Identification of candidate genes from androgenic gland in Macrobrachium nipponense regulated by eyestalk ablation

Abstract The eyestalk of crustaceans, such as Macrobrachium nipponense, contains many neurosecretory hormones affecting the process of reproduction, molting, metabolism of glucose, and other functions. In this study, important metabolic pathways and candidate genes involved in male sexual developmen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shubo Jin, Yin Fu, Yuning Hu, Hongtuo Fu, Sufei Jiang, Yiwei Xiong, Hui Qiao, Wenyi Zhang, Yongsheng Gong, Yan Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/94b5c56ae8694c9186308e880760a42d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:94b5c56ae8694c9186308e880760a42d
record_format	dspace
spelling	oai:doaj.org-article:94b5c56ae8694c9186308e880760a42d2021-12-02T19:16:19ZIdentification of candidate genes from androgenic gland in Macrobrachium nipponense regulated by eyestalk ablation10.1038/s41598-021-99022-42045-2322https://doaj.org/article/94b5c56ae8694c9186308e880760a42d2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99022-4https://doaj.org/toc/2045-2322Abstract The eyestalk of crustaceans, such as Macrobrachium nipponense, contains many neurosecretory hormones affecting the process of reproduction, molting, metabolism of glucose, and other functions. In this study, important metabolic pathways and candidate genes involved in male sexual development were selected from M. nipponense. The methodology involved performing long-read and next generation transcriptome sequencing of genes from the androgenic gland after eyestalk ablation. qPCR analysis revealed that the mRNA expression of Mn-IAG was significantly increased after ablation of both the single-side (SS) and double-side (DS) eyestalk, compared with the control group (CG). The long-read transcriptome generated 49,840 non-redundant transcripts. A total of 1319, 2092 and 4351 differentially expressed genes (DEGs) were identified between CG versus SS, SS versus DS and CG versus DS, respectively. These data indicated that ablation of the double-sided eyestalk played stronger regulatory roles than the single-side ablation on male sexual development in M. nipponense. This was consistent with the qPCR analysis. Cell Cycle, Cellular Senescence, Oxidative Phosphorylation, Glycolysis/Gluconeogenesis and Steroid Hormone Biosynthesis were the primary enriched metabolic pathways in all three comparisons, and the important genes from these metabolic pathways were also selected. qPCR permitted secondary confirmation of ten DEGs identified through RNA-seq. RNAi-mediated silencing analyses of Hydroxysteroid dehydrogenase like 1 (HSDL1) revealed that HSDL1 has a positive regulatory effect on testes development. This study provides valuable insight into male sexual development in M. nipponense, including metabolic pathways and genes, paving the way for advanced studies on male sexual development in this species and in other crustaceans.Shubo JinYin FuYuning HuHongtuo FuSufei JiangYiwei XiongHui QiaoWenyi ZhangYongsheng GongYan WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shubo Jin Yin Fu Yuning Hu Hongtuo Fu Sufei Jiang Yiwei Xiong Hui Qiao Wenyi Zhang Yongsheng Gong Yan Wu Identification of candidate genes from androgenic gland in Macrobrachium nipponense regulated by eyestalk ablation
description	Abstract The eyestalk of crustaceans, such as Macrobrachium nipponense, contains many neurosecretory hormones affecting the process of reproduction, molting, metabolism of glucose, and other functions. In this study, important metabolic pathways and candidate genes involved in male sexual development were selected from M. nipponense. The methodology involved performing long-read and next generation transcriptome sequencing of genes from the androgenic gland after eyestalk ablation. qPCR analysis revealed that the mRNA expression of Mn-IAG was significantly increased after ablation of both the single-side (SS) and double-side (DS) eyestalk, compared with the control group (CG). The long-read transcriptome generated 49,840 non-redundant transcripts. A total of 1319, 2092 and 4351 differentially expressed genes (DEGs) were identified between CG versus SS, SS versus DS and CG versus DS, respectively. These data indicated that ablation of the double-sided eyestalk played stronger regulatory roles than the single-side ablation on male sexual development in M. nipponense. This was consistent with the qPCR analysis. Cell Cycle, Cellular Senescence, Oxidative Phosphorylation, Glycolysis/Gluconeogenesis and Steroid Hormone Biosynthesis were the primary enriched metabolic pathways in all three comparisons, and the important genes from these metabolic pathways were also selected. qPCR permitted secondary confirmation of ten DEGs identified through RNA-seq. RNAi-mediated silencing analyses of Hydroxysteroid dehydrogenase like 1 (HSDL1) revealed that HSDL1 has a positive regulatory effect on testes development. This study provides valuable insight into male sexual development in M. nipponense, including metabolic pathways and genes, paving the way for advanced studies on male sexual development in this species and in other crustaceans.
format	article
author	Shubo Jin Yin Fu Yuning Hu Hongtuo Fu Sufei Jiang Yiwei Xiong Hui Qiao Wenyi Zhang Yongsheng Gong Yan Wu
author_facet	Shubo Jin Yin Fu Yuning Hu Hongtuo Fu Sufei Jiang Yiwei Xiong Hui Qiao Wenyi Zhang Yongsheng Gong Yan Wu
author_sort	Shubo Jin
title	Identification of candidate genes from androgenic gland in Macrobrachium nipponense regulated by eyestalk ablation
title_short	Identification of candidate genes from androgenic gland in Macrobrachium nipponense regulated by eyestalk ablation
title_full	Identification of candidate genes from androgenic gland in Macrobrachium nipponense regulated by eyestalk ablation
title_fullStr	Identification of candidate genes from androgenic gland in Macrobrachium nipponense regulated by eyestalk ablation
title_full_unstemmed	Identification of candidate genes from androgenic gland in Macrobrachium nipponense regulated by eyestalk ablation
title_sort	identification of candidate genes from androgenic gland in macrobrachium nipponense regulated by eyestalk ablation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/94b5c56ae8694c9186308e880760a42d
work_keys_str_mv	AT shubojin identificationofcandidategenesfromandrogenicglandinmacrobrachiumnipponenseregulatedbyeyestalkablation AT yinfu identificationofcandidategenesfromandrogenicglandinmacrobrachiumnipponenseregulatedbyeyestalkablation AT yuninghu identificationofcandidategenesfromandrogenicglandinmacrobrachiumnipponenseregulatedbyeyestalkablation AT hongtuofu identificationofcandidategenesfromandrogenicglandinmacrobrachiumnipponenseregulatedbyeyestalkablation AT sufeijiang identificationofcandidategenesfromandrogenicglandinmacrobrachiumnipponenseregulatedbyeyestalkablation AT yiweixiong identificationofcandidategenesfromandrogenicglandinmacrobrachiumnipponenseregulatedbyeyestalkablation AT huiqiao identificationofcandidategenesfromandrogenicglandinmacrobrachiumnipponenseregulatedbyeyestalkablation AT wenyizhang identificationofcandidategenesfromandrogenicglandinmacrobrachiumnipponenseregulatedbyeyestalkablation AT yongshenggong identificationofcandidategenesfromandrogenicglandinmacrobrachiumnipponenseregulatedbyeyestalkablation AT yanwu identificationofcandidategenesfromandrogenicglandinmacrobrachiumnipponenseregulatedbyeyestalkablation
_version_	1718376986549682176

Identification of candidate genes from androgenic gland in Macrobrachium nipponense regulated by eyestalk ablation

Ejemplares similares