Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia

Abstract. Restykania, Suratman, Pitoyo A, Suranto. 2019. Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia. Biodiversitas 20: 3024-3032. The genetic diversity among madeira vine accessions from southeastern part of...

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Autores principales:	RESTYKANIA, suratman, ARI PITOYO, SURANTO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	anredera cordifolia, isozyme, madeira vine, morphology, southeastern part of central java Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/948a4f5306954976a8bb68af77cd5d36
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spelling	oai:doaj.org-article:948a4f5306954976a8bb68af77cd5d362021-11-21T21:54:57ZMorphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia1412-033X2085-472210.13057/biodiv/d201035https://doaj.org/article/948a4f5306954976a8bb68af77cd5d362019-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4387https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Restykania, Suratman, Pitoyo A, Suranto. 2019. Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia. Biodiversitas 20: 3024-3032. The genetic diversity among madeira vine accessions from southeastern part of Central Java has not been investigated in detail. Information on genetic diversity and relationship among accessions of this plant will provide important input into determining resourceful management strategies and guiding the improvement of plants through plant breeding programs. The objective of this study was to evaluate variation of morphology and isozyme among madeira vine (Anredera cordifolia (Ten.) Steenis) accessions from southeastern part of Central Java. A total of 13 madeira vine accessions were used for morphological observations and isozyme analysis. Morphological diversity was characterized based on measurements and observations of vegetative structures such as stems, leaves, and aerial tubers. Identification of biochemical markers was done using peroxidase and esterase isozyme systems. A relationship dendrogram among accessions was constructed based on the genetic similarity matrix by applying a cluster analysis. The analysis of quantitative and qualitative morphological characters revealed that the majority of the tested traits showed variation, indicating that there was variability among the tested madeira vine accessions. Polymorphism was observed using isozyme of peroxidase (i.e. two banding patterns) and esterase (i.e. five banding patterns). Based on the dendrogram at a level of 63 % similarity, it showed distinct separation of 13 madeira vine accessions into two major clusters. Cluster I consisted of nine accessions and the closest relationship was shown between KLT 3 and KLT 1 accessions that had 94 % of similarity coefficient. The remaining four accessions were clustered separately as Cluster II with similarity coefficient of 73 %. Therefore, genetic characterization based on morphological and isozyme markers obtained in this study is valuable to understand genetic variability and relationship among madeira vine accessions in southeastern part of Central Java.RESTYKANIAsuratmanARI PITOYOSURANTOMBI & UNS Soloarticleanredera cordifolia, isozyme, madeira vine, morphology, southeastern part of central javaBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	anredera cordifolia, isozyme, madeira vine, morphology, southeastern part of central java Biology (General) QH301-705.5
spellingShingle	anredera cordifolia, isozyme, madeira vine, morphology, southeastern part of central java Biology (General) QH301-705.5 RESTYKANIA suratman ARI PITOYO SURANTO Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia
description	Abstract. Restykania, Suratman, Pitoyo A, Suranto. 2019. Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia. Biodiversitas 20: 3024-3032. The genetic diversity among madeira vine accessions from southeastern part of Central Java has not been investigated in detail. Information on genetic diversity and relationship among accessions of this plant will provide important input into determining resourceful management strategies and guiding the improvement of plants through plant breeding programs. The objective of this study was to evaluate variation of morphology and isozyme among madeira vine (Anredera cordifolia (Ten.) Steenis) accessions from southeastern part of Central Java. A total of 13 madeira vine accessions were used for morphological observations and isozyme analysis. Morphological diversity was characterized based on measurements and observations of vegetative structures such as stems, leaves, and aerial tubers. Identification of biochemical markers was done using peroxidase and esterase isozyme systems. A relationship dendrogram among accessions was constructed based on the genetic similarity matrix by applying a cluster analysis. The analysis of quantitative and qualitative morphological characters revealed that the majority of the tested traits showed variation, indicating that there was variability among the tested madeira vine accessions. Polymorphism was observed using isozyme of peroxidase (i.e. two banding patterns) and esterase (i.e. five banding patterns). Based on the dendrogram at a level of 63 % similarity, it showed distinct separation of 13 madeira vine accessions into two major clusters. Cluster I consisted of nine accessions and the closest relationship was shown between KLT 3 and KLT 1 accessions that had 94 % of similarity coefficient. The remaining four accessions were clustered separately as Cluster II with similarity coefficient of 73 %. Therefore, genetic characterization based on morphological and isozyme markers obtained in this study is valuable to understand genetic variability and relationship among madeira vine accessions in southeastern part of Central Java.
format	article
author	RESTYKANIA suratman ARI PITOYO SURANTO
author_facet	RESTYKANIA suratman ARI PITOYO SURANTO
author_sort	RESTYKANIA
title	Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia
title_short	Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia
title_full	Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia
title_fullStr	Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia
title_full_unstemmed	Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia
title_sort	morphology and isozyme variation among madeira vine (anredera cordifolia) accessions from southeastern part of central java, indonesia
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/948a4f5306954976a8bb68af77cd5d36
work_keys_str_mv	AT restykania morphologyandisozymevariationamongmadeiravineanrederacordifoliaaccessionsfromsoutheasternpartofcentraljavaindonesia AT suratman morphologyandisozymevariationamongmadeiravineanrederacordifoliaaccessionsfromsoutheasternpartofcentraljavaindonesia AT aripitoyo morphologyandisozymevariationamongmadeiravineanrederacordifoliaaccessionsfromsoutheasternpartofcentraljavaindonesia AT suranto morphologyandisozymevariationamongmadeiravineanrederacordifoliaaccessionsfromsoutheasternpartofcentraljavaindonesia
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Morphology and isozyme variation among madeira vine (Anredera cordifolia) accessions from southeastern part of Central Java, Indonesia

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