Mesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen I and nanoparticulate hydroxyapatite.

The performance of biomaterials designed for bone repair depends, in part, on the ability of the material to support the adhesion and survival of mesenchymal stem cells (MSCs). In this study, a nanofibrous bone-mimicking scaffold was electrospun from a mixture of polycaprolactone (PCL), collagen I,...

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Autores principales: Matthew C Phipps, William C Clem, Shane A Catledge, Yuanyuan Xu, Kristin M Hennessy, Vinoy Thomas, Michael J Jablonsky, Shafiul Chowdhury, Andrei V Stanishevsky, Yogesh K Vohra, Susan L Bellis
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Publicado: Public Library of Science (PLoS) 2011
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spelling oai:doaj.org-article:94e3fd863d22444784ca43d8b11a0b7e2021-11-18T06:59:08ZMesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen I and nanoparticulate hydroxyapatite.1932-620310.1371/journal.pone.0016813https://doaj.org/article/94e3fd863d22444784ca43d8b11a0b7e2011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21346817/?tool=EBIhttps://doaj.org/toc/1932-6203The performance of biomaterials designed for bone repair depends, in part, on the ability of the material to support the adhesion and survival of mesenchymal stem cells (MSCs). In this study, a nanofibrous bone-mimicking scaffold was electrospun from a mixture of polycaprolactone (PCL), collagen I, and hydroxyapatite (HA) nanoparticles with a dry weight ratio of 50/30/20 respectively (PCL/col/HA). The cytocompatibility of this tri-component scaffold was compared with three other scaffold formulations: 100% PCL (PCL), 100% collagen I (col), and a bi-component scaffold containing 80% PCL/20% HA (PCL/HA). Scanning electron microscopy, fluorescent live cell imaging, and MTS assays showed that MSCs adhered to the PCL, PCL/HA and PCL/col/HA scaffolds, however more rapid cell spreading and significantly greater cell proliferation was observed for MSCs on the tri-component bone-mimetic scaffolds. In contrast, the col scaffolds did not support cell spreading or survival, possibly due to the low tensile modulus of this material. PCL/col/HA scaffolds adsorbed a substantially greater quantity of the adhesive proteins, fibronectin and vitronectin, than PCL or PCL/HA following in vitro exposure to serum, or placement into rat tibiae, which may have contributed to the favorable cell responses to the tri-component substrates. In addition, cells seeded onto PCL/col/HA scaffolds showed markedly increased levels of phosphorylated FAK, a marker of integrin activation and a signaling molecule known to be important for directing cell survival and osteoblastic differentiation. Collectively these results suggest that electrospun bone-mimetic matrices serve as promising degradable substrates for bone regenerative applications.Matthew C PhippsWilliam C ClemShane A CatledgeYuanyuan XuKristin M HennessyVinoy ThomasMichael J JablonskyShafiul ChowdhuryAndrei V StanishevskyYogesh K VohraSusan L BellisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e16813 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Matthew C Phipps
William C Clem
Shane A Catledge
Yuanyuan Xu
Kristin M Hennessy
Vinoy Thomas
Michael J Jablonsky
Shafiul Chowdhury
Andrei V Stanishevsky
Yogesh K Vohra
Susan L Bellis
Mesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen I and nanoparticulate hydroxyapatite.
description The performance of biomaterials designed for bone repair depends, in part, on the ability of the material to support the adhesion and survival of mesenchymal stem cells (MSCs). In this study, a nanofibrous bone-mimicking scaffold was electrospun from a mixture of polycaprolactone (PCL), collagen I, and hydroxyapatite (HA) nanoparticles with a dry weight ratio of 50/30/20 respectively (PCL/col/HA). The cytocompatibility of this tri-component scaffold was compared with three other scaffold formulations: 100% PCL (PCL), 100% collagen I (col), and a bi-component scaffold containing 80% PCL/20% HA (PCL/HA). Scanning electron microscopy, fluorescent live cell imaging, and MTS assays showed that MSCs adhered to the PCL, PCL/HA and PCL/col/HA scaffolds, however more rapid cell spreading and significantly greater cell proliferation was observed for MSCs on the tri-component bone-mimetic scaffolds. In contrast, the col scaffolds did not support cell spreading or survival, possibly due to the low tensile modulus of this material. PCL/col/HA scaffolds adsorbed a substantially greater quantity of the adhesive proteins, fibronectin and vitronectin, than PCL or PCL/HA following in vitro exposure to serum, or placement into rat tibiae, which may have contributed to the favorable cell responses to the tri-component substrates. In addition, cells seeded onto PCL/col/HA scaffolds showed markedly increased levels of phosphorylated FAK, a marker of integrin activation and a signaling molecule known to be important for directing cell survival and osteoblastic differentiation. Collectively these results suggest that electrospun bone-mimetic matrices serve as promising degradable substrates for bone regenerative applications.
format article
author Matthew C Phipps
William C Clem
Shane A Catledge
Yuanyuan Xu
Kristin M Hennessy
Vinoy Thomas
Michael J Jablonsky
Shafiul Chowdhury
Andrei V Stanishevsky
Yogesh K Vohra
Susan L Bellis
author_facet Matthew C Phipps
William C Clem
Shane A Catledge
Yuanyuan Xu
Kristin M Hennessy
Vinoy Thomas
Michael J Jablonsky
Shafiul Chowdhury
Andrei V Stanishevsky
Yogesh K Vohra
Susan L Bellis
author_sort Matthew C Phipps
title Mesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen I and nanoparticulate hydroxyapatite.
title_short Mesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen I and nanoparticulate hydroxyapatite.
title_full Mesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen I and nanoparticulate hydroxyapatite.
title_fullStr Mesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen I and nanoparticulate hydroxyapatite.
title_full_unstemmed Mesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen I and nanoparticulate hydroxyapatite.
title_sort mesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen i and nanoparticulate hydroxyapatite.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/94e3fd863d22444784ca43d8b11a0b7e
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