Preparation and Evaluation of Recombinant Human Erythropoietin Loaded Tween 80-Albumin Nanoparticle for Traumatic Brain Injury Treatment

Yuanfeng Xue,1,* Junhong Ding,1,* Yulong Liu,2 Yuchun Pan,1 Penglai Zhao,3 Zhiwen Ren,1 Jian Xu,1 Liangliang Ye,1 Ying Xu2 1Department of Neurosurgery, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch Southeast University, Nanjing 211200, People’s Republic of Chin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xue Y, Ding J, Liu Y, Pan Y, Zhao P, Ren Z, Xu J, Ye L, Xu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	traumatic brain injury rh-erythropoietin tween 80 modified albumin nanoparticle electrostatic spray technology Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9503361fa519407f96537fcfc37800ae
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9503361fa519407f96537fcfc37800ae
record_format	dspace
spelling	oai:doaj.org-article:9503361fa519407f96537fcfc37800ae2021-12-02T10:21:50ZPreparation and Evaluation of Recombinant Human Erythropoietin Loaded Tween 80-Albumin Nanoparticle for Traumatic Brain Injury Treatment1178-2013https://doaj.org/article/9503361fa519407f96537fcfc37800ae2020-10-01T00:00:00Zhttps://www.dovepress.com/preparation-and-evaluation-of-recombinant-human-erythropoietin-loaded--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yuanfeng Xue,1,* Junhong Ding,1,* Yulong Liu,2 Yuchun Pan,1 Penglai Zhao,3 Zhiwen Ren,1 Jian Xu,1 Liangliang Ye,1 Ying Xu2 1Department of Neurosurgery, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch Southeast University, Nanjing 211200, People’s Republic of China; 2College of Pharmacy, Jiangsu University, Zhenjiang 212013, People’s Republic of China; 3Department of Neurosurgery, Brain Hospital Affiliated to Nanjing Medical University, Nanjing 210029, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yuanfeng Xue; Ying Xu Tel +8613951706820; +8651185038451Email 672455219@qq.com; ingyx@sina.com.cnObjective: Traumatic brain injury (TBI) is a serious health problem with few available treatment options. Rh-erythropoietin (rh-EPO) is a potential therapeutic drug for TBI, but it cannot cross the blood–brain barrier (BBB) directly. In this regard, a novel strategy to deliver rh-EPO for enhanced TBI treatment is via the development of Tween 80 modified albumin nanoparticles using electrostatic spray technology.Methods: The rh-EPO loaded Tween 80 modified albumin nanoparticles (rh-EPO-Tw-ABNPs) were prepared by electrostatic spray technology, while the process parameters were optimized via a single factor design. Investigation of physicochemical properties, bioactivity and stability of rh-EPO-Tw-ABNPs was carried out. The in vitro release and biocompatibility with nerve cells were also analyzed. The in vivo brain targeting efficiency, brain edema relieving effect and the expression of aquaporin 4 (AQP4) and glial fibrillary acidic protein (GFAP) in the brain were evaluated in TBI model rats.Results: The particle size of optimal rh-EPO-Tw-ABNPs was about 438 ± 45 nm, with a zeta potential of − 25.42 ± 0.8 mv. The average drug loading ratio of rh-EPO-Tw-ABNPs was 21.3± 3.7 IU/mg with a relative bioactivity of 91.6 ± 4.1%. The in vitro release of rh-EPO from the nanoparticles was rather slow, while neither the blank Tw-ABNPs nor rh-EPO-Tw-ABNPs exhibited toxicity on the microglia cells. Furthermore, in vivo experiments indicated that the rh-EPO-Tw-ABNPs could enhance the distribution of EPO in the brain and relieve brain edema more effectively. Moreover, compared with an rh-EPO injection, the rh-EPO-Tw-ABNPs could increase the AQP4 level but reduced GFAP expression in the brain with more efficiency.Conclusion: The rh-EPO-Tw-ABNPs could enhance the transport of rh-EPO into the brain with superior therapeutic effect for TBI.Keywords: traumatic brain injury, rh-erythropoietin, Tween 80 modified albumin nanoparticle, electrostatic spray technologyXue YDing JLiu YPan YZhao PRen ZXu JYe LXu YDove Medical Pressarticletraumatic brain injuryrh-erythropoietintween 80 modified albumin nanoparticleelectrostatic spray technologyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 8495-8506 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	traumatic brain injury rh-erythropoietin tween 80 modified albumin nanoparticle electrostatic spray technology Medicine (General) R5-920
spellingShingle	traumatic brain injury rh-erythropoietin tween 80 modified albumin nanoparticle electrostatic spray technology Medicine (General) R5-920 Xue Y Ding J Liu Y Pan Y Zhao P Ren Z Xu J Ye L Xu Y Preparation and Evaluation of Recombinant Human Erythropoietin Loaded Tween 80-Albumin Nanoparticle for Traumatic Brain Injury Treatment
