Meta-analysis using a novel database, miRStress, reveals miRNAs that are frequently associated with the radiation and hypoxia stress-responses.

Organisms are often exposed to environmental pressures that affect homeostasis, so it is important to understand the biological basis of stress-response. Various biological mechanisms have evolved to help cells cope with potentially cytotoxic changes in their environment. miRNAs are small non-coding...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Laura Ann Jacobs, Findlay Bewicke-Copley, Mark Graham Poolman, Ryan Charles Pink, Laura Ann Mulcahy, Isabel Baker, Ellie-May Beaman, Travis Brooks, Daniel Paul Caley, William Cowling, James Michael Stevenson Currie, Jessica Horsburgh, Lottie Kenehan, Emma Keyes, Daniel Leite, Davide Massa, Adam McDermott-Rouse, Priya Samuel, Hannah Wood, Munira Kadhim, David Raul Francisco Carter
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2013
Materias:
R
Q
Acceso en línea:https://doaj.org/article/94d1d6f99caa459e962d88eb27e9de71
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:94d1d6f99caa459e962d88eb27e9de71
record_format dspace
spelling oai:doaj.org-article:94d1d6f99caa459e962d88eb27e9de712021-11-18T08:46:22ZMeta-analysis using a novel database, miRStress, reveals miRNAs that are frequently associated with the radiation and hypoxia stress-responses.1932-620310.1371/journal.pone.0080844https://doaj.org/article/94d1d6f99caa459e962d88eb27e9de712013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24244721/?tool=EBIhttps://doaj.org/toc/1932-6203Organisms are often exposed to environmental pressures that affect homeostasis, so it is important to understand the biological basis of stress-response. Various biological mechanisms have evolved to help cells cope with potentially cytotoxic changes in their environment. miRNAs are small non-coding RNAs which are able to regulate mRNA stability. It has been suggested that miRNAs may tip the balance between continued cytorepair and induction of apoptosis in response to stress. There is a wealth of data in the literature showing the effect of environmental stress on miRNAs, but it is scattered in a large number of disparate publications. Meta-analyses of this data would produce added insight into the molecular mechanisms of stress-response. To facilitate this we created and manually curated the miRStress database, which describes the changes in miRNA levels following an array of stress types in eukaryotic cells. Here we describe this database and validate the miRStress tool for analysing miRNAs that are regulated by stress. To validate the database we performed a cross-species analysis to identify miRNAs that respond to radiation. The analysis tool confirms miR-21 and miR-34a as frequently deregulated in response to radiation, but also identifies novel candidates as potentially important players in this stress response, including miR-15b, miR-19b, and miR-106a. Similarly, we used the miRStress tool to analyse hypoxia-responsive miRNAs. The most frequently deregulated miRNAs were miR-210 and miR-21, as expected. Several other miRNAs were also found to be associated with hypoxia, including miR-181b, miR-26a/b, miR-106a, miR-213 and miR-192. Therefore the miRStress tool has identified miRNAs with hitherto unknown or under-appreciated roles in the response to specific stress types. The miRStress tool, which can be used to uncover new insight into the biological roles of miRNAs, and also has the potential to unearth potential biomarkers for therapeutic response, is freely available at http://mudshark.brookes.ac.uk/MirStress.Laura Ann JacobsFindlay Bewicke-CopleyMark Graham PoolmanRyan Charles PinkRyan Charles PinkLaura Ann MulcahyIsabel BakerEllie-May BeamanTravis BrooksDaniel Paul CaleyWilliam CowlingJames Michael Stevenson CurrieJessica HorsburghLottie KenehanEmma KeyesDaniel LeiteDavide MassaAdam McDermott-RousePriya SamuelHannah WoodMunira KadhimDavid Raul Francisco CarterPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e80844 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Laura Ann Jacobs
Findlay Bewicke-Copley
Mark Graham Poolman
Ryan Charles Pink
Ryan Charles Pink
Laura Ann Mulcahy
Isabel Baker
Ellie-May Beaman
Travis Brooks
Daniel Paul Caley
William Cowling
James Michael Stevenson Currie
Jessica Horsburgh
Lottie Kenehan
Emma Keyes
Daniel Leite
Davide Massa
Adam McDermott-Rouse
Priya Samuel
Hannah Wood
Munira Kadhim
David Raul Francisco Carter
Meta-analysis using a novel database, miRStress, reveals miRNAs that are frequently associated with the radiation and hypoxia stress-responses.
