Burkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine

Abstract Burkholderia pseudomallei is a Gram-negative, facultative intracellular bacillus that causes the disease melioidosis. B. pseudomallei expresses a number of proteins that contribute to its intracellular survival in the mammalian host. We previously demonstrated that immunization with OMVs de...

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Autores principales:	Sarah M. Baker, Erik W. Settles, Christopher Davitt, Patrick Gellings, Nicole Kikendall, Joseph Hoffmann, Yihui Wang, Jacob Bitoun, Kasi-Russell Lodrigue, Jason W. Sahl, Paul Keim, Chad Roy, James McLachlan, Lisa A. Morici
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/94ff28ca65e5414195014839c12c40a0
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spelling	oai:doaj.org-article:94ff28ca65e5414195014839c12c40a02021-12-02T14:16:48ZBurkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine10.1038/s41541-021-00281-z2059-0105https://doaj.org/article/94ff28ca65e5414195014839c12c40a02021-01-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00281-zhttps://doaj.org/toc/2059-0105Abstract Burkholderia pseudomallei is a Gram-negative, facultative intracellular bacillus that causes the disease melioidosis. B. pseudomallei expresses a number of proteins that contribute to its intracellular survival in the mammalian host. We previously demonstrated that immunization with OMVs derived from B. pseudomallei grown in nutrient-rich media protects mice against lethal disease. Here, we evaluated if OMVs derived from B. pseudomallei grown under macrophage-mimicking growth conditions could be enriched with intracellular-stage proteins in order to improve the vaccine. We show that OMVs produced in this manner (M9 OMVs) contain proteins associated with intracellular survival yet are non-toxic to living cells. Immunization of mice provides significant protection against pulmonary infection similar to that achieved with a live attenuated vaccine and is associated with increased IgG, CD4+, and CD8+ T cells. OMVs possess inherent adjuvanticity and drive DC activation and maturation. These results indicate that M9 OMVs constitute a new promising vaccine against melioidosis.Sarah M. BakerErik W. SettlesChristopher DavittPatrick GellingsNicole KikendallJoseph HoffmannYihui WangJacob BitounKasi-Russell LodrigueJason W. SahlPaul KeimChad RoyJames McLachlanLisa A. MoriciNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Sarah M. Baker Erik W. Settles Christopher Davitt Patrick Gellings Nicole Kikendall Joseph Hoffmann Yihui Wang Jacob Bitoun Kasi-Russell Lodrigue Jason W. Sahl Paul Keim Chad Roy James McLachlan Lisa A. Morici Burkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine
description	Abstract Burkholderia pseudomallei is a Gram-negative, facultative intracellular bacillus that causes the disease melioidosis. B. pseudomallei expresses a number of proteins that contribute to its intracellular survival in the mammalian host. We previously demonstrated that immunization with OMVs derived from B. pseudomallei grown in nutrient-rich media protects mice against lethal disease. Here, we evaluated if OMVs derived from B. pseudomallei grown under macrophage-mimicking growth conditions could be enriched with intracellular-stage proteins in order to improve the vaccine. We show that OMVs produced in this manner (M9 OMVs) contain proteins associated with intracellular survival yet are non-toxic to living cells. Immunization of mice provides significant protection against pulmonary infection similar to that achieved with a live attenuated vaccine and is associated with increased IgG, CD4+, and CD8+ T cells. OMVs possess inherent adjuvanticity and drive DC activation and maturation. These results indicate that M9 OMVs constitute a new promising vaccine against melioidosis.
format	article
author	Sarah M. Baker Erik W. Settles Christopher Davitt Patrick Gellings Nicole Kikendall Joseph Hoffmann Yihui Wang Jacob Bitoun Kasi-Russell Lodrigue Jason W. Sahl Paul Keim Chad Roy James McLachlan Lisa A. Morici
author_facet	Sarah M. Baker Erik W. Settles Christopher Davitt Patrick Gellings Nicole Kikendall Joseph Hoffmann Yihui Wang Jacob Bitoun Kasi-Russell Lodrigue Jason W. Sahl Paul Keim Chad Roy James McLachlan Lisa A. Morici
author_sort	Sarah M. Baker
title	Burkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine
title_short	Burkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine
title_full	Burkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine
title_fullStr	Burkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine
title_full_unstemmed	Burkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine
title_sort	burkholderia pseudomallei omvs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/94ff28ca65e5414195014839c12c40a0
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Burkholderia pseudomallei OMVs derived from infection mimicking conditions elicit similar protection to a live-attenuated vaccine

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