Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy

Chen Shao, Kun Shang, Huaibao Xu, Yu Zhang, Zhichao Pei, Yuxin Pei Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi 712100, China Background: Photodynamic therapy is a safe, noninvasi...

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Autores principales:	Shao C, Shang K, Xu H, Zhang Y, Pei Z, Pei Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	hypericin photodynamic therapy glyconanoparticles targeted drug delivery Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/94b0d393fb044aac8bc03ee59559d138
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spelling	oai:doaj.org-article:94b0d393fb044aac8bc03ee59559d1382021-12-02T05:10:09ZFacile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy1178-2013https://doaj.org/article/94b0d393fb044aac8bc03ee59559d1382018-07-01T00:00:00Zhttps://www.dovepress.com/facile-fabrication-of-hypericin-entrapped-glyconanoparticles-for-targe-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chen Shao, Kun Shang, Huaibao Xu, Yu Zhang, Zhichao Pei, Yuxin Pei Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi 712100, China Background: Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component. Hypericin (Hy) is a promising PS; however, its clinical application is significantly limited by its poor hydrophilicity. Materials and methods: To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition. The amount of Hy in the Hy-entrapped PDA–MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry. Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups. Results: Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 µmol g-1 PHMs. The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells. Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs. The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells. Conclusion: The strategy developed in this work offers great potential for delivery of a variety of hydrophobic PSs. Keywords: hypericin, photodynamic therapy, glyconanoparticles, targeted drug deliveryShao CShang KXu HZhang YPei ZPei YDove Medical Pressarticlehypericinphotodynamic therapyglyconanoparticlestargeted drug deliveryMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4319-4331 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	hypericin photodynamic therapy glyconanoparticles targeted drug delivery Medicine (General) R5-920
spellingShingle	hypericin photodynamic therapy glyconanoparticles targeted drug delivery Medicine (General) R5-920 Shao C Shang K Xu H Zhang Y Pei Z Pei Y Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy
description	Chen Shao, Kun Shang, Huaibao Xu, Yu Zhang, Zhichao Pei, Yuxin Pei Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi 712100, China Background: Photodynamic therapy is a safe, noninvasive modality for cancer therapy, in which the photosensitizer (PS) is a crucial component. Hypericin (Hy) is a promising PS; however, its clinical application is significantly limited by its poor hydrophilicity. Materials and methods: To overcome the clinical application limitation of Hy, a novel strategy is developed here by entrapping Hy into polydopamine (PDA) film formed on the surface of magnetic iron oxide nanoparticles (MNPs) through the self-polymerization of dopamine under alkaline condition. The amount of Hy in the Hy-entrapped PDA–MNP composite nanoparticles (denoted as PHMs) was measured by spectrophotometry. Furthermore, lactose, as the targeting ligand to asialoglycoprotein receptors, was conjugated to the surface of the PHMs by taking advantage of the spontaneous reaction of PDA with amino groups. Results: Spectrophotometry analysis revealed that the amount of Hy in the PHMs was 72 µmol g-1 PHMs. The fabricated Hy-entrapped glyconanoparticle (Lac-PHM) exhibited excellent water dispersibility, stability, and selectivity for asialoglycoprotein receptors overexpressing HepG2 cells. Atomic absorption spectroscopy analysis showed that the amount of the Lac-PHMs taken in HepG2 cells was 2.1-fold higher than that of the triethylene glycol-modified PHMs. The results of intracellular reactive oxygen species generation detection, cytotoxicity study, and apoptosis detection indicated that the Lac-PHMs had a satisfying photodynamic effect to HepG2 cells. Conclusion: The strategy developed in this work offers great potential for delivery of a variety of hydrophobic PSs. Keywords: hypericin, photodynamic therapy, glyconanoparticles, targeted drug delivery
format	article
author	Shao C Shang K Xu H Zhang Y Pei Z Pei Y
author_facet	Shao C Shang K Xu H Zhang Y Pei Z Pei Y
author_sort	Shao C
title	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy
title_short	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy
title_full	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy
title_fullStr	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy
title_full_unstemmed	Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy
title_sort	facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/94b0d393fb044aac8bc03ee59559d138
work_keys_str_mv	AT shaoc facilefabricationofhypericinentrappedglyconanoparticlesfortargetedphotodynamictherapy AT shangk facilefabricationofhypericinentrappedglyconanoparticlesfortargetedphotodynamictherapy AT xuh facilefabricationofhypericinentrappedglyconanoparticlesfortargetedphotodynamictherapy AT zhangy facilefabricationofhypericinentrappedglyconanoparticlesfortargetedphotodynamictherapy AT peiz facilefabricationofhypericinentrappedglyconanoparticlesfortargetedphotodynamictherapy AT peiy facilefabricationofhypericinentrappedglyconanoparticlesfortargetedphotodynamictherapy
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Facile fabrication of hypericin-entrapped glyconanoparticles for targeted photodynamic therapy

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