PHAIR: a biosensor for pH measurement in air–liquid interface cell culture

Abstract In many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available pH sensors are difficult to integrate...

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Autores principales:	Mohammadhossein Dabaghi, Neda Saraei, Gang Xu, Abiram Chandiramohan, Jonas Yeung, Jenny P. Nguyen, Milica Vukmirovic, Ponnambalam Ravi Selvaganapathy, Jeremy A. Hirota
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/94d3ed8060724bb2aef7031770f38b36
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spelling	oai:doaj.org-article:94d3ed8060724bb2aef7031770f38b362021-12-02T14:26:47ZPHAIR: a biosensor for pH measurement in air–liquid interface cell culture10.1038/s41598-021-83189-x2045-2322https://doaj.org/article/94d3ed8060724bb2aef7031770f38b362021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83189-xhttps://doaj.org/toc/2045-2322Abstract In many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available pH sensors are difficult to integrate into a small-scale cell culture system due to their size and are not cost-effective for disposable use. We made PHAIR—a new pH sensor that uses a micro-wire format to measure pH in vitro human airway cell culture. Tungsten micro-wires were used as the working electrodes, and silver micro-wires with a silver/silver chloride coating were used as a pseudo reference electrode. pH sensitivity, in a wide and narrow range, and stability of these sensors were tested in common standard buffer solutions as well as in culture media of human airway epithelial cells grown at the air–liquid interface in a 24 well cell culture plate. When measuring the pH of cells grown under basal and challenge conditions using PHAIR, cell viability and cytokine responses were not affected. Our results confirm that micro-wire-based sensors have the capacity for miniaturization and detection of diverse ions while maintaining sensitivity. This suggests the broad application of PHAIR in various biological experimental settings.Mohammadhossein DabaghiNeda SaraeiGang XuAbiram ChandiramohanJonas YeungJenny P. NguyenMilica VukmirovicPonnambalam Ravi SelvaganapathyJeremy A. HirotaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammadhossein Dabaghi Neda Saraei Gang Xu Abiram Chandiramohan Jonas Yeung Jenny P. Nguyen Milica Vukmirovic Ponnambalam Ravi Selvaganapathy Jeremy A. Hirota PHAIR: a biosensor for pH measurement in air–liquid interface cell culture
description	Abstract In many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available pH sensors are difficult to integrate into a small-scale cell culture system due to their size and are not cost-effective for disposable use. We made PHAIR—a new pH sensor that uses a micro-wire format to measure pH in vitro human airway cell culture. Tungsten micro-wires were used as the working electrodes, and silver micro-wires with a silver/silver chloride coating were used as a pseudo reference electrode. pH sensitivity, in a wide and narrow range, and stability of these sensors were tested in common standard buffer solutions as well as in culture media of human airway epithelial cells grown at the air–liquid interface in a 24 well cell culture plate. When measuring the pH of cells grown under basal and challenge conditions using PHAIR, cell viability and cytokine responses were not affected. Our results confirm that micro-wire-based sensors have the capacity for miniaturization and detection of diverse ions while maintaining sensitivity. This suggests the broad application of PHAIR in various biological experimental settings.
format	article
author	Mohammadhossein Dabaghi Neda Saraei Gang Xu Abiram Chandiramohan Jonas Yeung Jenny P. Nguyen Milica Vukmirovic Ponnambalam Ravi Selvaganapathy Jeremy A. Hirota
author_facet	Mohammadhossein Dabaghi Neda Saraei Gang Xu Abiram Chandiramohan Jonas Yeung Jenny P. Nguyen Milica Vukmirovic Ponnambalam Ravi Selvaganapathy Jeremy A. Hirota
author_sort	Mohammadhossein Dabaghi
title	PHAIR: a biosensor for pH measurement in air–liquid interface cell culture
title_short	PHAIR: a biosensor for pH measurement in air–liquid interface cell culture
title_full	PHAIR: a biosensor for pH measurement in air–liquid interface cell culture
title_fullStr	PHAIR: a biosensor for pH measurement in air–liquid interface cell culture
title_full_unstemmed	PHAIR: a biosensor for pH measurement in air–liquid interface cell culture
title_sort	phair: a biosensor for ph measurement in air–liquid interface cell culture
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/94d3ed8060724bb2aef7031770f38b36
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PHAIR: a biosensor for pH measurement in air–liquid interface cell culture

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