description	Yuanfeng Xue,1,* Junhong Ding,1,* Yulong Liu,2 Yuchun Pan,1 Penglai Zhao,3 Zhiwen Ren,1 Jian Xu,1 Liangliang Ye,1 Ying Xu2 1Department of Neurosurgery, Nanjing Lishui People’s Hospital, Zhongda Hospital Lishui Branch Southeast University, Nanjing 211200, People’s Republic of China; 2College of Pharmacy, Jiangsu University, Zhenjiang 212013, People’s Republic of China; 3Department of Neurosurgery, Brain Hospital Affiliated to Nanjing Medical University, Nanjing 210029, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yuanfeng Xue; Ying Xu Tel +8613951706820; +8651185038451Email 672455219@qq.com; ingyx@sina.com.cnObjective: Traumatic brain injury (TBI) is a serious health problem with few available treatment options. Rh-erythropoietin (rh-EPO) is a potential therapeutic drug for TBI, but it cannot cross the blood–brain barrier (BBB) directly. In this regard, a novel strategy to deliver rh-EPO for enhanced TBI treatment is via the development of Tween 80 modified albumin nanoparticles using electrostatic spray technology.Methods: The rh-EPO loaded Tween 80 modified albumin nanoparticles (rh-EPO-Tw-ABNPs) were prepared by electrostatic spray technology, while the process parameters were optimized via a single factor design. Investigation of physicochemical properties, bioactivity and stability of rh-EPO-Tw-ABNPs was carried out. The in vitro release and biocompatibility with nerve cells were also analyzed. The in vivo brain targeting efficiency, brain edema relieving effect and the expression of aquaporin 4 (AQP4) and glial fibrillary acidic protein (GFAP) in the brain were evaluated in TBI model rats.Results: The particle size of optimal rh-EPO-Tw-ABNPs was about 438 ± 45 nm, with a zeta potential of − 25.42 ± 0.8 mv. The average drug loading ratio of rh-EPO-Tw-ABNPs was 21.3± 3.7 IU/mg with a relative bioactivity of 91.6 ± 4.1%. The in vitro release of rh-EPO from the nanoparticles was rather slow, while neither the blank Tw-ABNPs nor rh-EPO-Tw-ABNPs exhibited toxicity on the microglia cells. Furthermore, in vivo experiments indicated that the rh-EPO-Tw-ABNPs could enhance the distribution of EPO in the brain and relieve brain edema more effectively. Moreover, compared with an rh-EPO injection, the rh-EPO-Tw-ABNPs could increase the AQP4 level but reduced GFAP expression in the brain with more efficiency.Conclusion: The rh-EPO-Tw-ABNPs could enhance the transport of rh-EPO into the brain with superior therapeutic effect for TBI.Keywords: traumatic brain injury, rh-erythropoietin, Tween 80 modified albumin nanoparticle, electrostatic spray technology
format	article
author	Xue Y Ding J Liu Y Pan Y Zhao P Ren Z Xu J Ye L Xu Y
author_facet	Xue Y Ding J Liu Y Pan Y Zhao P Ren Z Xu J Ye L Xu Y
author_sort	Xue Y
title	Preparation and Evaluation of Recombinant Human Erythropoietin Loaded Tween 80-Albumin Nanoparticle for Traumatic Brain Injury Treatment
title_short	Preparation and Evaluation of Recombinant Human Erythropoietin Loaded Tween 80-Albumin Nanoparticle for Traumatic Brain Injury Treatment
title_full	Preparation and Evaluation of Recombinant Human Erythropoietin Loaded Tween 80-Albumin Nanoparticle for Traumatic Brain Injury Treatment
title_fullStr	Preparation and Evaluation of Recombinant Human Erythropoietin Loaded Tween 80-Albumin Nanoparticle for Traumatic Brain Injury Treatment
title_full_unstemmed	Preparation and Evaluation of Recombinant Human Erythropoietin Loaded Tween 80-Albumin Nanoparticle for Traumatic Brain Injury Treatment
title_sort	preparation and evaluation of recombinant human erythropoietin loaded tween 80-albumin nanoparticle for traumatic brain injury treatment
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/9503361fa519407f96537fcfc37800ae
work_keys_str_mv	AT xuey preparationandevaluationofrecombinanthumanerythropoietinloadedtween80albuminnanoparticlefortraumaticbraininjurytreatment AT dingj preparationandevaluationofrecombinanthumanerythropoietinloadedtween80albuminnanoparticlefortraumaticbraininjurytreatment AT liuy preparationandevaluationofrecombinanthumanerythropoietinloadedtween80albuminnanoparticlefortraumaticbraininjurytreatment AT pany preparationandevaluationofrecombinanthumanerythropoietinloadedtween80albuminnanoparticlefortraumaticbraininjurytreatment AT zhaop preparationandevaluationofrecombinanthumanerythropoietinloadedtween80albuminnanoparticlefortraumaticbraininjurytreatment AT renz preparationandevaluationofrecombinanthumanerythropoietinloadedtween80albuminnanoparticlefortraumaticbraininjurytreatment AT xuj preparationandevaluationofrecombinanthumanerythropoietinloadedtween80albuminnanoparticlefortraumaticbraininjurytreatment AT yel preparationandevaluationofrecombinanthumanerythropoietinloadedtween80albuminnanoparticlefortraumaticbraininjurytreatment AT xuy preparationandevaluationofrecombinanthumanerythropoietinloadedtween80albuminnanoparticlefortraumaticbraininjurytreatment
_version_	1718397358290501632

Preparation and Evaluation of Recombinant Human Erythropoietin Loaded Tween 80-Albumin Nanoparticle for Traumatic Brain Injury Treatment

Ejemplares similares