Optimal single sampling time-point for monitoring of praziquantel exposure in children

Abstract Praziquantel pharmacokinetics studies in schistosomiasis infected children are scarce partly due to the challenges/complexity of intensive blood sampling in the target population. This study was aimed to investigate the optimal single sampling time-point for monitoring praziquantel exposure...

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Autores principales:	Rajabu Hussein Mnkugwe, Eliford Ngaimisi Kitabi, Safari Kinung’hi, Appolinary A. R. Kamuhabwa, Omary Mashiku Minzi, Eleni Aklillu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/94f4d46daa4948c4ba9c381e4f310157
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spelling	oai:doaj.org-article:94f4d46daa4948c4ba9c381e4f3101572021-12-02T17:19:15ZOptimal single sampling time-point for monitoring of praziquantel exposure in children10.1038/s41598-021-97409-x2045-2322https://doaj.org/article/94f4d46daa4948c4ba9c381e4f3101572021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97409-xhttps://doaj.org/toc/2045-2322Abstract Praziquantel pharmacokinetics studies in schistosomiasis infected children are scarce partly due to the challenges/complexity of intensive blood sampling in the target population. This study was aimed to investigate the optimal single sampling time-point for monitoring praziquantel exposure. This was intensive pharmacokinetic study conducted among 32 Schistosoma mansoni infected children treated with an oral standard single-dose 40 mg/kg praziquantel. Plasma samples were collected at 0, 1, 2, 4, 6 and 8 h post-praziquantel administration. Quantification of praziquantel and its enantiomers (R- and S-praziquantel) concentrations was done by Liquid chromatography-tandem mass spectrometer (LC–MS/MS). The correlation between area under the plasma concentration–time curve from 0 to 8 h (AUC8) and plasma concentrations at each specific sampling time-point was determined by Pearson’s correlation coefficient (r2). The median age (range) of the study population was 12.5 years (10–17). The study participants were 17 males and 15 females. Both total praziquantel and its enantiomers (R- and S-praziquantel) displayed a wide inter-individual pharmacokinetic variability. Regression analysis indicated that, plasma concentrations collected at 4 h post-dose had a significantly highest correlation with the AUC8 for both total praziquantel (r2 = 0.81, p < 0.001) and S-praziquantel (r2 = 0.84, p < 0.001) than any other sampling time-point; while for R-praziquantel, plasma concentrations collected at 6 h sampling time-point had a significantly highest correlation with the AUC8 (r2 = 0.79, p < 0.001) than any other sampling time-point. Four hours sampling time-point post-praziquantel administration is ideal optimal single sampling time-point for therapeutic monitoring of total praziquantel exposure while 6 h sampling time-point is suitable for monitoring of a pharmacologically active R-praziquantel enantiomer.Rajabu Hussein MnkugweEliford Ngaimisi KitabiSafari Kinung’hiAppolinary A. R. KamuhabwaOmary Mashiku MinziEleni AklilluNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rajabu Hussein Mnkugwe Eliford Ngaimisi Kitabi Safari Kinung’hi Appolinary A. R. Kamuhabwa Omary Mashiku Minzi Eleni Aklillu Optimal single sampling time-point for monitoring of praziquantel exposure in children
description	Abstract Praziquantel pharmacokinetics studies in schistosomiasis infected children are scarce partly due to the challenges/complexity of intensive blood sampling in the target population. This study was aimed to investigate the optimal single sampling time-point for monitoring praziquantel exposure. This was intensive pharmacokinetic study conducted among 32 Schistosoma mansoni infected children treated with an oral standard single-dose 40 mg/kg praziquantel. Plasma samples were collected at 0, 1, 2, 4, 6 and 8 h post-praziquantel administration. Quantification of praziquantel and its enantiomers (R- and S-praziquantel) concentrations was done by Liquid chromatography-tandem mass spectrometer (LC–MS/MS). The correlation between area under the plasma concentration–time curve from 0 to 8 h (AUC8) and plasma concentrations at each specific sampling time-point was determined by Pearson’s correlation coefficient (r2). The median age (range) of the study population was 12.5 years (10–17). The study participants were 17 males and 15 females. Both total praziquantel and its enantiomers (R- and S-praziquantel) displayed a wide inter-individual pharmacokinetic variability. Regression analysis indicated that, plasma concentrations collected at 4 h post-dose had a significantly highest correlation with the AUC8 for both total praziquantel (r2 = 0.81, p < 0.001) and S-praziquantel (r2 = 0.84, p < 0.001) than any other sampling time-point; while for R-praziquantel, plasma concentrations collected at 6 h sampling time-point had a significantly highest correlation with the AUC8 (r2 = 0.79, p < 0.001) than any other sampling time-point. Four hours sampling time-point post-praziquantel administration is ideal optimal single sampling time-point for therapeutic monitoring of total praziquantel exposure while 6 h sampling time-point is suitable for monitoring of a pharmacologically active R-praziquantel enantiomer.
format	article
author	Rajabu Hussein Mnkugwe Eliford Ngaimisi Kitabi Safari Kinung’hi Appolinary A. R. Kamuhabwa Omary Mashiku Minzi Eleni Aklillu
author_facet	Rajabu Hussein Mnkugwe Eliford Ngaimisi Kitabi Safari Kinung’hi Appolinary A. R. Kamuhabwa Omary Mashiku Minzi Eleni Aklillu
author_sort	Rajabu Hussein Mnkugwe
title	Optimal single sampling time-point for monitoring of praziquantel exposure in children
title_short	Optimal single sampling time-point for monitoring of praziquantel exposure in children
title_full	Optimal single sampling time-point for monitoring of praziquantel exposure in children
title_fullStr	Optimal single sampling time-point for monitoring of praziquantel exposure in children
title_full_unstemmed	Optimal single sampling time-point for monitoring of praziquantel exposure in children
title_sort	optimal single sampling time-point for monitoring of praziquantel exposure in children
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/94f4d46daa4948c4ba9c381e4f310157
work_keys_str_mv	AT rajabuhusseinmnkugwe optimalsinglesamplingtimepointformonitoringofpraziquantelexposureinchildren AT elifordngaimisikitabi optimalsinglesamplingtimepointformonitoringofpraziquantelexposureinchildren AT safarikinunghi optimalsinglesamplingtimepointformonitoringofpraziquantelexposureinchildren AT appolinaryarkamuhabwa optimalsinglesamplingtimepointformonitoringofpraziquantelexposureinchildren AT omarymashikuminzi optimalsinglesamplingtimepointformonitoringofpraziquantelexposureinchildren AT eleniaklillu optimalsinglesamplingtimepointformonitoringofpraziquantelexposureinchildren
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Optimal single sampling time-point for monitoring of praziquantel exposure in children

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