Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound
Feng-Yi Yang1,*, Hsin-Ell Wang1,*, Guan-Liang Lin1, Hui-Hsien Lin1, Tai-Tong Wong21Department of Biomedical Imaging and Radiological Sciences, School of Biomedical Science and Engineering, National Yang-Ming University, 2Department of Neurosurgery, Neurological Institute, Taipei Veterans General Hos...
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Dove Medical Press
2012
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oai:doaj.org-article:94f8646141cf4a04ba6cfd267f2e6a1a2021-12-02T01:43:42ZEvaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound1176-91141178-2013https://doaj.org/article/94f8646141cf4a04ba6cfd267f2e6a1a2012-02-01T00:00:00Zhttp://www.dovepress.com/evaluation-of-the-increase-in-permeability-of-the-bloodndashbrain-barr-a9248https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Feng-Yi Yang1,*, Hsin-Ell Wang1,*, Guan-Liang Lin1, Hui-Hsien Lin1, Tai-Tong Wong21Department of Biomedical Imaging and Radiological Sciences, School of Biomedical Science and Engineering, National Yang-Ming University, 2Department of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan *These authors contributed equally to this workPurpose: The purpose of this study was to evaluate the permeability of the blood–brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio.Materials and methods: F98 glioma-bearing Fischer 344 rats were injected intravenously with Evans blue with or without blood–tumor barrier disruption induced by transcranial pulsed high-intensity focused ultrasound. Sonication was applied at a frequency of 1 MHz with a 5% duty cycle and a repetition frequency of 1 Hz. The permeability of the blood–brain barrier was assessed by the extravasation of Evans blue. Contrast-enhanced magnetic resonance images were used to monitor the gadolinium deposition path associated with transcranial pulsed high-intensity focused ultrasound, and the influencing size and location was also investigated. In addition, whole brain histological analysis was performed. The results were compared by two-tailed unpaired t-test.Results: The accumulation of Evans blue in brains and the tumor:ipsilateral brain permeability ratio of Evans blue were significantly increased after pulsed high-intensity focused ultrasound exposure. Evans blue injection followed by sonication showed an increase in the tumor:ipsilateral brain ratio of the target tumors (9.14:1) of about 2.23-fold compared with the control tumors (x4.09) on day 6 after tumor implantation. Magnetic resonance images showed that pulsed high-intensity focused ultrasound locally enhances the permeability of the blood–tumor barrier in the glioma-bearing rats.Conclusion: This method could allow enhanced synergistic effects with respect to other brain tumor treatment regimens.Keywords: focused ultrasound, blood–brain barrier, permeability, brain tumor, gliomaWong TTLin HHLin GLWang HEYang FYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 723-730 (2012) |
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Medicine (General) R5-920 |
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Medicine (General) R5-920 Wong TT Lin HH Lin GL Wang HE Yang FY Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
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Feng-Yi Yang1,*, Hsin-Ell Wang1,*, Guan-Liang Lin1, Hui-Hsien Lin1, Tai-Tong Wong21Department of Biomedical Imaging and Radiological Sciences, School of Biomedical Science and Engineering, National Yang-Ming University, 2Department of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan *These authors contributed equally to this workPurpose: The purpose of this study was to evaluate the permeability of the blood–brain barrier after sonication by pulsed high-intensity focused ultrasound and to determine if such an approach increases the tumor:ipsilateral brain permeability ratio.Materials and methods: F98 glioma-bearing Fischer 344 rats were injected intravenously with Evans blue with or without blood–tumor barrier disruption induced by transcranial pulsed high-intensity focused ultrasound. Sonication was applied at a frequency of 1 MHz with a 5% duty cycle and a repetition frequency of 1 Hz. The permeability of the blood–brain barrier was assessed by the extravasation of Evans blue. Contrast-enhanced magnetic resonance images were used to monitor the gadolinium deposition path associated with transcranial pulsed high-intensity focused ultrasound, and the influencing size and location was also investigated. In addition, whole brain histological analysis was performed. The results were compared by two-tailed unpaired t-test.Results: The accumulation of Evans blue in brains and the tumor:ipsilateral brain permeability ratio of Evans blue were significantly increased after pulsed high-intensity focused ultrasound exposure. Evans blue injection followed by sonication showed an increase in the tumor:ipsilateral brain ratio of the target tumors (9.14:1) of about 2.23-fold compared with the control tumors (x4.09) on day 6 after tumor implantation. Magnetic resonance images showed that pulsed high-intensity focused ultrasound locally enhances the permeability of the blood–tumor barrier in the glioma-bearing rats.Conclusion: This method could allow enhanced synergistic effects with respect to other brain tumor treatment regimens.Keywords: focused ultrasound, blood–brain barrier, permeability, brain tumor, glioma |
| format |
article |
| author |
Wong TT Lin HH Lin GL Wang HE Yang FY |
| author_facet |
Wong TT Lin HH Lin GL Wang HE Yang FY |
| author_sort |
Wong TT |
| title |
Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
| title_short |
Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
| title_full |
Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
| title_fullStr |
Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
| title_full_unstemmed |
Evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
| title_sort |
evaluation of the increase in permeability of the blood–brain barrier during tumor progression after pulsed focused ultrasound |
| publisher |
Dove Medical Press |
| publishDate |
2012 |
| url |
https://doaj.org/article/94f8646141cf4a04ba6cfd267f2e6a1a |
| work_keys_str_mv |
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