Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia

Abstract. Dinoto A, Rosyidah A, Susilo ARPS, Julistiono H. 2020. Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia. Biodiversitas 21: 3391-3397. Presence of culturable lactic acid bacteria (LAB) in frui...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Achmad Dinoto, A’liyatur Rosyidah, Anggi Ria Puspitasari Susilo, Heddy Julistiono
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	antimicrobial activity, escherichia coli, lactic acid bacteria, mycobacterium smegmatis, staphylococcus aureus, wild fruits Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/94a4e21a01d942deaeb5b3b0353379bb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:94a4e21a01d942deaeb5b3b0353379bb
record_format	dspace
spelling	oai:doaj.org-article:94a4e21a01d942deaeb5b3b0353379bb2021-11-22T00:40:51ZIsolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia1412-033X2085-472210.13057/biodiv/d210764https://doaj.org/article/94a4e21a01d942deaeb5b3b0353379bb2020-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5822https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Dinoto A, Rosyidah A, Susilo ARPS, Julistiono H. 2020. Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia. Biodiversitas 21: 3391-3397. Presence of culturable lactic acid bacteria (LAB) in fruits of wild plants and their antimicrobial activities has not been widely reported. The purposes of this study were to isolate LAB from the fruits of wild plants found in the Tambrauw forest area, West Papua Indonesia, and to evaluate their antimicrobial activities. Isolation of LAB from fruit was conducted using MRS medium supplemented with 1% CaCO3. Isolates of LAB were identified based on 16S rRNA gene using BLAST analysis. Antimicrobial assays were carried out by determining the minimum inhibitory concentration (MIC) based on thiazolyl blue tetrazolium blue (MTT) using indicator microorganisms Escherichia coli, Staphylococcus aureus, and Mycobacterium smegmatis. The results showed that total of fourteen isolates of LAB with different characteristics was successfully isolated from 8 of 14 collected wild plants. Based on 16S rRNA sequences, isolates had closest relationships with Lactococcus lactis, Lactococcus garvieae, Weissella confusa, Weissella oryzae, and Enterococcus faecalis with the similarity of 99%. All 16S rRNA nucleotides of these strains have been deposited in the GenBank. Assays for antimicrobial activities were demonstrated by the highest inhibition of supernatant of Lac. lactis HM 1.1 from fruit plant Donax canniformis and W. confusa H14.2 from fruit plant Capparis sp. against E. coli, S. aureus, and M. smegmatis even though the MIC values of those strains were lower than that of bacterial strain from the commercial probiotic product. This study showed that wild fruit from Tambrauw forest harbor beneficial lactic acid bacteria that could be important for health of animals and humans as well. In addition, this study provided basic information on indigenous LAB for promoting further development of medicinal antibacterial compounds.Achmad DinotoA’liyatur RosyidahAnggi Ria Puspitasari SusiloHeddy JulistionoMBI & UNS Soloarticleantimicrobial activity, escherichia coli, lactic acid bacteria, mycobacterium smegmatis, staphylococcus aureus, wild fruitsBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial activity, escherichia coli, lactic acid bacteria, mycobacterium smegmatis, staphylococcus aureus, wild fruits Biology (General) QH301-705.5
spellingShingle	antimicrobial activity, escherichia coli, lactic acid bacteria, mycobacterium smegmatis, staphylococcus aureus, wild fruits Biology (General) QH301-705.5 Achmad Dinoto A’liyatur Rosyidah Anggi Ria Puspitasari Susilo Heddy Julistiono Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia
description	Abstract. Dinoto A, Rosyidah A, Susilo ARPS, Julistiono H. 2020. Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia. Biodiversitas 21: 3391-3397. Presence of culturable lactic acid bacteria (LAB) in fruits of wild plants and their antimicrobial activities has not been widely reported. The purposes of this study were to isolate LAB from the fruits of wild plants found in the Tambrauw forest area, West Papua Indonesia, and to evaluate their antimicrobial activities. Isolation of LAB from fruit was conducted using MRS medium supplemented with 1% CaCO3. Isolates of LAB were identified based on 16S rRNA gene using BLAST analysis. Antimicrobial assays were carried out by determining the minimum inhibitory concentration (MIC) based on thiazolyl blue tetrazolium blue (MTT) using indicator microorganisms Escherichia coli, Staphylococcus aureus, and Mycobacterium smegmatis. The results showed that total of fourteen isolates of LAB with different characteristics was successfully isolated from 8 of 14 collected wild plants. Based on 16S rRNA sequences, isolates had closest relationships with Lactococcus lactis, Lactococcus garvieae, Weissella confusa, Weissella oryzae, and Enterococcus faecalis with the similarity of 99%. All 16S rRNA nucleotides of these strains have been deposited in the GenBank. Assays for antimicrobial activities were demonstrated by the highest inhibition of supernatant of Lac. lactis HM 1.1 from fruit plant Donax canniformis and W. confusa H14.2 from fruit plant Capparis sp. against E. coli, S. aureus, and M. smegmatis even though the MIC values of those strains were lower than that of bacterial strain from the commercial probiotic product. This study showed that wild fruit from Tambrauw forest harbor beneficial lactic acid bacteria that could be important for health of animals and humans as well. In addition, this study provided basic information on indigenous LAB for promoting further development of medicinal antibacterial compounds.
format	article
author	Achmad Dinoto A’liyatur Rosyidah Anggi Ria Puspitasari Susilo Heddy Julistiono
author_facet	Achmad Dinoto A’liyatur Rosyidah Anggi Ria Puspitasari Susilo Heddy Julistiono
author_sort	Achmad Dinoto
title	Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia
title_short	Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia
title_full	Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia
title_fullStr	Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia
title_full_unstemmed	Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia
title_sort	isolation, identification and antimicrobial activities of lactic acid bacteria from fruits of wild plants in tambrauw forest, west papua, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/94a4e21a01d942deaeb5b3b0353379bb
work_keys_str_mv	AT achmaddinoto isolationidentificationandantimicrobialactivitiesoflacticacidbacteriafromfruitsofwildplantsintambrauwforestwestpapuaindonesia AT aliyaturrosyidah isolationidentificationandantimicrobialactivitiesoflacticacidbacteriafromfruitsofwildplantsintambrauwforestwestpapuaindonesia AT anggiriapuspitasarisusilo isolationidentificationandantimicrobialactivitiesoflacticacidbacteriafromfruitsofwildplantsintambrauwforestwestpapuaindonesia AT heddyjulistiono isolationidentificationandantimicrobialactivitiesoflacticacidbacteriafromfruitsofwildplantsintambrauwforestwestpapuaindonesia
_version_	1718418538515922944

Isolation, identification and antimicrobial activities of Lactic Acid Bacteria from fruits of wild plants in Tambrauw Forest, West Papua, Indonesia

Ejemplares similares