Transcriptome of extracellular vesicles released by hepatocytes.

The discovery that the cells communicate through emission of vesicles has opened new opportunities for better understanding of physiological and pathological mechanisms. This discovery also provides a novel source for non-invasive disease biomarker research. Our group has previously reported that he...

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Autores principales:	Felix Royo, Karin Schlangen, Laura Palomo, Esperanza Gonzalez, Javier Conde-Vancells, Agustin Berisa, Ana M Aransay, Juan M Falcon-Perez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/94c885f172574df3846c91714908e7f3
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spelling	oai:doaj.org-article:94c885f172574df3846c91714908e7f32021-11-18T07:37:49ZTranscriptome of extracellular vesicles released by hepatocytes.1932-620310.1371/journal.pone.0068693https://doaj.org/article/94c885f172574df3846c91714908e7f32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874726/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The discovery that the cells communicate through emission of vesicles has opened new opportunities for better understanding of physiological and pathological mechanisms. This discovery also provides a novel source for non-invasive disease biomarker research. Our group has previously reported that hepatocytes release extracellular vesicles with protein content reflecting the cell-type of origin. Here, we show that the extracellular vesicles released by hepatocytes also carry RNA. We report the messenger RNA composition of extracellular vesicles released in two non-tumoral hepatic models: primary culture of rat hepatocytes and a progenitor cell line obtained from a mouse foetal liver. We describe different subpopulations of extracellular vesicles with different densities and protein and RNA content. We also show that the RNA cargo of extracellular vesicles released by primary hepatocytes can be transferred to rat liver stellate-like cells and promote their activation. Finally, we provide in vitro and in vivo evidence that liver-damaging drugs galactosamine, acetaminophen, and diclofenac modify the RNA content of these vesicles. To summarize, we show that the extracellular vesicles secreted by hepatocytes contain various RNAs. These vesicles, likely to be involved in the activation of stellate cells, might become a new source for non-invasive identification of the liver toxicity markers.Felix RoyoKarin SchlangenLaura PalomoEsperanza GonzalezJavier Conde-VancellsAgustin BerisaAna M AransayJuan M Falcon-PerezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68693 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Felix Royo Karin Schlangen Laura Palomo Esperanza Gonzalez Javier Conde-Vancells Agustin Berisa Ana M Aransay Juan M Falcon-Perez Transcriptome of extracellular vesicles released by hepatocytes.
description	The discovery that the cells communicate through emission of vesicles has opened new opportunities for better understanding of physiological and pathological mechanisms. This discovery also provides a novel source for non-invasive disease biomarker research. Our group has previously reported that hepatocytes release extracellular vesicles with protein content reflecting the cell-type of origin. Here, we show that the extracellular vesicles released by hepatocytes also carry RNA. We report the messenger RNA composition of extracellular vesicles released in two non-tumoral hepatic models: primary culture of rat hepatocytes and a progenitor cell line obtained from a mouse foetal liver. We describe different subpopulations of extracellular vesicles with different densities and protein and RNA content. We also show that the RNA cargo of extracellular vesicles released by primary hepatocytes can be transferred to rat liver stellate-like cells and promote their activation. Finally, we provide in vitro and in vivo evidence that liver-damaging drugs galactosamine, acetaminophen, and diclofenac modify the RNA content of these vesicles. To summarize, we show that the extracellular vesicles secreted by hepatocytes contain various RNAs. These vesicles, likely to be involved in the activation of stellate cells, might become a new source for non-invasive identification of the liver toxicity markers.
format	article
author	Felix Royo Karin Schlangen Laura Palomo Esperanza Gonzalez Javier Conde-Vancells Agustin Berisa Ana M Aransay Juan M Falcon-Perez
author_facet	Felix Royo Karin Schlangen Laura Palomo Esperanza Gonzalez Javier Conde-Vancells Agustin Berisa Ana M Aransay Juan M Falcon-Perez
author_sort	Felix Royo
title	Transcriptome of extracellular vesicles released by hepatocytes.
title_short	Transcriptome of extracellular vesicles released by hepatocytes.
title_full	Transcriptome of extracellular vesicles released by hepatocytes.
title_fullStr	Transcriptome of extracellular vesicles released by hepatocytes.
title_full_unstemmed	Transcriptome of extracellular vesicles released by hepatocytes.
title_sort	transcriptome of extracellular vesicles released by hepatocytes.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/94c885f172574df3846c91714908e7f3
work_keys_str_mv	AT felixroyo transcriptomeofextracellularvesiclesreleasedbyhepatocytes AT karinschlangen transcriptomeofextracellularvesiclesreleasedbyhepatocytes AT laurapalomo transcriptomeofextracellularvesiclesreleasedbyhepatocytes AT esperanzagonzalez transcriptomeofextracellularvesiclesreleasedbyhepatocytes AT javiercondevancells transcriptomeofextracellularvesiclesreleasedbyhepatocytes AT agustinberisa transcriptomeofextracellularvesiclesreleasedbyhepatocytes AT anamaransay transcriptomeofextracellularvesiclesreleasedbyhepatocytes AT juanmfalconperez transcriptomeofextracellularvesiclesreleasedbyhepatocytes
_version_	1718423176052998144

Transcriptome of extracellular vesicles released by hepatocytes.

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