Histology, immunohistochemistry, and in situ hybridization reveal overlooked Ebola virus target tissues in the Ebola virus disease guinea pig model

Abstract Survivors of Ebola virus infection may become subclinically infected, but whether animal models recapitulate this complication is unclear. Using histology in combination with immunohistochemistry and in situ hybridization in a retrospective review of a guinea pig confirmation-of-virulence s...

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Autores principales:	Timothy K. Cooper, Louis Huzella, Joshua C. Johnson, Oscar Rojas, Sri Yellayi, Mei G. Sun, Sina Bavari, Amanda Bonilla, Randy Hart, Peter B. Jahrling, Jens H. Kuhn, Xiankun Zeng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/948a329586ed44359c28050be99073b3
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spelling	oai:doaj.org-article:948a329586ed44359c28050be99073b32021-12-02T15:07:45ZHistology, immunohistochemistry, and in situ hybridization reveal overlooked Ebola virus target tissues in the Ebola virus disease guinea pig model10.1038/s41598-018-19638-x2045-2322https://doaj.org/article/948a329586ed44359c28050be99073b32018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19638-xhttps://doaj.org/toc/2045-2322Abstract Survivors of Ebola virus infection may become subclinically infected, but whether animal models recapitulate this complication is unclear. Using histology in combination with immunohistochemistry and in situ hybridization in a retrospective review of a guinea pig confirmation-of-virulence study, we demonstrate for the first time Ebola virus infection in hepatic oval cells, the endocardium and stroma of the atrioventricular valves and chordae tendinae, satellite cells of peripheral ganglia, neurofibroblasts and Schwann cells of peripheral nerves and ganglia, smooth muscle cells of the uterine myometrium and vaginal wall, acini of the parotid salivary glands, thyroid follicular cells, adrenal medullary cells, pancreatic islet cells, endometrial glandular and surface epithelium, and the epithelium of the vagina, penis and, prepuce. These findings indicate that standard animal models for Ebola virus disease are not as well-described as previously thought and may serve as a stepping stone for future identification of potential sites of virus persistence.Timothy K. CooperLouis HuzellaJoshua C. JohnsonOscar RojasSri YellayiMei G. SunSina BavariAmanda BonillaRandy HartPeter B. JahrlingJens H. KuhnXiankun ZengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Timothy K. Cooper Louis Huzella Joshua C. Johnson Oscar Rojas Sri Yellayi Mei G. Sun Sina Bavari Amanda Bonilla Randy Hart Peter B. Jahrling Jens H. Kuhn Xiankun Zeng Histology, immunohistochemistry, and in situ hybridization reveal overlooked Ebola virus target tissues in the Ebola virus disease guinea pig model
description	Abstract Survivors of Ebola virus infection may become subclinically infected, but whether animal models recapitulate this complication is unclear. Using histology in combination with immunohistochemistry and in situ hybridization in a retrospective review of a guinea pig confirmation-of-virulence study, we demonstrate for the first time Ebola virus infection in hepatic oval cells, the endocardium and stroma of the atrioventricular valves and chordae tendinae, satellite cells of peripheral ganglia, neurofibroblasts and Schwann cells of peripheral nerves and ganglia, smooth muscle cells of the uterine myometrium and vaginal wall, acini of the parotid salivary glands, thyroid follicular cells, adrenal medullary cells, pancreatic islet cells, endometrial glandular and surface epithelium, and the epithelium of the vagina, penis and, prepuce. These findings indicate that standard animal models for Ebola virus disease are not as well-described as previously thought and may serve as a stepping stone for future identification of potential sites of virus persistence.
format	article
author	Timothy K. Cooper Louis Huzella Joshua C. Johnson Oscar Rojas Sri Yellayi Mei G. Sun Sina Bavari Amanda Bonilla Randy Hart Peter B. Jahrling Jens H. Kuhn Xiankun Zeng
author_facet	Timothy K. Cooper Louis Huzella Joshua C. Johnson Oscar Rojas Sri Yellayi Mei G. Sun Sina Bavari Amanda Bonilla Randy Hart Peter B. Jahrling Jens H. Kuhn Xiankun Zeng
author_sort	Timothy K. Cooper
title	Histology, immunohistochemistry, and in situ hybridization reveal overlooked Ebola virus target tissues in the Ebola virus disease guinea pig model
title_short	Histology, immunohistochemistry, and in situ hybridization reveal overlooked Ebola virus target tissues in the Ebola virus disease guinea pig model
title_full	Histology, immunohistochemistry, and in situ hybridization reveal overlooked Ebola virus target tissues in the Ebola virus disease guinea pig model
title_fullStr	Histology, immunohistochemistry, and in situ hybridization reveal overlooked Ebola virus target tissues in the Ebola virus disease guinea pig model
title_full_unstemmed	Histology, immunohistochemistry, and in situ hybridization reveal overlooked Ebola virus target tissues in the Ebola virus disease guinea pig model
title_sort	histology, immunohistochemistry, and in situ hybridization reveal overlooked ebola virus target tissues in the ebola virus disease guinea pig model
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/948a329586ed44359c28050be99073b3
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Histology, immunohistochemistry, and in situ hybridization reveal overlooked Ebola virus target tissues in the Ebola virus disease guinea pig model

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