Urinary genome detection and tracking of Hantaan virus from hemorrhagic fever with renal syndrome patients using multiplex PCR-based next-generation sequencing.

<h4>Background</h4>Hantavirus infection occurs through the inhalation of aerosolized excreta, including urine, feces, and saliva of infected rodents. The presence of Hantaan virus (HTNV) RNA or infectious particles in urine specimens of patient with hemorrhagic fever with renal syndrome...

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Autores principales:	Seungchan Cho, Won-Keun Kim, Jin Sun No, Seung-Ho Lee, Jaehun Jung, Yongjin Yi, Hayne Cho Park, Geum-Young Lee, Kyungmin Park, Jeong-Ah Kim, Jongwoo Kim, Jingyeong Lee, Daesang Lee, Dong Hyun Song, Se Hun Gu, Seong Tae Jeong, Jin-Won Song
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/94f11e9311aa4843870d5ee0c87040b7
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spelling	oai:doaj.org-article:94f11e9311aa4843870d5ee0c87040b72021-12-02T20:24:01ZUrinary genome detection and tracking of Hantaan virus from hemorrhagic fever with renal syndrome patients using multiplex PCR-based next-generation sequencing.1935-27271935-273510.1371/journal.pntd.0009707https://doaj.org/article/94f11e9311aa4843870d5ee0c87040b72021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009707https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Hantavirus infection occurs through the inhalation of aerosolized excreta, including urine, feces, and saliva of infected rodents. The presence of Hantaan virus (HTNV) RNA or infectious particles in urine specimens of patient with hemorrhagic fever with renal syndrome (HFRS) remains to be investigated.<h4>Methodology/principal findings</h4>We collected four urine and serum specimens of Republic of Korea Army (ROKA) patients with HFRS. We performed multiplex PCR-based next-generation sequencing (NGS) to obtain the genome sequences of clinical HTNV in urine specimens containing ultra-low amounts of viral genomes. The epidemiological and phylogenetic analyses of HTNV demonstrated geographically homogenous clustering with those in Apodemus agrarius captured in highly endemic areas, indicating that phylogeographic tracing of HTNV genomes reveals the potential infection sites of patients with HFRS. Genetic exchange analyses showed a genetic configuration compatible with HTNV L segment exchange in nature.<h4>Conclusion/significance</h4>Our results suggest that whole or partial genome sequences of HTNV from the urine enabled to track the putative infection sites of patients with HFRS by phylogeographically linking to the zoonotic HTNV from the reservoir host captured at endemic regions. This report raises awareness among physicians for the presence of HTNV in the urine of patients with HFRS.Seungchan ChoWon-Keun KimJin Sun NoSeung-Ho LeeJaehun JungYongjin YiHayne Cho ParkGeum-Young LeeKyungmin ParkJeong-Ah KimJongwoo KimJingyeong LeeDaesang LeeDong Hyun SongSe Hun GuSeong Tae JeongJin-Won SongPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009707 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Seungchan Cho Won-Keun Kim Jin Sun No Seung-Ho Lee Jaehun Jung Yongjin Yi Hayne Cho Park Geum-Young Lee Kyungmin Park Jeong-Ah Kim Jongwoo Kim Jingyeong Lee Daesang Lee Dong Hyun Song Se Hun Gu Seong Tae Jeong Jin-Won Song Urinary genome detection and tracking of Hantaan virus from hemorrhagic fever with renal syndrome patients using multiplex PCR-based next-generation sequencing.
description	<h4>Background</h4>Hantavirus infection occurs through the inhalation of aerosolized excreta, including urine, feces, and saliva of infected rodents. The presence of Hantaan virus (HTNV) RNA or infectious particles in urine specimens of patient with hemorrhagic fever with renal syndrome (HFRS) remains to be investigated.<h4>Methodology/principal findings</h4>We collected four urine and serum specimens of Republic of Korea Army (ROKA) patients with HFRS. We performed multiplex PCR-based next-generation sequencing (NGS) to obtain the genome sequences of clinical HTNV in urine specimens containing ultra-low amounts of viral genomes. The epidemiological and phylogenetic analyses of HTNV demonstrated geographically homogenous clustering with those in Apodemus agrarius captured in highly endemic areas, indicating that phylogeographic tracing of HTNV genomes reveals the potential infection sites of patients with HFRS. Genetic exchange analyses showed a genetic configuration compatible with HTNV L segment exchange in nature.<h4>Conclusion/significance</h4>Our results suggest that whole or partial genome sequences of HTNV from the urine enabled to track the putative infection sites of patients with HFRS by phylogeographically linking to the zoonotic HTNV from the reservoir host captured at endemic regions. This report raises awareness among physicians for the presence of HTNV in the urine of patients with HFRS.
format	article
author	Seungchan Cho Won-Keun Kim Jin Sun No Seung-Ho Lee Jaehun Jung Yongjin Yi Hayne Cho Park Geum-Young Lee Kyungmin Park Jeong-Ah Kim Jongwoo Kim Jingyeong Lee Daesang Lee Dong Hyun Song Se Hun Gu Seong Tae Jeong Jin-Won Song
author_facet	Seungchan Cho Won-Keun Kim Jin Sun No Seung-Ho Lee Jaehun Jung Yongjin Yi Hayne Cho Park Geum-Young Lee Kyungmin Park Jeong-Ah Kim Jongwoo Kim Jingyeong Lee Daesang Lee Dong Hyun Song Se Hun Gu Seong Tae Jeong Jin-Won Song
author_sort	Seungchan Cho
title	Urinary genome detection and tracking of Hantaan virus from hemorrhagic fever with renal syndrome patients using multiplex PCR-based next-generation sequencing.
title_short	Urinary genome detection and tracking of Hantaan virus from hemorrhagic fever with renal syndrome patients using multiplex PCR-based next-generation sequencing.
title_full	Urinary genome detection and tracking of Hantaan virus from hemorrhagic fever with renal syndrome patients using multiplex PCR-based next-generation sequencing.
title_fullStr	Urinary genome detection and tracking of Hantaan virus from hemorrhagic fever with renal syndrome patients using multiplex PCR-based next-generation sequencing.
title_full_unstemmed	Urinary genome detection and tracking of Hantaan virus from hemorrhagic fever with renal syndrome patients using multiplex PCR-based next-generation sequencing.
title_sort	urinary genome detection and tracking of hantaan virus from hemorrhagic fever with renal syndrome patients using multiplex pcr-based next-generation sequencing.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/94f11e9311aa4843870d5ee0c87040b7
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Urinary genome detection and tracking of Hantaan virus from hemorrhagic fever with renal syndrome patients using multiplex PCR-based next-generation sequencing.

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