In-situ quantification of microscopic contributions of individual cells to macroscopic wood deformation with synchrotron computed tomography

Abstract Wood-based composites hold the promise of sustainable construction. Understanding the influence on wood cellular microstructure in the macroscopic mechanical behavior is key for engineering high-performance composites. In this work, we report a novel Individual Cell Tracking (ICT) approach...

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Autores principales:	Sergio J. Sanabria, Franziska Baensch, Michaela Zauner, Peter Niemz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/94b3e1937c0b48a3a696f28810794921
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spelling	oai:doaj.org-article:94b3e1937c0b48a3a696f288107949212021-12-02T15:11:50ZIn-situ quantification of microscopic contributions of individual cells to macroscopic wood deformation with synchrotron computed tomography10.1038/s41598-020-78028-42045-2322https://doaj.org/article/94b3e1937c0b48a3a696f288107949212020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78028-4https://doaj.org/toc/2045-2322Abstract Wood-based composites hold the promise of sustainable construction. Understanding the influence on wood cellular microstructure in the macroscopic mechanical behavior is key for engineering high-performance composites. In this work, we report a novel Individual Cell Tracking (ICT) approach for in-situ quantification of nanometer-scale deformations of individual wood cells during mechanical loading of macroscopic millimeter-scale wood samples. Softwood samples containing > 104 cells were subjected to controlled radial tensile and longitudinal compressive load in a synchrotron radiation micro-computed tomography (SRµCT) setup. Tracheid and wood ray cells were automatically segmented, and their geometric variations were tracked during load. Finally, interactions between microstructure deformations (lumen geometry, cell wall thickness), cellular arrangement (annual growth rings, anisotropy, wood ray presence) with the macroscopic deformation response were investigated. The results provide cellular insight into macroscopic relations, such as anisotropic Poisson effects, and allow direct observation of previously suspected wood ray reinforcing effects. The method is also appropriate for investigation of non-linear deformation effects, such as buckling and deformation recovery after failure, and gives insight into less studied aspects, such as changes in lumen diameter and cell wall thickness during uniaxial load. ICT provides an experimental tool for direct validation of hierarchical mechanical models on real biological composites.Sergio J. SanabriaFranziska BaenschMichaela ZaunerPeter NiemzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sergio J. Sanabria Franziska Baensch Michaela Zauner Peter Niemz In-situ quantification of microscopic contributions of individual cells to macroscopic wood deformation with synchrotron computed tomography
description	Abstract Wood-based composites hold the promise of sustainable construction. Understanding the influence on wood cellular microstructure in the macroscopic mechanical behavior is key for engineering high-performance composites. In this work, we report a novel Individual Cell Tracking (ICT) approach for in-situ quantification of nanometer-scale deformations of individual wood cells during mechanical loading of macroscopic millimeter-scale wood samples. Softwood samples containing > 104 cells were subjected to controlled radial tensile and longitudinal compressive load in a synchrotron radiation micro-computed tomography (SRµCT) setup. Tracheid and wood ray cells were automatically segmented, and their geometric variations were tracked during load. Finally, interactions between microstructure deformations (lumen geometry, cell wall thickness), cellular arrangement (annual growth rings, anisotropy, wood ray presence) with the macroscopic deformation response were investigated. The results provide cellular insight into macroscopic relations, such as anisotropic Poisson effects, and allow direct observation of previously suspected wood ray reinforcing effects. The method is also appropriate for investigation of non-linear deformation effects, such as buckling and deformation recovery after failure, and gives insight into less studied aspects, such as changes in lumen diameter and cell wall thickness during uniaxial load. ICT provides an experimental tool for direct validation of hierarchical mechanical models on real biological composites.
format	article
author	Sergio J. Sanabria Franziska Baensch Michaela Zauner Peter Niemz
author_facet	Sergio J. Sanabria Franziska Baensch Michaela Zauner Peter Niemz
author_sort	Sergio J. Sanabria
title	In-situ quantification of microscopic contributions of individual cells to macroscopic wood deformation with synchrotron computed tomography
title_short	In-situ quantification of microscopic contributions of individual cells to macroscopic wood deformation with synchrotron computed tomography
title_full	In-situ quantification of microscopic contributions of individual cells to macroscopic wood deformation with synchrotron computed tomography
title_fullStr	In-situ quantification of microscopic contributions of individual cells to macroscopic wood deformation with synchrotron computed tomography
title_full_unstemmed	In-situ quantification of microscopic contributions of individual cells to macroscopic wood deformation with synchrotron computed tomography
title_sort	in-situ quantification of microscopic contributions of individual cells to macroscopic wood deformation with synchrotron computed tomography
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/94b3e1937c0b48a3a696f28810794921
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In-situ quantification of microscopic contributions of individual cells to macroscopic wood deformation with synchrotron computed tomography

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