Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes

Abstract Electrical and/or electromechanical stimulation has been shown to play a significant role in regenerating various functionalities in soft tissues, such as tendons, muscles, and nerves. In this work, we investigate the piezoelectric polymer polyvinylidene fluoride (PVDF) as a potential subst...

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Autores principales:	Marcus Hoop, Xiang-Zhong Chen, Aldo Ferrari, Fajer Mushtaq, Gagik Ghazaryan, Theo Tervoort, Dimos Poulikakos, Bradley Nelson, Salvador Pané
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/94bba6576aa544388599d566c5e44e41
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spelling	oai:doaj.org-article:94bba6576aa544388599d566c5e44e412021-12-02T15:05:14ZUltrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes10.1038/s41598-017-03992-32045-2322https://doaj.org/article/94bba6576aa544388599d566c5e44e412017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03992-3https://doaj.org/toc/2045-2322Abstract Electrical and/or electromechanical stimulation has been shown to play a significant role in regenerating various functionalities in soft tissues, such as tendons, muscles, and nerves. In this work, we investigate the piezoelectric polymer polyvinylidene fluoride (PVDF) as a potential substrate for wireless neuronal differentiation. Piezoelectric PVDF enables generation of electrical charges on its surface upon acoustic stimulation, inducing neuritogenesis of PC12 cells. We demonstrate that the effect of pure piezoelectric stimulation on neurite generation in PC12 cells is comparable to the ones induced by neuronal growth factor (NGF). In inhibitor experiments, our results indicate that dynamic stimulation of PVDF by ultrasonic (US) waves activates calcium channels, thus inducing the generation of neurites via a cyclic adenosine monophosphate (cAMP)-dependent pathway. This mechanism is independent from the well-studied NGF induced mitogen-activated protein kinases/extracellular signal-regulated kinases (MAPK/ERK) pathway. The use of US, in combination with piezoelectric polymers, is advantageous since focused power transmission can occur deep into biological tissues, which holds great promise for the development of non-invasive neuroregenerative devices.Marcus HoopXiang-Zhong ChenAldo FerrariFajer MushtaqGagik GhazaryanTheo TervoortDimos PoulikakosBradley NelsonSalvador PanéNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcus Hoop Xiang-Zhong Chen Aldo Ferrari Fajer Mushtaq Gagik Ghazaryan Theo Tervoort Dimos Poulikakos Bradley Nelson Salvador Pané Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes
description	Abstract Electrical and/or electromechanical stimulation has been shown to play a significant role in regenerating various functionalities in soft tissues, such as tendons, muscles, and nerves. In this work, we investigate the piezoelectric polymer polyvinylidene fluoride (PVDF) as a potential substrate for wireless neuronal differentiation. Piezoelectric PVDF enables generation of electrical charges on its surface upon acoustic stimulation, inducing neuritogenesis of PC12 cells. We demonstrate that the effect of pure piezoelectric stimulation on neurite generation in PC12 cells is comparable to the ones induced by neuronal growth factor (NGF). In inhibitor experiments, our results indicate that dynamic stimulation of PVDF by ultrasonic (US) waves activates calcium channels, thus inducing the generation of neurites via a cyclic adenosine monophosphate (cAMP)-dependent pathway. This mechanism is independent from the well-studied NGF induced mitogen-activated protein kinases/extracellular signal-regulated kinases (MAPK/ERK) pathway. The use of US, in combination with piezoelectric polymers, is advantageous since focused power transmission can occur deep into biological tissues, which holds great promise for the development of non-invasive neuroregenerative devices.
format	article
author	Marcus Hoop Xiang-Zhong Chen Aldo Ferrari Fajer Mushtaq Gagik Ghazaryan Theo Tervoort Dimos Poulikakos Bradley Nelson Salvador Pané
author_facet	Marcus Hoop Xiang-Zhong Chen Aldo Ferrari Fajer Mushtaq Gagik Ghazaryan Theo Tervoort Dimos Poulikakos Bradley Nelson Salvador Pané
author_sort	Marcus Hoop
title	Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes
title_short	Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes
title_full	Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes
title_fullStr	Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes
title_full_unstemmed	Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes
title_sort	ultrasound-mediated piezoelectric differentiation of neuron-like pc12 cells on pvdf membranes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/94bba6576aa544388599d566c5e44e41
work_keys_str_mv	AT marcushoop ultrasoundmediatedpiezoelectricdifferentiationofneuronlikepc12cellsonpvdfmembranes AT xiangzhongchen ultrasoundmediatedpiezoelectricdifferentiationofneuronlikepc12cellsonpvdfmembranes AT aldoferrari ultrasoundmediatedpiezoelectricdifferentiationofneuronlikepc12cellsonpvdfmembranes AT fajermushtaq ultrasoundmediatedpiezoelectricdifferentiationofneuronlikepc12cellsonpvdfmembranes AT gagikghazaryan ultrasoundmediatedpiezoelectricdifferentiationofneuronlikepc12cellsonpvdfmembranes AT theotervoort ultrasoundmediatedpiezoelectricdifferentiationofneuronlikepc12cellsonpvdfmembranes AT dimospoulikakos ultrasoundmediatedpiezoelectricdifferentiationofneuronlikepc12cellsonpvdfmembranes AT bradleynelson ultrasoundmediatedpiezoelectricdifferentiationofneuronlikepc12cellsonpvdfmembranes AT salvadorpane ultrasoundmediatedpiezoelectricdifferentiationofneuronlikepc12cellsonpvdfmembranes
_version_	1718388906937810944

Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes

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