Antitrypanosomal Activity of Anthriscus Nemorosa Essential Oils and Combinations of Their Main Constituents

This study aimed to investigate the susceptibility of Trypanosoma brucei to the Anthriscus nemorosa essential oils (EOs), isolated compounds from these oils, and artificial mixtures of the isolated compounds in their conventional and nanoencapsulated forms. The...

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Autores principales:	Cecilia Baldassarri, Giulia Falappa, Eugenia Mazzara, Laura Acquaticci, Elena Ossoli, Diego Romano Perinelli, Giulia Bonacucina, Stefano Dall’Acqua, Loredana Cappellacci, Filippo Maggi, Farahnaz Ranjbarian, Anders Hofer, Riccardo Petrelli
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>A. nemorosa</i> <i>Trypanosoma brucei</i> farnesene nanoemulsions artificial mixtures Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/948d155ad812401b81bf7e1071b0c755
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spelling	oai:doaj.org-article:948d155ad812401b81bf7e1071b0c7552021-11-25T16:25:00ZAntitrypanosomal Activity of <i>Anthriscus</i> Nemorosa Essential Oils and Combinations of Their Main Constituents10.3390/antibiotics101114132079-6382https://doaj.org/article/948d155ad812401b81bf7e1071b0c7552021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1413https://doaj.org/toc/2079-6382This study aimed to investigate the susceptibility of <i>Trypanosoma brucei</i> to the <i>Anthriscus nemorosa</i> essential oils (EOs), isolated compounds from these oils, and artificial mixtures of the isolated compounds in their conventional and nanoencapsulated forms. The chemical composition of the essential oils from the aerial parts and roots of <i>Anthriscus nemorosa</i>, obtained from a wild population growing in central Italy, were analyzed by gas chromatography/mass spectrometry (GC/MS). In both cases, the predominant class of compounds was monoterpene hydrocarbons, which were more abundant in the EOs from the roots (81.5%) than the aerial parts (74.0%). The overall results of this work have shed light on the biological properties of <i>A. nemorosa</i> EO from aerial parts (EC<sub>50</sub> = 1.17 μg/mL), farnesene (EC<sub>50</sub> = 0.84 μg/mL), and artificial mixtures (Mix 3–5, EC<sub>50</sub> in the range of 1.27 to 1.58 μg/mL) as relevant sources of antiprotozoal substances. Furthermore, the pool measurements of ADP (adenosine diphosphate) and NTPs (nucleoside triphosphates) in the cultivated bloodstream form of trypanosomes exposed to different concentrations of EOs showed a disturbed energy metabolism, as indicated by increased pools of ADP in comparison to ATP (adenosine triphosphate) and other NTPs. Ultimately, this study highlights the significant efficacy of <i>A. nemorosa</i> EO to develop long-lasting and effective antiprotozoal formulations, including nanoemulsions.Cecilia BaldassarriGiulia FalappaEugenia MazzaraLaura AcquaticciElena OssoliDiego Romano PerinelliGiulia BonacucinaStefano Dall’AcquaLoredana CappellacciFilippo MaggiFarahnaz RanjbarianAnders HoferRiccardo PetrelliMDPI AGarticle<i>A. nemorosa</i><i>Trypanosoma brucei</i>farnesenenanoemulsionsartificial mixturesTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1413, p 1413 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>A. nemorosa</i> <i>Trypanosoma brucei</i> farnesene nanoemulsions artificial mixtures Therapeutics. Pharmacology RM1-950
spellingShingle	<i>A. nemorosa</i> <i>Trypanosoma brucei</i> farnesene nanoemulsions artificial mixtures Therapeutics. Pharmacology RM1-950 Cecilia Baldassarri Giulia Falappa Eugenia Mazzara Laura Acquaticci Elena Ossoli Diego Romano Perinelli Giulia Bonacucina Stefano Dall’Acqua Loredana Cappellacci Filippo Maggi Farahnaz Ranjbarian Anders Hofer Riccardo Petrelli Antitrypanosomal Activity of <i>Anthriscus</i> Nemorosa Essential Oils and Combinations of Their Main Constituents
description	This study aimed to investigate the susceptibility of <i>Trypanosoma brucei</i> to the <i>Anthriscus nemorosa</i> essential oils (EOs), isolated compounds from these oils, and artificial mixtures of the isolated compounds in their conventional and nanoencapsulated forms. The chemical composition of the essential oils from the aerial parts and roots of <i>Anthriscus nemorosa</i>, obtained from a wild population growing in central Italy, were analyzed by gas chromatography/mass spectrometry (GC/MS). In both cases, the predominant class of compounds was monoterpene hydrocarbons, which were more abundant in the EOs from the roots (81.5%) than the aerial parts (74.0%). The overall results of this work have shed light on the biological properties of <i>A. nemorosa</i> EO from aerial parts (EC<sub>50</sub> = 1.17 μg/mL), farnesene (EC<sub>50</sub> = 0.84 μg/mL), and artificial mixtures (Mix 3–5, EC<sub>50</sub> in the range of 1.27 to 1.58 μg/mL) as relevant sources of antiprotozoal substances. Furthermore, the pool measurements of ADP (adenosine diphosphate) and NTPs (nucleoside triphosphates) in the cultivated bloodstream form of trypanosomes exposed to different concentrations of EOs showed a disturbed energy metabolism, as indicated by increased pools of ADP in comparison to ATP (adenosine triphosphate) and other NTPs. Ultimately, this study highlights the significant efficacy of <i>A. nemorosa</i> EO to develop long-lasting and effective antiprotozoal formulations, including nanoemulsions.
format	article
author	Cecilia Baldassarri Giulia Falappa Eugenia Mazzara Laura Acquaticci Elena Ossoli Diego Romano Perinelli Giulia Bonacucina Stefano Dall’Acqua Loredana Cappellacci Filippo Maggi Farahnaz Ranjbarian Anders Hofer Riccardo Petrelli
author_facet	Cecilia Baldassarri Giulia Falappa Eugenia Mazzara Laura Acquaticci Elena Ossoli Diego Romano Perinelli Giulia Bonacucina Stefano Dall’Acqua Loredana Cappellacci Filippo Maggi Farahnaz Ranjbarian Anders Hofer Riccardo Petrelli
author_sort	Cecilia Baldassarri
title	Antitrypanosomal Activity of <i>Anthriscus</i> Nemorosa Essential Oils and Combinations of Their Main Constituents
title_short	Antitrypanosomal Activity of <i>Anthriscus</i> Nemorosa Essential Oils and Combinations of Their Main Constituents
title_full	Antitrypanosomal Activity of <i>Anthriscus</i> Nemorosa Essential Oils and Combinations of Their Main Constituents
title_fullStr	Antitrypanosomal Activity of <i>Anthriscus</i> Nemorosa Essential Oils and Combinations of Their Main Constituents
title_full_unstemmed	Antitrypanosomal Activity of <i>Anthriscus</i> Nemorosa Essential Oils and Combinations of Their Main Constituents
title_sort	antitrypanosomal activity of <i>anthriscus</i> nemorosa essential oils and combinations of their main constituents
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/948d155ad812401b81bf7e1071b0c755
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Antitrypanosomal Activity of <i>Anthriscus</i> Nemorosa Essential Oils and Combinations of Their Main Constituents

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