Fabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated RAFT polymerization

Fabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated RAFT polymerization

Bailiang Wang,1,2 Zi Ye,1 Yihong Tang,1 Yuemei Han,1 Quankui Lin,1,2 Huihua Liu,2 Hao Chen,1,2 Kaihui Nan1,2 1School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 2Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, People&am...

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Autores principales: Wang B, Ye Z, Tang Y, Han Y, Lin Q, Liu H, Chen H, Nan K
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2016
Materias:
Anti-fouling
Poly(dimethylsiloxane)
Intraocular lenses
Endophthalmitis
Biofilm;
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/94c5ece123264623857375edb59b320b
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spelling oai:doaj.org-article:94c5ece123264623857375edb59b320b2021-12-02T05:08:58ZFabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated RAFT polymerization1178-2013https://doaj.org/article/94c5ece123264623857375edb59b320b2016-12-01T00:00:00Zhttps://www.dovepress.com/fabrication-of-nonfouling-bactericidal-and-bacteria-corpse-release-mu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Bailiang Wang,1,2 Zi Ye,1 Yihong Tang,1 Yuemei Han,1 Quankui Lin,1,2 Huihua Liu,2 Hao Chen,1,2 Kaihui Nan1,2 1School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 2Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, People’s Republic of China Abstract: Infections after surgery or endophthalmitis are potentially blinding complications caused by bacterial adhesion and subsequent biofilm formation on the intraocular lens. Neither single-function anti-adhesion surface nor contacting killing surface can exhibit ideal antibacterial function. In this work, a novel (2-(dimethylamino)-ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) (p (DMAEMA-co-MPC)) brush was synthesized by “grafting from” method through reversible–addition fragmentation chain transfer polymerization. 1-Bromoheptane was used to quaternize the p (DMAEMA-co-MPC) brush coating and to endow the surface with bactericidal function. The success of the surface functionalization was confirmed by atomic force microscopy, water contact angle, and spectroscopic ellipsometry. The quaternary ammonium salt units were employed as efficient disinfection that can eliminate bacteria through contact killing, whereas the 2-methacryloyloxyethyl phosphorylcholine units were introduced to suppress unwanted nonspecific adsorption. The functionalized poly(dimethyl siloxane) surfaces showed efficiency in reducing bovine serum albumin adsorption and in inhibiting bacteria adhesion and biofilm formation. The copolymer brushes also demonstrated excellent bactericidal function against gram-positive (Staphylococcus aureus) bacteria measured by bacteria live/dead staining and shake-flask culture methods. The surface biocompatibility was evaluated by morphology and activity measurement with human lens epithelial cells in vitro. The achievement of the p (DMAEMA+-co-MPC) copolymer brush coating with nonfouling, bactericidal, and bacteria corpse release properties can be used to modify intraocular lenses. Keywords: anti-fouling, poly(dimethylsiloxane), intraocular lenses, endophthalmitis, biofilmsWang BYe ZTang YHan YLin QLiu HChen HNan KDove Medical PressarticleAnti-foulingPoly(dimethylsiloxane)Intraocular lensesEndophthalmitisBiofilm;Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 111-125 (2016)
institution DOAJ
collection DOAJ
language EN
topic Anti-fouling
Poly(dimethylsiloxane)
Intraocular lenses
Endophthalmitis
Biofilm;
Medicine (General)
R5-920
spellingShingle Anti-fouling
Poly(dimethylsiloxane)
Intraocular lenses
Endophthalmitis
Biofilm;
Medicine (General)
R5-920
Wang B
Ye Z
Tang Y
Han Y
Lin Q
Liu H
Chen H
Nan K
Fabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated RAFT polymerization
description Bailiang Wang,1,2 Zi Ye,1 Yihong Tang,1 Yuemei Han,1 Quankui Lin,1,2 Huihua Liu,2 Hao Chen,1,2 Kaihui Nan1,2 1School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, 2Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, People’s Republic of China Abstract: Infections after surgery or endophthalmitis are potentially blinding complications caused by bacterial adhesion and subsequent biofilm formation on the intraocular lens. Neither single-function anti-adhesion surface nor contacting killing surface can exhibit ideal antibacterial function. In this work, a novel (2-(dimethylamino)-ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) (p (DMAEMA-co-MPC)) brush was synthesized by “grafting from” method through reversible–addition fragmentation chain transfer polymerization. 1-Bromoheptane was used to quaternize the p (DMAEMA-co-MPC) brush coating and to endow the surface with bactericidal function. The success of the surface functionalization was confirmed by atomic force microscopy, water contact angle, and spectroscopic ellipsometry. The quaternary ammonium salt units were employed as efficient disinfection that can eliminate bacteria through contact killing, whereas the 2-methacryloyloxyethyl phosphorylcholine units were introduced to suppress unwanted nonspecific adsorption. The functionalized poly(dimethyl siloxane) surfaces showed efficiency in reducing bovine serum albumin adsorption and in inhibiting bacteria adhesion and biofilm formation. The copolymer brushes also demonstrated excellent bactericidal function against gram-positive (Staphylococcus aureus) bacteria measured by bacteria live/dead staining and shake-flask culture methods. The surface biocompatibility was evaluated by morphology and activity measurement with human lens epithelial cells in vitro. The achievement of the p (DMAEMA+-co-MPC) copolymer brush coating with nonfouling, bactericidal, and bacteria corpse release properties can be used to modify intraocular lenses. Keywords: anti-fouling, poly(dimethylsiloxane), intraocular lenses, endophthalmitis, biofilms
format article
author Wang B
Ye Z
Tang Y
Han Y
Lin Q
Liu H
Chen H
Nan K
author_facet Wang B
Ye Z
Tang Y
Han Y
Lin Q
Liu H
Chen H
Nan K
author_sort Wang B
title Fabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated RAFT polymerization
title_short Fabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated RAFT polymerization
title_full Fabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated RAFT polymerization
title_fullStr Fabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated RAFT polymerization
title_full_unstemmed Fabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated RAFT polymerization
title_sort fabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated raft polymerization
publisher Dove Medical Press
publishDate 2016
url https://doaj.org/article/94c5ece123264623857375edb59b320b
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