description Organisms are often exposed to environmental pressures that affect homeostasis, so it is important to understand the biological basis of stress-response. Various biological mechanisms have evolved to help cells cope with potentially cytotoxic changes in their environment. miRNAs are small non-coding RNAs which are able to regulate mRNA stability. It has been suggested that miRNAs may tip the balance between continued cytorepair and induction of apoptosis in response to stress. There is a wealth of data in the literature showing the effect of environmental stress on miRNAs, but it is scattered in a large number of disparate publications. Meta-analyses of this data would produce added insight into the molecular mechanisms of stress-response. To facilitate this we created and manually curated the miRStress database, which describes the changes in miRNA levels following an array of stress types in eukaryotic cells. Here we describe this database and validate the miRStress tool for analysing miRNAs that are regulated by stress. To validate the database we performed a cross-species analysis to identify miRNAs that respond to radiation. The analysis tool confirms miR-21 and miR-34a as frequently deregulated in response to radiation, but also identifies novel candidates as potentially important players in this stress response, including miR-15b, miR-19b, and miR-106a. Similarly, we used the miRStress tool to analyse hypoxia-responsive miRNAs. The most frequently deregulated miRNAs were miR-210 and miR-21, as expected. Several other miRNAs were also found to be associated with hypoxia, including miR-181b, miR-26a/b, miR-106a, miR-213 and miR-192. Therefore the miRStress tool has identified miRNAs with hitherto unknown or under-appreciated roles in the response to specific stress types. The miRStress tool, which can be used to uncover new insight into the biological roles of miRNAs, and also has the potential to unearth potential biomarkers for therapeutic response, is freely available at http://mudshark.brookes.ac.uk/MirStress.
format article
author Laura Ann Jacobs
Findlay Bewicke-Copley
Mark Graham Poolman
Ryan Charles Pink
Ryan Charles Pink
Laura Ann Mulcahy
Isabel Baker
Ellie-May Beaman
Travis Brooks
Daniel Paul Caley
William Cowling
James Michael Stevenson Currie
Jessica Horsburgh
Lottie Kenehan
Emma Keyes
Daniel Leite
Davide Massa
Adam McDermott-Rouse
Priya Samuel
Hannah Wood
Munira Kadhim
David Raul Francisco Carter
author_facet Laura Ann Jacobs
Findlay Bewicke-Copley
Mark Graham Poolman
Ryan Charles Pink
Ryan Charles Pink
Laura Ann Mulcahy
Isabel Baker
Ellie-May Beaman
Travis Brooks
Daniel Paul Caley
William Cowling
James Michael Stevenson Currie
Jessica Horsburgh
Lottie Kenehan
Emma Keyes
Daniel Leite
Davide Massa
Adam McDermott-Rouse
Priya Samuel
Hannah Wood
Munira Kadhim
David Raul Francisco Carter
author_sort Laura Ann Jacobs
title Meta-analysis using a novel database, miRStress, reveals miRNAs that are frequently associated with the radiation and hypoxia stress-responses.
title_short Meta-analysis using a novel database, miRStress, reveals miRNAs that are frequently associated with the radiation and hypoxia stress-responses.
title_full Meta-analysis using a novel database, miRStress, reveals miRNAs that are frequently associated with the radiation and hypoxia stress-responses.
title_fullStr Meta-analysis using a novel database, miRStress, reveals miRNAs that are frequently associated with the radiation and hypoxia stress-responses.
title_full_unstemmed Meta-analysis using a novel database, miRStress, reveals miRNAs that are frequently associated with the radiation and hypoxia stress-responses.
title_sort meta-analysis using a novel database, mirstress, reveals mirnas that are frequently associated with the radiation and hypoxia stress-responses.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/94d1d6f99caa459e962d88eb27e9de71
work_keys_str_mv AT lauraannjacobs metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT findlaybewickecopley metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT markgrahampoolman metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT ryancharlespink metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT ryancharlespink metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT lauraannmulcahy metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT isabelbaker metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT elliemaybeaman metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT travisbrooks metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT danielpaulcaley metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT williamcowling metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT jamesmichaelstevensoncurrie metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT jessicahorsburgh metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT lottiekenehan metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT emmakeyes metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT danielleite metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT davidemassa metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT adammcdermottrouse metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT priyasamuel metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT hannahwood metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT munirakadhim metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
AT davidraulfranciscocarter metaanalysisusinganoveldatabasemirstressrevealsmirnasthatarefrequentlyassociatedwiththeradiationandhypoxiastressresponses
_version_ 1718421336475303936