Chronic cadmium exposure decreases the dependency of MCF7 breast cancer cells on ERα

Abstract Cadmium is an environmental contaminant that can activate estrogen receptor alpha (ERα) and contribute to the development and progression of breast cancer. Our lab previously demonstrated that chronic cadmium exposure alters the expression of several ERα-responsive genes and increases the m...

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Autores principales:	Mathew Bloomfield, Maggie C. Louie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/94a37b9660594765808bea94d3ce032c
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spelling	oai:doaj.org-article:94a37b9660594765808bea94d3ce032c2021-12-02T15:08:10ZChronic cadmium exposure decreases the dependency of MCF7 breast cancer cells on ERα10.1038/s41598-019-46912-32045-2322https://doaj.org/article/94a37b9660594765808bea94d3ce032c2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46912-3https://doaj.org/toc/2045-2322Abstract Cadmium is an environmental contaminant that can activate estrogen receptor alpha (ERα) and contribute to the development and progression of breast cancer. Our lab previously demonstrated that chronic cadmium exposure alters the expression of several ERα-responsive genes and increases the malignancy of breast cancer cells. Although these studies support cadmium’s function as a hormone disrupter, the role of ERα in cadmium-induced breast cancer progression remains unclear. To address this, we modulated the expression of ERα and found that while the loss of ERα significantly impaired cancer cell growth, migration, invasion and anchorage-independent growth in both MCF7 and MCF7-Cd cells, cadmium-exposed cells retained a significant advantage in cell growth, migration, and invasion, and partially circumvented the loss of ERα. ERα knockout in MCF7 and MCF7-Cd cells significantly reduced the expression of classical ERα-regulated genes, while non-classical ERα-regulated genes were less impacted by the loss of ERα in MCF7-Cd cells. This is the first study to show that chronic cadmium exposure, even at low levels, can increase the malignancy of breast cancer cells by decreasing their dependency on ERα and increasing the adaptability of the cancer cells.Mathew BloomfieldMaggie C. LouieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mathew Bloomfield Maggie C. Louie Chronic cadmium exposure decreases the dependency of MCF7 breast cancer cells on ERα
description	Abstract Cadmium is an environmental contaminant that can activate estrogen receptor alpha (ERα) and contribute to the development and progression of breast cancer. Our lab previously demonstrated that chronic cadmium exposure alters the expression of several ERα-responsive genes and increases the malignancy of breast cancer cells. Although these studies support cadmium’s function as a hormone disrupter, the role of ERα in cadmium-induced breast cancer progression remains unclear. To address this, we modulated the expression of ERα and found that while the loss of ERα significantly impaired cancer cell growth, migration, invasion and anchorage-independent growth in both MCF7 and MCF7-Cd cells, cadmium-exposed cells retained a significant advantage in cell growth, migration, and invasion, and partially circumvented the loss of ERα. ERα knockout in MCF7 and MCF7-Cd cells significantly reduced the expression of classical ERα-regulated genes, while non-classical ERα-regulated genes were less impacted by the loss of ERα in MCF7-Cd cells. This is the first study to show that chronic cadmium exposure, even at low levels, can increase the malignancy of breast cancer cells by decreasing their dependency on ERα and increasing the adaptability of the cancer cells.
format	article
author	Mathew Bloomfield Maggie C. Louie
author_facet	Mathew Bloomfield Maggie C. Louie
author_sort	Mathew Bloomfield
title	Chronic cadmium exposure decreases the dependency of MCF7 breast cancer cells on ERα
title_short	Chronic cadmium exposure decreases the dependency of MCF7 breast cancer cells on ERα
title_full	Chronic cadmium exposure decreases the dependency of MCF7 breast cancer cells on ERα
title_fullStr	Chronic cadmium exposure decreases the dependency of MCF7 breast cancer cells on ERα
title_full_unstemmed	Chronic cadmium exposure decreases the dependency of MCF7 breast cancer cells on ERα
title_sort	chronic cadmium exposure decreases the dependency of mcf7 breast cancer cells on erα
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/94a37b9660594765808bea94d3ce032c
work_keys_str_mv	AT mathewbloomfield chroniccadmiumexposuredecreasesthedependencyofmcf7breastcancercellsonera AT maggieclouie chroniccadmiumexposuredecreasesthedependencyofmcf7breastcancercellsonera
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Chronic cadmium exposure decreases the dependency of MCF7 breast cancer cells on ERα

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