$Anti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS$
Código QR

Anti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS

Ivy leaves (Hedera helix) is a traditional plant used for common cold, cough, and bronchial disorders and can be used for rheumatoid arthritis (RA) as an attempt in alternative medicine. RA is a chronic autoimmune disease characterized by its increasing frequency and adverse consequences. There is a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aya A. Shokry, Riham A. El-Shiekh, Gehan Kamel, Alaa F. Bakr, Dina Sabry, Amer Ramadan
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Flavonoids Saponins Inflammatory cytokines Oxidative stress Adjuvant- induced arthritis Rheumatoid biomarkers Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/94fbf551d618465899d2fa2f253feb21
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:94fbf551d618465899d2fa2f253feb21
record_format	dspace
spelling	oai:doaj.org-article:94fbf551d618465899d2fa2f253feb212021-11-28T04:28:18ZAnti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS0753-332210.1016/j.biopha.2021.112456https://doaj.org/article/94fbf551d618465899d2fa2f253feb212022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221012427https://doaj.org/toc/0753-3322Ivy leaves (Hedera helix) is a traditional plant used for common cold, cough, and bronchial disorders and can be used for rheumatoid arthritis (RA) as an attempt in alternative medicine. RA is a chronic autoimmune disease characterized by its increasing frequency and adverse consequences. There is an urgent need for a long-term therapy that has favorable biological effects and is less expensive than the already authorized synthetic medicines. This study aimed to determine the anti-arthritic potentials of Hedera helix with determination of the bioactive fraction and discovery of its second-generation metabolites by means of LC/MS. The total ivy ethanolic extract (TIE-E), saponins fraction (Sap-F) and flavonoids fraction (Flav-F) were investigated for their in-vitro anti-arthritic effects and in-vivo by Adjuvant-induced arthritis (AIA) using Complete Freund’s Adjuvant (0.1 mL, CFA) intradermal relative to the usual dose of ibuprofen (5 mg/kg). We examined the physical alterations, rheumatoid biomarkers, cytokines that cause and inhibit inflammation, markers of oxidative stress, hyaluronidase and β-glucuronidase enzyme activity. Each paw's histopathology was also evaluated. The chemical profiles of TIE-E were studied using LC/MS in both positive and negative ionization modes. TIE-E (200 mg/kg) and Flav-F (100 mg/kg) significantly (P < 0.05) lowered the edema of the paws, serum immunological indicators, inflammatory cytokines, degenerative enzymes, and indicators of reactive oxygen species with increasing in the anti-inflammatory cytokines. Our findings suggest that extracts of ivy leaves might be used effectively to treat rheumatoid arthritis, where its flavonoid content is responsible for that, and it is able to repress biochemical, oxidative, and pathological changes associated with (AIA) Adjuvant-induced arthritis.Aya A. ShokryRiham A. El-ShiekhGehan KamelAlaa F. BakrDina SabryAmer RamadanElsevierarticleFlavonoidsSaponinsInflammatory cytokinesOxidative stressAdjuvant- induced arthritisRheumatoid biomarkersTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112456- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Flavonoids Saponins Inflammatory cytokines Oxidative stress Adjuvant- induced arthritis Rheumatoid biomarkers Therapeutics. Pharmacology RM1-950
spellingShingle	Flavonoids Saponins Inflammatory cytokines Oxidative stress Adjuvant- induced arthritis Rheumatoid biomarkers Therapeutics. Pharmacology RM1-950 Aya A. Shokry Riham A. El-Shiekh Gehan Kamel Alaa F. Bakr Dina Sabry Amer Ramadan Anti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS
description	Ivy leaves (Hedera helix) is a traditional plant used for common cold, cough, and bronchial disorders and can be used for rheumatoid arthritis (RA) as an attempt in alternative medicine. RA is a chronic autoimmune disease characterized by its increasing frequency and adverse consequences. There is an urgent need for a long-term therapy that has favorable biological effects and is less expensive than the already authorized synthetic medicines. This study aimed to determine the anti-arthritic potentials of Hedera helix with determination of the bioactive fraction and discovery of its second-generation metabolites by means of LC/MS. The total ivy ethanolic extract (TIE-E), saponins fraction (Sap-F) and flavonoids fraction (Flav-F) were investigated for their in-vitro anti-arthritic effects and in-vivo by Adjuvant-induced arthritis (AIA) using Complete Freund’s Adjuvant (0.1 mL, CFA) intradermal relative to the usual dose of ibuprofen (5 mg/kg). We examined the physical alterations, rheumatoid biomarkers, cytokines that cause and inhibit inflammation, markers of oxidative stress, hyaluronidase and β-glucuronidase enzyme activity. Each paw's histopathology was also evaluated. The chemical profiles of TIE-E were studied using LC/MS in both positive and negative ionization modes. TIE-E (200 mg/kg) and Flav-F (100 mg/kg) significantly (P < 0.05) lowered the edema of the paws, serum immunological indicators, inflammatory cytokines, degenerative enzymes, and indicators of reactive oxygen species with increasing in the anti-inflammatory cytokines. Our findings suggest that extracts of ivy leaves might be used effectively to treat rheumatoid arthritis, where its flavonoid content is responsible for that, and it is able to repress biochemical, oxidative, and pathological changes associated with (AIA) Adjuvant-induced arthritis.
format	article
author	Aya A. Shokry Riham A. El-Shiekh Gehan Kamel Alaa F. Bakr Dina Sabry Amer Ramadan
author_facet	Aya A. Shokry Riham A. El-Shiekh Gehan Kamel Alaa F. Bakr Dina Sabry Amer Ramadan
author_sort	Aya A. Shokry
title	Anti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS
title_short	Anti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS
title_full	Anti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS
title_fullStr	Anti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS
title_full_unstemmed	Anti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS
title_sort	anti-arthritic activity of the flavonoids fraction of ivy leaves (hedera helix l.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using lc/ms
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/94fbf551d618465899d2fa2f253feb21
work_keys_str_mv	AT ayaashokry antiarthriticactivityoftheflavonoidsfractionofivyleaveshederahelixlstandardizedextractinadjuvantinducedarthritismodelinratsinrelationtoitsmetaboliteprofileusinglcms AT rihamaelshiekh antiarthriticactivityoftheflavonoidsfractionofivyleaveshederahelixlstandardizedextractinadjuvantinducedarthritismodelinratsinrelationtoitsmetaboliteprofileusinglcms AT gehankamel antiarthriticactivityoftheflavonoidsfractionofivyleaveshederahelixlstandardizedextractinadjuvantinducedarthritismodelinratsinrelationtoitsmetaboliteprofileusinglcms AT alaafbakr antiarthriticactivityoftheflavonoidsfractionofivyleaveshederahelixlstandardizedextractinadjuvantinducedarthritismodelinratsinrelationtoitsmetaboliteprofileusinglcms AT dinasabry antiarthriticactivityoftheflavonoidsfractionofivyleaveshederahelixlstandardizedextractinadjuvantinducedarthritismodelinratsinrelationtoitsmetaboliteprofileusinglcms AT amerramadan antiarthriticactivityoftheflavonoidsfractionofivyleaveshederahelixlstandardizedextractinadjuvantinducedarthritismodelinratsinrelationtoitsmetaboliteprofileusinglcms
_version_	1718408394475307008

Anti-arthritic activity of the flavonoids fraction of ivy leaves (Hedera helix L.) standardized extract in adjuvant induced arthritis model in rats in relation to its metabolite profile using LC/MS

Ejemplares similares