Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH

Abstract Mucins, the main component of the mucus secretions of goblet and epithelial cells, are known for exhibiting a different behaviour in accordance with their surrounding environment (i.e. among others the environmental pH), which induces a drastic change in their measured mechanical properties...

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Autores principales:	Maria Sumarokova, Jagoba Iturri, Andreas Weber, Maria Maares, Claudia Keil, Hajo Haase, José Luis Toca-Herrera
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/94c701e22829498bbfaf524bdc399765
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spelling	oai:doaj.org-article:94c701e22829498bbfaf524bdc3997652021-12-02T11:41:13ZInfluencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH10.1038/s41598-018-28047-z2045-2322https://doaj.org/article/94c701e22829498bbfaf524bdc3997652018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28047-zhttps://doaj.org/toc/2045-2322Abstract Mucins, the main component of the mucus secretions of goblet and epithelial cells, are known for exhibiting a different behaviour in accordance with their surrounding environment (i.e. among others the environmental pH), which induces a drastic change in their measured mechanical properties. In this work, we have first employed Atomic Force Microscopy (AFM) in Force Spectroscopy mode to evaluate the adhesion of porcine mucin films at the nanoscale, and the changes caused in this particular factor by a pH variation between 7.0 and 4.0, both quite common values in biological conditions. Measurements also involved additional varying factors such as the indenting tip chemistry (hydrophobic vs hydrophilic), its residence time on the measured film (0, 1 and/or 2 seconds), and increasing pulling rates (ranging from 0.1 up to 10 µm/s). A second approach regarded the macroscale behaviour of the films, due to their potential applicability in the development of a new set of stimuli-responsive biomaterials. This was possible by means of complementary Wilhelmy plate method (to test the wetting properties) and cell proliferation studies on films previously exposed to the corresponding pH solution. According to our results, treatment with lowest pH (4.0) provides porcine mucin with a more hydrophilic character, showing a much stronger adhesion for analogous chemistries, as well as enhanced capability for cell attachment and proliferation, which opens new pathways for their future use and consideration as scaffold-forming material.Maria SumarokovaJagoba IturriAndreas WeberMaria MaaresClaudia KeilHajo HaaseJosé Luis Toca-HerreraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Sumarokova Jagoba Iturri Andreas Weber Maria Maares Claudia Keil Hajo Haase José Luis Toca-Herrera Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH
description	Abstract Mucins, the main component of the mucus secretions of goblet and epithelial cells, are known for exhibiting a different behaviour in accordance with their surrounding environment (i.e. among others the environmental pH), which induces a drastic change in their measured mechanical properties. In this work, we have first employed Atomic Force Microscopy (AFM) in Force Spectroscopy mode to evaluate the adhesion of porcine mucin films at the nanoscale, and the changes caused in this particular factor by a pH variation between 7.0 and 4.0, both quite common values in biological conditions. Measurements also involved additional varying factors such as the indenting tip chemistry (hydrophobic vs hydrophilic), its residence time on the measured film (0, 1 and/or 2 seconds), and increasing pulling rates (ranging from 0.1 up to 10 µm/s). A second approach regarded the macroscale behaviour of the films, due to their potential applicability in the development of a new set of stimuli-responsive biomaterials. This was possible by means of complementary Wilhelmy plate method (to test the wetting properties) and cell proliferation studies on films previously exposed to the corresponding pH solution. According to our results, treatment with lowest pH (4.0) provides porcine mucin with a more hydrophilic character, showing a much stronger adhesion for analogous chemistries, as well as enhanced capability for cell attachment and proliferation, which opens new pathways for their future use and consideration as scaffold-forming material.
format	article
author	Maria Sumarokova Jagoba Iturri Andreas Weber Maria Maares Claudia Keil Hajo Haase José Luis Toca-Herrera
author_facet	Maria Sumarokova Jagoba Iturri Andreas Weber Maria Maares Claudia Keil Hajo Haase José Luis Toca-Herrera
author_sort	Maria Sumarokova
title	Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH
title_short	Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH
title_full	Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH
title_fullStr	Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH
title_full_unstemmed	Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH
title_sort	influencing the adhesion properties and wettability of mucin protein films by variation of the environmental ph
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/94c701e22829498bbfaf524bdc399765
work_keys_str_mv	AT mariasumarokova influencingtheadhesionpropertiesandwettabilityofmucinproteinfilmsbyvariationoftheenvironmentalph AT jagobaiturri influencingtheadhesionpropertiesandwettabilityofmucinproteinfilmsbyvariationoftheenvironmentalph AT andreasweber influencingtheadhesionpropertiesandwettabilityofmucinproteinfilmsbyvariationoftheenvironmentalph AT mariamaares influencingtheadhesionpropertiesandwettabilityofmucinproteinfilmsbyvariationoftheenvironmentalph AT claudiakeil influencingtheadhesionpropertiesandwettabilityofmucinproteinfilmsbyvariationoftheenvironmentalph AT hajohaase influencingtheadhesionpropertiesandwettabilityofmucinproteinfilmsbyvariationoftheenvironmentalph AT joseluistocaherrera influencingtheadhesionpropertiesandwettabilityofmucinproteinfilmsbyvariationoftheenvironmentalph
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Influencing the adhesion properties and wettability of mucin protein films by variation of the environmental pH

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