Histone deacetylase HDA-4-mediated epigenetic regulation in space-flown C. elegans

Abstract Epigenetic changes during long-term spaceflight are beginning to be studied by NASA’s twin astronauts and other model organisms. Here, we evaluate the epigenetic regulation of gene expression in space-flown C. elegans by comparing wild type and histone deacetylase (hda)-4 mutants. Expressio...

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Autores principales:	Atsushi Higashitani, Toko Hashizume, Mai Takiura, Nahoko Higashitani, Mika Teranishi, Rika Oshima, Sachiko Yano, Kana Kuriyama, Akira Higashibata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biotechnology TP248.13-248.65 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/94dd2e3bfe9947d8accd09979545aa51
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spelling	oai:doaj.org-article:94dd2e3bfe9947d8accd09979545aa512021-12-02T16:38:50ZHistone deacetylase HDA-4-mediated epigenetic regulation in space-flown C. elegans10.1038/s41526-021-00163-72373-8065https://doaj.org/article/94dd2e3bfe9947d8accd09979545aa512021-09-01T00:00:00Zhttps://doi.org/10.1038/s41526-021-00163-7https://doaj.org/toc/2373-8065Abstract Epigenetic changes during long-term spaceflight are beginning to be studied by NASA’s twin astronauts and other model organisms. Here, we evaluate the epigenetic regulation of gene expression in space-flown C. elegans by comparing wild type and histone deacetylase (hda)-4 mutants. Expression levels of 39 genes were consistently upregulated in all four generations of adult hda-4 mutants grown under microgravity compared with artificial Earth-like gravity (1G). In contrast, in the wild type, microgravity-induced upregulation of these genes occurred a little. Among these genes, 11 contain the domain of unknown function 19 (DUF-19) and are located in a cluster on chromosome V. When compared with the 1G condition, histone H3 trimethylation at lysine 27 (H3K27me3) increased under microgravity in the DUF-19 containing genes T20D4.12 to 4.10 locus in wild-type adults. On the other hand, this increase was also observed in the hda-4 mutant, but the level was significantly reduced. The body length of wild-type adults decreased slightly but significantly when grown under microgravity. This decrease was even more pronounced with the hda-4 mutant. In ground-based experiments, one of the T20D4.11 overexpressing strains significantly reduced body length and also caused larval growth retardation and arrest. These results indicate that under microgravity, C. elegans activates histone deacetylase HDA-4 to suppress overregulation of several genes, including the DUF-19 family. In other words, the expression of certain genes, including negative regulators of growth and development, is epigenetically fine-tuned to adapt to the space microgravity.Atsushi HigashitaniToko HashizumeMai TakiuraNahoko HigashitaniMika TeranishiRika OshimaSachiko YanoKana KuriyamaAkira HigashibataNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 7, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65 Physiology QP1-981
spellingShingle	Biotechnology TP248.13-248.65 Physiology QP1-981 Atsushi Higashitani Toko Hashizume Mai Takiura Nahoko Higashitani Mika Teranishi Rika Oshima Sachiko Yano Kana Kuriyama Akira Higashibata Histone deacetylase HDA-4-mediated epigenetic regulation in space-flown C. elegans
description	Abstract Epigenetic changes during long-term spaceflight are beginning to be studied by NASA’s twin astronauts and other model organisms. Here, we evaluate the epigenetic regulation of gene expression in space-flown C. elegans by comparing wild type and histone deacetylase (hda)-4 mutants. Expression levels of 39 genes were consistently upregulated in all four generations of adult hda-4 mutants grown under microgravity compared with artificial Earth-like gravity (1G). In contrast, in the wild type, microgravity-induced upregulation of these genes occurred a little. Among these genes, 11 contain the domain of unknown function 19 (DUF-19) and are located in a cluster on chromosome V. When compared with the 1G condition, histone H3 trimethylation at lysine 27 (H3K27me3) increased under microgravity in the DUF-19 containing genes T20D4.12 to 4.10 locus in wild-type adults. On the other hand, this increase was also observed in the hda-4 mutant, but the level was significantly reduced. The body length of wild-type adults decreased slightly but significantly when grown under microgravity. This decrease was even more pronounced with the hda-4 mutant. In ground-based experiments, one of the T20D4.11 overexpressing strains significantly reduced body length and also caused larval growth retardation and arrest. These results indicate that under microgravity, C. elegans activates histone deacetylase HDA-4 to suppress overregulation of several genes, including the DUF-19 family. In other words, the expression of certain genes, including negative regulators of growth and development, is epigenetically fine-tuned to adapt to the space microgravity.
format	article
author	Atsushi Higashitani Toko Hashizume Mai Takiura Nahoko Higashitani Mika Teranishi Rika Oshima Sachiko Yano Kana Kuriyama Akira Higashibata
author_facet	Atsushi Higashitani Toko Hashizume Mai Takiura Nahoko Higashitani Mika Teranishi Rika Oshima Sachiko Yano Kana Kuriyama Akira Higashibata
author_sort	Atsushi Higashitani
title	Histone deacetylase HDA-4-mediated epigenetic regulation in space-flown C. elegans
title_short	Histone deacetylase HDA-4-mediated epigenetic regulation in space-flown C. elegans
title_full	Histone deacetylase HDA-4-mediated epigenetic regulation in space-flown C. elegans
title_fullStr	Histone deacetylase HDA-4-mediated epigenetic regulation in space-flown C. elegans
title_full_unstemmed	Histone deacetylase HDA-4-mediated epigenetic regulation in space-flown C. elegans
title_sort	histone deacetylase hda-4-mediated epigenetic regulation in space-flown c. elegans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/94dd2e3bfe9947d8accd09979545aa51
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Histone deacetylase HDA-4-mediated epigenetic regulation in space-flown C. elegans

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