A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods

This study aims to determine the prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii by three methods of diagnosis (microscopic examination, cultivation and PCR) that were compared to obtain an accurate diagnosis of Entamoeba spp. during amoebiasis. Total (n=150) stool s...

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Autores principales:	Athraa Abd-ulameer Al-Hilfy, Maha Khalil Al-Malak, Muslim Abdulrahman Al-Tomah
Formato:	article
Lenguaje:	AR EN
Publicado:	College of Science for Women, University of Baghdad 2021
Materias:	Entamoeba spp, Cultivation, PCR. Science Q
Acceso en línea:	https://doaj.org/article/94f2555eac964426a9f8cee7ca3bea76
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spelling	oai:doaj.org-article:94f2555eac964426a9f8cee7ca3bea762021-12-04T16:13:08ZA Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods10.21123/bsj.2021.18.4.11632078-86652411-7986https://doaj.org/article/94f2555eac964426a9f8cee7ca3bea762021-12-01T00:00:00Zhttps://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4014https://doaj.org/toc/2078-8665https://doaj.org/toc/2411-7986 This study aims to determine the prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii by three methods of diagnosis (microscopic examination, cultivation and PCR) that were compared to obtain an accurate diagnosis of Entamoeba spp. during amoebiasis. Total (n=150) stool samples related to patients were (n = 100) and healthy controls (n= 50). Clinically diagnosed stool samples (n=100) were collected from patients attending the consultant clinics of different hospitals in Basrah during the period from January 2018 to January 2019. The results showed that 60% of collected samples were positive in a direct microscopic examination. All samples were cultivated on different media; the Brain heart infusion agar showed high efficiency and was the most suitable in cultivating the parasite. Data and results of molecular study were indicated by DNA extraction from stool samples and used in PCR technique with specific primers. This study identifies different infection percentage for the three species. The highest infection in Basrah patients was Entamoeba moshkovskii 15% followed by Entamoeba dispar 10% and Entamoeba histolytica, which was 5%. Athraa Abd-ulameer Al-HilfyMaha Khalil Al-MalakMuslim Abdulrahman Al-TomahCollege of Science for Women, University of BaghdadarticleEntamoeba spp, Cultivation, PCR.ScienceQARENBaghdad Science Journal, Vol 18, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	AR EN
topic	Entamoeba spp, Cultivation, PCR. Science Q
spellingShingle	Entamoeba spp, Cultivation, PCR. Science Q Athraa Abd-ulameer Al-Hilfy Maha Khalil Al-Malak Muslim Abdulrahman Al-Tomah A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods
description	This study aims to determine the prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii by three methods of diagnosis (microscopic examination, cultivation and PCR) that were compared to obtain an accurate diagnosis of Entamoeba spp. during amoebiasis. Total (n=150) stool samples related to patients were (n = 100) and healthy controls (n= 50). Clinically diagnosed stool samples (n=100) were collected from patients attending the consultant clinics of different hospitals in Basrah during the period from January 2018 to January 2019. The results showed that 60% of collected samples were positive in a direct microscopic examination. All samples were cultivated on different media; the Brain heart infusion agar showed high efficiency and was the most suitable in cultivating the parasite. Data and results of molecular study were indicated by DNA extraction from stool samples and used in PCR technique with specific primers. This study identifies different infection percentage for the three species. The highest infection in Basrah patients was Entamoeba moshkovskii 15% followed by Entamoeba dispar 10% and Entamoeba histolytica, which was 5%.
format	article
author	Athraa Abd-ulameer Al-Hilfy Maha Khalil Al-Malak Muslim Abdulrahman Al-Tomah
author_facet	Athraa Abd-ulameer Al-Hilfy Maha Khalil Al-Malak Muslim Abdulrahman Al-Tomah
author_sort	Athraa Abd-ulameer Al-Hilfy
title	A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods
title_short	A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods
title_full	A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods
title_fullStr	A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods
title_full_unstemmed	A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods
title_sort	prevalence study of entamoeba spp. in basrah province using different detection methods
publisher	College of Science for Women, University of Baghdad
publishDate	2021
url	https://doaj.org/article/94f2555eac964426a9f8cee7ca3bea76
work_keys_str_mv	AT athraaabdulameeralhilfy aprevalencestudyofentamoebasppinbasrahprovinceusingdifferentdetectionmethods AT mahakhalilalmalak aprevalencestudyofentamoebasppinbasrahprovinceusingdifferentdetectionmethods AT muslimabdulrahmanaltomah aprevalencestudyofentamoebasppinbasrahprovinceusingdifferentdetectionmethods AT athraaabdulameeralhilfy prevalencestudyofentamoebasppinbasrahprovinceusingdifferentdetectionmethods AT mahakhalilalmalak prevalencestudyofentamoebasppinbasrahprovinceusingdifferentdetectionmethods AT muslimabdulrahmanaltomah prevalencestudyofentamoebasppinbasrahprovinceusingdifferentdetectionmethods
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A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods

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