Identification of miRNA-Target Gene Pairs Responsive to <i>Fusarium</i> Wilt of Cucumber via an Integrated Analysis of miRNA and Transcriptome Profiles
<i>Fusarium</i> wilt (<i>FW</i>) of cucumber (<i>Cucumis sativus</i> L.) caused by <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> (Foc) is a destructive soil-borne disease that severely decreases cucumber yield and quality worldwide. M...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/948aad17fb994374bb2ca81c0283fdff |
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| Sumario: | <i>Fusarium</i> wilt (<i>FW</i>) of cucumber (<i>Cucumis sativus</i> L.) caused by <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> (Foc) is a destructive soil-borne disease that severely decreases cucumber yield and quality worldwide. MicroRNAs (miRNAs) are small non-coding RNAs (sRNAs) that are important for regulating host immunity because they affect target gene expression. However, the specific miRNAs and the miRNA/target gene crosstalk involved in cucumber resistance to <i>FW</i> remain unknown. In this study, we compared sRNA-seq and RNA-seq data for cucumber cultivar ‘Rijiecheng’, which is resistant to <i>FW</i>. The integrated analysis identified <i>FW</i>-responsive miRNAs and their target genes. On the basis of verified expression levels, we detected two highly expressed miRNAs with down-regulated expression in response to Foc. Moreover, an analysis of 21 target genes in cucumber inoculated with Foc indicated that <i>JRL3</i> (<i>Csa2G362470</i>), which is targeted by <i>miR319a</i>, and <i>BEE1</i> (<i>Csa1G024150</i>), <i>DAHP1</i> (<i>Csa2G369040</i>), and <i>PERK2</i> (<i>Csa4G642480</i>), which are targeted by <i>miR6300</i>, are expressed at high levels, but their expression is further up-regulated after Foc inoculation. These results imply that <i>miR319a</i>-<i>JRL3</i>, <i>miR6300</i>-<i>BEE1</i>, <i>miR6300</i>-<i>DAHP1</i> and <i>miR6300</i>-<i>PERK2</i> regulate cucumber defenses against <i>FW</i>, and provide the gene resources that may be useful for breeding programs focused on developing new cucumber varieties with enhanced resistance to <i>FW</i>. |
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