Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology

Abstract Euglenids represent a group of protists with diverse modes of feeding. To date, only a partial genomic sequence of Euglena gracilis and transcriptomes of several phototrophic and secondarily osmotrophic species are available, while primarily heterotrophic euglenids are seriously undersample...

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Autores principales:	Petr Soukal, Štěpánka Hrdá, Anna Karnkowska, Rafał Milanowski, Jana Szabová, Miluše Hradilová, Hynek Strnad, Čestmír Vlček, Ivan Čepička, Vladimír Hampl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9485f77f444f443da288a4ac83180e3a
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spelling	oai:doaj.org-article:9485f77f444f443da288a4ac83180e3a2021-12-02T16:06:09ZHeterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology10.1038/s41598-021-92174-32045-2322https://doaj.org/article/9485f77f444f443da288a4ac83180e3a2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92174-3https://doaj.org/toc/2045-2322Abstract Euglenids represent a group of protists with diverse modes of feeding. To date, only a partial genomic sequence of Euglena gracilis and transcriptomes of several phototrophic and secondarily osmotrophic species are available, while primarily heterotrophic euglenids are seriously undersampled. In this work, we begin to fill this gap by presenting genomic and transcriptomic drafts of a primary osmotroph, Rhabdomonas costata. The current genomic assembly length of 100 Mbp is 14× smaller than that of E. gracilis. Despite being too fragmented for comprehensive gene prediction it provided fragments of the mitochondrial genome and comparison of the transcriptomic and genomic data revealed features of its introns, including several candidates for nonconventional types. A set of 39,456 putative R. costata proteins was predicted from the transcriptome. Annotation of the mitochondrial core metabolism provides the first data on the facultatively anaerobic mitochondrion of R. costata, which in most respects resembles the mitochondrion of E. gracilis with a certain level of streamlining. R. costata can synthetise thiamine by enzymes of heterogenous provenances and haem by a mitochondrial-cytoplasmic C4 pathway with enzymes orthologous to those found in E. gracilis. The low percentage of green algae-affiliated genes supports the ancestrally osmotrophic status of this species.Petr SoukalŠtěpánka HrdáAnna KarnkowskaRafał MilanowskiJana SzabováMiluše HradilováHynek StrnadČestmír VlčekIvan ČepičkaVladimír HamplNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Petr Soukal Štěpánka Hrdá Anna Karnkowska Rafał Milanowski Jana Szabová Miluše Hradilová Hynek Strnad Čestmír Vlček Ivan Čepička Vladimír Hampl Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology
description	Abstract Euglenids represent a group of protists with diverse modes of feeding. To date, only a partial genomic sequence of Euglena gracilis and transcriptomes of several phototrophic and secondarily osmotrophic species are available, while primarily heterotrophic euglenids are seriously undersampled. In this work, we begin to fill this gap by presenting genomic and transcriptomic drafts of a primary osmotroph, Rhabdomonas costata. The current genomic assembly length of 100 Mbp is 14× smaller than that of E. gracilis. Despite being too fragmented for comprehensive gene prediction it provided fragments of the mitochondrial genome and comparison of the transcriptomic and genomic data revealed features of its introns, including several candidates for nonconventional types. A set of 39,456 putative R. costata proteins was predicted from the transcriptome. Annotation of the mitochondrial core metabolism provides the first data on the facultatively anaerobic mitochondrion of R. costata, which in most respects resembles the mitochondrion of E. gracilis with a certain level of streamlining. R. costata can synthetise thiamine by enzymes of heterogenous provenances and haem by a mitochondrial-cytoplasmic C4 pathway with enzymes orthologous to those found in E. gracilis. The low percentage of green algae-affiliated genes supports the ancestrally osmotrophic status of this species.
format	article
author	Petr Soukal Štěpánka Hrdá Anna Karnkowska Rafał Milanowski Jana Szabová Miluše Hradilová Hynek Strnad Čestmír Vlček Ivan Čepička Vladimír Hampl
author_facet	Petr Soukal Štěpánka Hrdá Anna Karnkowska Rafał Milanowski Jana Szabová Miluše Hradilová Hynek Strnad Čestmír Vlček Ivan Čepička Vladimír Hampl
author_sort	Petr Soukal
title	Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology
title_short	Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology
title_full	Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology
title_fullStr	Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology
title_full_unstemmed	Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology
title_sort	heterotrophic euglenid rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9485f77f444f443da288a4ac83180e3a
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Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology

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