Comparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (Trionyx sinensis) Infected with Aeromonas hydrophila

Although hemorrhagic sepsis caused by Aeromonas hydrophila infection is the dominant disease in the aquaculture of Chinese soft-shelled turtle, information on its molecular pathology is seriously limited. In this study, ninety turtles intraperitoneally injected with A. hy...

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Autores principales:	Zhao Lv, Yazhou Hu, Jin Tan, Xiaoqing Wang, Xiaoyan Liu, Cong Zeng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Chinese soft-shelled turtle <i>Aeromonas hydrophila</i> hemorrhagic sepsis molecular immunopathogenesis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/94daca6c2af04f66affba6850451523e
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spelling	oai:doaj.org-article:94daca6c2af04f66affba6850451523e2021-11-25T16:48:16ZComparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (<i>Trionyx sinensis</i>) Infected with <i>Aeromonas hydrophila</i>10.3390/biology101112182079-7737https://doaj.org/article/94daca6c2af04f66affba6850451523e2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1218https://doaj.org/toc/2079-7737Although hemorrhagic sepsis caused by <i>Aeromonas hydrophila</i> infection is the dominant disease in the aquaculture of Chinese soft-shelled turtle, information on its molecular pathology is seriously limited. In this study, ninety turtles intraperitoneally injected with <i>A. hydrophila</i> exhibited two different phenotypes based on the pathological symptoms, referred to as active and inactive turtles. Comparative transcriptomes of liver and spleen from these two groups at 6, 24, and 72 h post-injection (hpi) were further analyzed. The results showed that cytokine–cytokine receptor interaction, PRRs mediated signaling pathway, apoptosis, and phagocytosis enriched in active and inactive turtles were significantly different. Pro-inflammatory cytokines, the TLR signaling pathway, NLR signaling pathway, and RLR signaling pathway mediating cytokine expression, and apoptosis-related genes, were significantly up-regulated in inactive turtles at the early stage (6 hpi). The significant up-regulation of phagocytosis-related genes occurred at 24 hpi in inactive turtles and relatively lagged behind those in active turtles. The anti-inflammatory cytokine, IL10, was significantly up-regulated during the tested periods (6, 24, and 72 hpi) in active turtles. These findings offer valuable information for the understanding of molecular immunopathogenesis after <i>A. hydrophila</i> infection, and facilitate further investigations on strategies against hemorrhagic sepsis in Chinese soft-shelled turtle <i>T. sinensis</i>.Zhao LvYazhou HuJin TanXiaoqing WangXiaoyan LiuCong ZengMDPI AGarticleChinese soft-shelled turtle<i>Aeromonas hydrophila</i>hemorrhagic sepsismolecular immunopathogenesisBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1218, p 1218 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chinese soft-shelled turtle <i>Aeromonas hydrophila</i> hemorrhagic sepsis molecular immunopathogenesis Biology (General) QH301-705.5
spellingShingle	Chinese soft-shelled turtle <i>Aeromonas hydrophila</i> hemorrhagic sepsis molecular immunopathogenesis Biology (General) QH301-705.5 Zhao Lv Yazhou Hu Jin Tan Xiaoqing Wang Xiaoyan Liu Cong Zeng Comparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (<i>Trionyx sinensis</i>) Infected with <i>Aeromonas hydrophila</i>
description	Although hemorrhagic sepsis caused by <i>Aeromonas hydrophila</i> infection is the dominant disease in the aquaculture of Chinese soft-shelled turtle, information on its molecular pathology is seriously limited. In this study, ninety turtles intraperitoneally injected with <i>A. hydrophila</i> exhibited two different phenotypes based on the pathological symptoms, referred to as active and inactive turtles. Comparative transcriptomes of liver and spleen from these two groups at 6, 24, and 72 h post-injection (hpi) were further analyzed. The results showed that cytokine–cytokine receptor interaction, PRRs mediated signaling pathway, apoptosis, and phagocytosis enriched in active and inactive turtles were significantly different. Pro-inflammatory cytokines, the TLR signaling pathway, NLR signaling pathway, and RLR signaling pathway mediating cytokine expression, and apoptosis-related genes, were significantly up-regulated in inactive turtles at the early stage (6 hpi). The significant up-regulation of phagocytosis-related genes occurred at 24 hpi in inactive turtles and relatively lagged behind those in active turtles. The anti-inflammatory cytokine, IL10, was significantly up-regulated during the tested periods (6, 24, and 72 hpi) in active turtles. These findings offer valuable information for the understanding of molecular immunopathogenesis after <i>A. hydrophila</i> infection, and facilitate further investigations on strategies against hemorrhagic sepsis in Chinese soft-shelled turtle <i>T. sinensis</i>.
format	article
author	Zhao Lv Yazhou Hu Jin Tan Xiaoqing Wang Xiaoyan Liu Cong Zeng
author_facet	Zhao Lv Yazhou Hu Jin Tan Xiaoqing Wang Xiaoyan Liu Cong Zeng
author_sort	Zhao Lv
title	Comparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (<i>Trionyx sinensis</i>) Infected with <i>Aeromonas hydrophila</i>
title_short	Comparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (<i>Trionyx sinensis</i>) Infected with <i>Aeromonas hydrophila</i>
title_full	Comparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (<i>Trionyx sinensis</i>) Infected with <i>Aeromonas hydrophila</i>
title_fullStr	Comparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (<i>Trionyx sinensis</i>) Infected with <i>Aeromonas hydrophila</i>
title_full_unstemmed	Comparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (<i>Trionyx sinensis</i>) Infected with <i>Aeromonas hydrophila</i>
title_sort	comparative transcriptome analysis reveals the molecular immunopathogenesis of chinese soft-shelled turtle (<i>trionyx sinensis</i>) infected with <i>aeromonas hydrophila</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/94daca6c2af04f66affba6850451523e
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Comparative Transcriptome Analysis Reveals the Molecular Immunopathogenesis of Chinese Soft-Shelled Turtle (<i>Trionyx sinensis</i>) Infected with <i>Aeromonas hydrophila</i>

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