Reduction in breast cancer susceptibility due to XbaI gene polymorphism of alpha estrogen receptor gene in Jordanians

Manar Fayiz Atoum, Foad Alzoughool Department of Medical Laboratory Sciences, Faculty of Allied Health, Hashemite University, Zarqa, Jordan Abstract: Breast cancer is a global health concern among women worldwide. Estrogen receptor alpha (ERα) mediates diverse polymorphic effects...

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Autores principales:	Atoum MF, Alzoughool F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	breast cancer gene polymorphism XbaI Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/949e0f2a42b84c91977aa3413e24c364
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spelling	oai:doaj.org-article:949e0f2a42b84c91977aa3413e24c3642021-12-02T11:15:48ZReduction in breast cancer susceptibility due to XbaI gene polymorphism of alpha estrogen receptor gene in Jordanians1179-1314https://doaj.org/article/949e0f2a42b84c91977aa3413e24c3642017-01-01T00:00:00Zhttps://www.dovepress.com/reduction-in-breast-cancer-susceptibility-due-to-xbai-gene-polymorphis-peer-reviewed-article-BCTThttps://doaj.org/toc/1179-1314Manar Fayiz Atoum, Foad Alzoughool Department of Medical Laboratory Sciences, Faculty of Allied Health, Hashemite University, Zarqa, Jordan Abstract: Breast cancer is a global health concern among women worldwide. Estrogen receptor alpha (ERα) mediates diverse polymorphic effects in breast tissues that may relate to breast cancer susceptibility. The aim of this study was to evaluate the effect of −397 PvuII (T/C) and −351 XbaI (A/G) restriction fragment length polymorphism within intron 1 of ERα, and its effect on breast cancer susceptibility. A total of 156 women who were histopathologically diagnosed with breast cancer and 142 healthy Jordanian women were enrolled in this case–control study. Genomic DNA was extracted from whole peripheral blood, and the desired fragment was amplified using polymerase chain reaction followed by restriction digestion with PvuII and XbaI restriction enzymes. The results showed no significant association between PvuII polymorphism and breast cancer risk. However, a significant association was found between XbaI polymorphism and reduction in breast cancer risk within the “x” allele of heterozygotes (odds ratio [OR] 0.199, 95% confidence interval [CI] 0.09–0.044) and heterozygotes (OR 0.208, 95% CI 0.09–0.047). The combined analysis of PvuII and XbaI polymorphisms revealed a synergistic effect of Pp/Xx and pp/xx genotypes and a significant reduction in breast cancer risk with these genotypes. The results also showed no statistical differences among PvuII or XbaI polymorphisms based on stage, ER, progesterone receptor and expression of hormone receptor such as human epidermal growth factor receptor 2. This case–control study showed that XbaI polymorphism of alpha estrogen gene modified and reduced breast cancer susceptibility among Jordanians. Keywords: breast cancer, gene polymorphism, XbaIAtoum MFAlzoughool FDove Medical Pressarticlebreast cancergene polymorphismXbaINeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBreast Cancer: Targets and Therapy, Vol Volume 9, Pp 45-49 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer gene polymorphism XbaI Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	breast cancer gene polymorphism XbaI Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Atoum MF Alzoughool F Reduction in breast cancer susceptibility due to XbaI gene polymorphism of alpha estrogen receptor gene in Jordanians
description	Manar Fayiz Atoum, Foad Alzoughool Department of Medical Laboratory Sciences, Faculty of Allied Health, Hashemite University, Zarqa, Jordan Abstract: Breast cancer is a global health concern among women worldwide. Estrogen receptor alpha (ERα) mediates diverse polymorphic effects in breast tissues that may relate to breast cancer susceptibility. The aim of this study was to evaluate the effect of −397 PvuII (T/C) and −351 XbaI (A/G) restriction fragment length polymorphism within intron 1 of ERα, and its effect on breast cancer susceptibility. A total of 156 women who were histopathologically diagnosed with breast cancer and 142 healthy Jordanian women were enrolled in this case–control study. Genomic DNA was extracted from whole peripheral blood, and the desired fragment was amplified using polymerase chain reaction followed by restriction digestion with PvuII and XbaI restriction enzymes. The results showed no significant association between PvuII polymorphism and breast cancer risk. However, a significant association was found between XbaI polymorphism and reduction in breast cancer risk within the “x” allele of heterozygotes (odds ratio [OR] 0.199, 95% confidence interval [CI] 0.09–0.044) and heterozygotes (OR 0.208, 95% CI 0.09–0.047). The combined analysis of PvuII and XbaI polymorphisms revealed a synergistic effect of Pp/Xx and pp/xx genotypes and a significant reduction in breast cancer risk with these genotypes. The results also showed no statistical differences among PvuII or XbaI polymorphisms based on stage, ER, progesterone receptor and expression of hormone receptor such as human epidermal growth factor receptor 2. This case–control study showed that XbaI polymorphism of alpha estrogen gene modified and reduced breast cancer susceptibility among Jordanians. Keywords: breast cancer, gene polymorphism, XbaI
format	article
author	Atoum MF Alzoughool F
author_facet	Atoum MF Alzoughool F
author_sort	Atoum MF
title	Reduction in breast cancer susceptibility due to XbaI gene polymorphism of alpha estrogen receptor gene in Jordanians
title_short	Reduction in breast cancer susceptibility due to XbaI gene polymorphism of alpha estrogen receptor gene in Jordanians
title_full	Reduction in breast cancer susceptibility due to XbaI gene polymorphism of alpha estrogen receptor gene in Jordanians
title_fullStr	Reduction in breast cancer susceptibility due to XbaI gene polymorphism of alpha estrogen receptor gene in Jordanians
title_full_unstemmed	Reduction in breast cancer susceptibility due to XbaI gene polymorphism of alpha estrogen receptor gene in Jordanians
title_sort	reduction in breast cancer susceptibility due to xbai gene polymorphism of alpha estrogen receptor gene in jordanians
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/949e0f2a42b84c91977aa3413e24c364
work_keys_str_mv	AT atoummf reductioninbreastcancersusceptibilityduetoxbaigenepolymorphismofalphaestrogenreceptorgeneinjordanians AT alzoughoolf reductioninbreastcancersusceptibilityduetoxbaigenepolymorphismofalphaestrogenreceptorgeneinjordanians
_version_	1718396054083207168

Reduction in breast cancer susceptibility due to XbaI gene polymorphism of alpha estrogen receptor gene in Jordanians

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