung-Hun Yang Yun-Gon Kim
author_facet	Han-Gyu Park Ganesan Sathiyanarayanan Cheol-Hwan Hwang Da-Hee Ann Jung-Ho Kim Geul Bang Kyoung-Soon Jang Hee Wook Ryu Yoo Kyung Lee Yung-Hun Yang Yun-Gon Kim
author_sort	Han-Gyu Park
title	Chemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity
title_short	Chemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity
title_full	Chemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity
title_fullStr	Chemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity
title_full_unstemmed	Chemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity
title_sort	chemical structure of the lipid a component of pseudomonas sp. strain pamc 28618 from thawing permafrost in relation to pathogenicity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/94819439ba5449b6bee32cfc6c9ae7a4
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Chemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity

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